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  4. Recombinant Mouse Protein salvador homolog 1 (Sav1)

Recombinant Mouse Protein salvador homolog 1 (Sav1)

  • 货号：
    CSB-YP851839MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP851839MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP851839MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP851839MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP851839MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sav1; Ww45; Wwp3; Protein salvador homolog 1; 45 kDa WW domain protein; mWW45
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MLSRKKTKNE VSKPAEVQGK YVKKETSPLL RNLMPSFIRH GPTIPRRTDL CLPDSSATAF SASGDGVVSR NQSFLRTAIQ RTPHEVMRRE SHRLSAPSYL VRSLADVPRE CGSSQSFLTE VNFAVENGDS GSRYFFSDNF FDGQRRRPLG DRAQEDYRYY EYNHDLFQRM PQSQGRHTSG IGRVTATSLG NLTNHGSEDL PLPPGWSVDW TMRGRKYYID HNTNTTHWSH PLEREGLPPG WERVESSEFG TYYVDHTNKR AQYRHPCAPS VPRYDQPPPI TYQPQQTERN QSLLVPANPY HTAEIPDWLQ VYARAPVKYD HILKWELFQL ADLDTYQGML KLLFMKELEQ IVKLYEAYRQ ALLTELENRK QRQQWYAQQH GKTFLS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.
  • 基因功能参考文献：
    1. deletion of the Hippo pathway component Salvador (Salv) in mouse hearts with established ischaemic heart failure after myocardial infarction induces a reparative genetic program with increased scar border vascularity, reduced fibrosis, and recovery of pumping function compared with controls PMID: 28976966
    2. In this study, they investigated epigenetic characteristics of WW45+/- mice, evaluating time-dependent changes from prior to the onset of tumorigenesis to hepatocarcinoma. PMID: 21163252
    3. show that mice with liver-specific ablation of WW45 (a homolog of Drosophila Salvador and adaptor for the Hippo kinase) manifest increased liver size and expansion of hepatic progenitor cells (oval cells) and eventually develop hepatomas. PMID: 20404163
    4. While typical WW domains function as monomeric modules that recognize proline-rich sequences, by using conserved residues that are solvent-exposed on the surface of the beta-sheet, this WW domain buries these residues in the dimer interface. PMID: 17239860
    5. Mice lacking WW45 reveal a crucial role for WW45 in cell-cycle exit and epithelial terminal differentiation. PMID: 18369314

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Ubiquitously expressed in adult tissues with the highest level found in testis.
  • 数据库链接：

    KEGG: mmu:64010

    STRING: 10090.ENSMUSP00000021467

    UniGene: Mm.390480