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  4. Recombinant Mouse Proto-oncogene tyrosine-protein kinase LCK (Lck)

Recombinant Mouse Proto-oncogene tyrosine-protein kinase LCK (Lck)

  • 货号：
    CSB-YP012806MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012806MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012806MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012806MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012806MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lck; Lsk-tProto-oncogene tyrosine-protein kinase LCK; EC 2.7.10.2; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; p56-LCK
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-509
  • 氨基酸序列
    GCVCSSNPE DDWMENIDVC ENCHYPIVPL DSKISLPIRN GSEVRDPLVT YEGSLPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP GLHDLVRHYT NASDGLCTKL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHPRL VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLNVNK LLDMAAQIAE GMAFIEEQNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI QNLERGYRMV RPDNCPEELY HLMMLCWKER PEDRPTFDYL RSVLDDFFTA TEGQYQPQP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with UNC119; this interaction plays a crucial role in activation of LCK.
  • 基因功能参考文献：
    1. Data show that protein tyrosine kinase p56(lck) (Lck) expression is higher in Th1 cells as compared to Th2 cells. PMID: 28539628
    2. LCK nitration inhibits T cell activation, leading to reduced interleukin 2 (IL2) production and proliferation. PMID: 30232256
    3. Mutation of the basic clusters in the CD28 cytoplasmic domain reduced the recruitment to the CD28-Lck complex of protein kinase Ctheta; (PKCtheta;), which serves as a key effector kinase in the CD28 signaling pathway. PMID: 27460989
    4. A phosphosite within the SH2 Domain of Lck regulates its activation by CD45. A negative feedback loop that responds to signaling events tunes active Lck amounts and TCR sensitivity. PMID: 28735895
    5. We show a striking 65% reduction in cellularity, preferential development of gammadelta versus alphabeta T cells, and increased expression of IL-7R in the thymus of mice expressing Cre under the proximal lck promoter (lck-cre(+) mice) PMID: 27503210
    6. this work demonstrates a role for constitutive Lck activity in controlling antigen sensitivity PMID: 27271569
    7. this study defines distinct and complementary requirements for proximal and distal lck promoters during T-cell development PMID: 27469439
    8. Aurora A inhibition causes delocalized clustering of Lck at the immunological synapses and decreases its phosphorylation levels thus indicating Aurora A is required for maintaining Lck active during T-cell activation. PMID: 27091106
    9. essential for thymic development in presence or absence of Cbl, ensuring MHC restriction of T cells PMID: 25477210
    10. Lck-Dlx5 mice develop T-ALLs that consistently acquire overexpression of Myc and activation of Akt. PMID: 25793663
    11. Lck-CD8 interaction is required for initial CD8 recruitment. PMID: 25427562
    12. CD4 is expressed in distinct nanoclusters and does not colocalize with T-cell receptor and active protein tyrosine kinase p56lck PMID: 25829544
    13. Study concludes that the intracellular state of Lck Study conclude that the intracellular state of Lck determines the specificity of thymic selection and that Lck association with coreceptor proteins during thymic selection is the mechanism by which MHC restriction is imposed on a randomly generated alphabetaTCR repertoire. PMID: 24034254
    14. LCK could be considered a target attenuated by the anti-aging effects of CR. Integrative analysis of cDNA microarray and interactome data are powerful tools for identifying target molecules that are involved in the aging process and modulated by CR. PMID: 22828953
    15. Mechanistically, poly-G oligonucleotides directly induce the phosphorylation of Lck (an essential element of the T cell-signaling pathway), thereby enhancing production of IL-2 and CD8 T cell proliferation. PMID: 23296706
    16. Specific pharmacological inhibition and genetic silencing indicated the involvement of Lck in the regulation of neuritic outgrowth. PMID: 23007847
    17. Data show that activation of both tuned and untuned CD4 single-positive (SP) thymocytes is Lck-dependent. PMID: 22529380
    18. results show that Lck plays an essential role in PC in both cellular and animal models of stroke. PMID: 22623673
    19. These data illustrate a lipid microdomain-based mechanism concentrating the preactivated pool of cellular Lck and supporting its high stoichiometry of colocalization with CD45 in CD4( ) T cells. PMID: 22281390
    20. This result raises the possibility that oncogenic Lck may activate Lmo2 promoter through direct interaction. PMID: 22222369
    21. Feedback circuits monitor and adjust basal Lck-dependent events in T cell receptor signaling. PMID: 21917715
    22. These findings reveal a novel mechanistic insight into how CD28 costimulation negatively regulates the generation of induced regulatory T cells. PMID: 21245484
    23. Results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3zeta in the immunological synapse. PMID: 21103055
