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  4. Recombinant Mouse RalA-binding protein 1 (Ralbp1)

Recombinant Mouse RalA-binding protein 1 (Ralbp1)

  • 货号：
    CSB-YP733815MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP733815MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP733815MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP733815MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP733815MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ralbp1; Rip1; RalA-binding protein 1; RalBP1; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-648
  • 氨基酸序列
    TECFLPPSS SPSEHRRAEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP HDVLHEPPDT VSDDDKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP SKVKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ MDAPSVKPIF GVPLVDAVER TMMYDGVRLP AVFRECVDYM EKHGMKCEGV YRVSGIKSKV DELKAAYDRE ESPNLEEYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGKTTEMEKV QEFQRLLREL PECNHLLLSW LIVHLDHVIA KELETKMNIQ NISIVLSPTV QISNRVLYVL FTHVQELFGT VVLKQVTRPL RWSNMATMPT LPETQAGIKE EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME QFLRRQIASE KEEIDRLRAE IAEIQSRQQH GRSETEEYSS DSESESEDEE ELQLILEDLQ RQNEELEIKN NHLNQAVHEE REAIIELRVQ LRLLQMQRAK SEQQPQEEEE PERRGGIGPP PCDGVLEVRV AKEQAKASPS KDRKETPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L.; Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
  • 基因功能参考文献：
    1. Rlip deficiency suppresses spontaneous malignancy in p53(-/-) mice. PMID: 29572430
    2. The results show that ARNO interaction with the RLIP76 N-terminus regulates cell spreading and motility via PI3K and Arf6, independent of RLIP76 control of Rac. PMID: 25450693
    3. RLIP76 acts as a scaffold at recycling endosomes by binding activated R-Ras, recruiting ARNO to activate Arf6, thereby contributing to cell spreading and migration. PMID: 26498519
    4. The expression of Hsf4b known to be involved in lens development is down regulated in the lens of these Tg mice. PMID: 24188744
    5. a fundamental role of RLIP76 in regulating the function of obesity-promoting pro-inflammatory cytokines PMID: 23821548
    6. RalBP1 is a gene regulating the seizure threshold and synaptic inhibition in mice PMID: 23485460
    7. results establish that RLIP76 is required for efficient endothelial cell function and angiogenesis in solid tumors PMID: 22902412
    8. RLIP76 represents a mechanistically significant target for developing effective interventions in aggressive and refractory pancreatic cancers PMID: 22509328
    9. Studies confirmed a model in which RLIP76 plays a central role in the pathogenesis of metabolic syndrome and RLIP76 loss causes profound and global alterations of MSy signaling functions. PMID: 21931813
    10. Findings show a fundamental role of RLIP76 in chemical carcinogenesis. PMID: 21220488
    11. Because RLIP76 is induced by oxidative stress, it could play a role in insulin resistance seen in pathological conditions characterized by increased oxidative stress PMID: 20007934
    12. Data show that RLIP76 knockout mice are extremely sensitive to irradiation. PMID: 19626587
    13. role in regulating endocytosis of activin type II receptors by ARIP2 PMID: 11882656
    14. RIP1 may mediate the p38 MAP kinase response to TNF-alpha, by recruiting the MAP3K MEKK3. PMID: 14585994
    15. Poly(ADP-ribose)polymerase-1 signaling to mitochondria in necrotic cell death requires Rip1-mediated JNK1. PMID: 16446354
    16. Thus, we identify a novel R-Ras effector, RLIP76, which links R-Ras to adhesion-induced Rac activation through a GTPase cascade that mediates cell spreading and migration. PMID: 16966426
    17. Loss of RLIP76 confers sensitivity to xenobiotics and radiation due to the loss of a common transport mechanism for glutathione-electrophile conjugates and xenobiotics. PMID: 18789326

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Mitochondrion.
  • 组织特异性：
    Ubiquitous. The highest level of expression was observed in ovaries and skeletal muscle, whereas the lowest was found in spleen, liver and peripheral blood leukocytes.
  • 数据库链接：

    KEGG: mmu:19765

    STRING: 10090.ENSMUSP00000024905

    UniGene: Mm.17009