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  4. Recombinant Mouse Ras-related protein Rab-10 (Rab10)

Recombinant Mouse Ras-related protein Rab-10 (Rab10)

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  • 中文名称：
    小鼠Rab10重组蛋白
  • 货号：
    CSB-EP019152MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rab10; Ras-related protein Rab-10
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    38.5kDa
  • 表达区域：
    1-200aa
  • 氨基酸序列
    MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane. In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation. May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, it binds RILPL1 and inhibits ciliogenesis.
  • 基因功能参考文献：
    1. These results implicate the stress-responsive machinery composed of Rab7L1, LRRK2, phosphorylated Rab8/10, and their downstream effectors in the maintenance of lysosomal homeostasis. PMID: 30209220
    2. Rab10 plays a role in oligodendrocyte precursor cell maturation. PMID: 28132130
    3. SEC16A and RAB10 promote insulin-stimulated mobilization of GLUT4 from a perinuclear recycling endosome/trans Golgi network compartment. PMID: 27354378
    4. Glut4 traffics predominantly through the specialized Rab10-dependent pathway both before and after insulin stimulation. PMID: 26527681
    5. role of Rab10 in mouse embryo PMID: 25860786
    6. These results suggest that the association of Rab10-GTP with Exoc6/6b is a molecular link between insulin signaling and the exocytic machinery in GLUT4 translocation. PMID: 26299925
    7. RAB10 and its GTPase-activating protein AS160 comprise the principal signaling module downstream of insulin receptor activation that regulates the accumulation of GLUT4 transport vesicles at the plasma membrane. PMID: 23804653
    8. Rab10 coordinates with myosin-Va to mediate the final steps of insulin-stimulated vesicle translocation to the plasma membrane. PMID: 22908308
    9. show that continuous replenishment of TLR4 from Golgi to plasma membrane is regulated by the small GTPase Rab10, which is essential for optimal macrophage activation following ipopolysaccharide stimulation. PMID: 20643919
    10. Rab10 is a target of AS160 and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation. PMID: 17403373
    11. the subcellular distribution of Rab10, the fraction of Rab10 in the active GTP form in vivo; Rab10 participates in GLUT4 translocation in 3T3-L1 adipocytes PMID: 18076383

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasm, cytoskeleton, cilium basal body. Endoplasmic reticulum membrane. Cytoplasm, perinuclear region.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Expressed in the brain, specifically neurons (at protein level).
  • 数据库链接：

    KEGG: mmu:19325

    STRING: 10090.ENSMUSP00000021001

    UniGene: Mm.378993