    24. Lck signaling is required for centrosome docking at the immunological synapse. PMID: 21339332
    25. Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. PMID: 20876849
    26. Lower levels of Lck, a key kinase involved in T cell receptor triggering, result in CD4 T cells that with wild type CD8 T cells are better able to control tumor growth. PMID: 21048115
    27. SOCS1 and SOCS3 expression attenuates Lck-mediated cellular transformation PMID: 20372794
    28. p56lck is functionally and physically associated with CD4 and CD8 in normal T lymphocytes, where an independent signal is transduced by their interaction with major histocompatibility complex (MHC) determinants. PMID: 20724731
    29. impairment of Lck-mediated CD4 coreceptor signaling by Nef is an important in vivo mechanism of HIV-1 pathogenesis PMID: 20826747
    30. CD44 directly associates with Lck in a zinc-inducible manner and this is important for the transmission of CD44-mediated signaling events leading to T cell spreading. PMID: 20417561
    31. results indicate that the cellular levels of Lck and Fyn play a role in regulating the strength of the gammadeltaTCR signaling response at different stages in the life of the gammadelta T cell PMID: 20126650
    32. These results establish that SOCS-6 acts as a negative regulator of T cell activation by promoting ubiquitin-dependent proteolysis of p56(lck). PMID: 20007709
    33. characterization of the in vivo sites of serine phosphorylation PMID: 11847223
    34. Lck, a member of the Src family of tyrosine kinases, forms a functional signaling module with PKCepsilon PMID: 11854322
    35. Long term survival of naive T cells requires a combination of signals transduced by Src family kinase member Lck (and Fyn) and signals through the IL-7 receptor. PMID: 12218114
    36. we studied intracellular signaling in SMACs by three-dimensional immunofluorescence microscopic localization of CD3, CD45, talin, phosphotyrosine, Lck and phosphorylated ZAP-70 in T cell-APC conjugates PMID: 12244310
    37. CD8(+) T cell can engage two or three targets, a synapse occurs at the site of engagement and molecules involved in attachment (lymphocyte function-associated antigen 1), signaling (Lck) and lytic activity (perforin) are differentially positioned PMID: 12352968
    38. Lck is needed for full, sustained activation of the tyrosine phosphorylation and MAP kinase cascades triggered by the BCR in CD5+, B-1 cells. Lck is involved in the higher activation threshold for productive BCR signaling in B-1 vs B-2 cells. PMID: 12645942
    39. characterization of the interdependence of Lck and Fyn function and establish the spatial and temporal distinctions of their roles in the cellular activation process PMID: 12732664
    40. the SD10 region in Lck, which is a novel site involved in substrate recognition, binds to CD45-D2 PMID: 14625311
    41. Phosphorylation of Lck induced by partial T cell receptor agonist does not lead to activation of zeta-associated protein of 70 kDa, Ca+ mobilization or cytoskeletal reorganization, yet it is deemed sufficient to transduce a competence-to-die signal. PMID: 14734719
    42. This study provides insight into the role of Lck as a mobile signaling element, whose subcellular localization indirectly predicates anti-T cell receptor (TCR)-mediated activation through its regulation of the function of colocalized Fyn. PMID: 15034040
    43. Lck-Tert mouse lines showed higher incidences of spontaneous T-cell lymphoma than the corresponding age-matched wild-type controls, indicating that constitutive expression of Tert promotes lymphoma. PMID: 15121848
    44. When signaling of naive T cells is restricted to the T cell antigen receptor, Fyn plays an essential role by positive regulation of Lck activity. PMID: 15465914
    45. Tec vesicles have a membrane proximal location and overlap with the tyrosine kinase Lck, the Tec substrate PLC-gamma1, and the early endosomal antigen 1 marker EEA1 PMID: 15817477
    46. B-1 cells expressed less Lck at both the protein and RNA levels than did B-2 cells. The same B-1 cells manifested defective BCR-mediated induction of IKKbeta phosphorylation, IkappaBalpha degradation, and intracellular Ca(2+) mobilization. PMID: 15972627
    47. the Lck binding region of SLP-76 is essential for T cell antigen receptor signaling and normal T cell development and function PMID: 16354835
    48. the cross-talk between Syk and Lck regulates hypoxia/reoxygenation-induced breast cancer progression PMID: 16474166
    49. Results indicate that Lck SH3 domain function may provide a means to independently couple receptor signaling to regulation of the ERK pathway during thymocyte development. PMID: 16923964
    50. Signals through Lck are essential for the development of T-cell-effector potential, particularly for effective cytokine transcription. PMID: 16966372

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Present at a low level in most T-cells, and at an elevated level in LSTRA and Thy19 (T-cell lymphoma) cells.
  • 数据库链接：

    KEGG: mmu:16818

    STRING: 10090.ENSMUSP00000125777

    UniGene: Mm.293753