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  4. Recombinant Mouse Ras-related protein Rab-27A (Rab27a)

Recombinant Mouse Ras-related protein Rab-27A (Rab27a)

  • 中文名称：
    小鼠Rab27a重组蛋白
  • 货号：
    CSB-YP861694MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rab27a重组蛋白
  • 货号：
    CSB-EP861694MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rab27a重组蛋白
  • 货号：
    CSB-EP861694MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rab27a重组蛋白
  • 货号：
    CSB-BP861694MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rab27a重组蛋白
  • 货号：
    CSB-MP861694MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rab27a
  • Uniprot No.：
  • 别名：
    Rab27a; Ras-related protein Rab-27A; EC 3.6.5.2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-221
  • 氨基酸序列
    MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRANGPDG AVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHTSADQL SEEKEKGLCG C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • 基因功能参考文献：
    1. IRF-1 regulates Rab27a transcription and extracellular vesicles secretion, leading to oxidized phospholipids activation of neutrophils and subsequent hepatic ischemia reperfusion injury PMID: 29059701
    2. miR-124 has an important role in osteoclastogenesis via targeting Rab27a. PMID: 29045940
    3. this study shows that Rab27a and Rab27b double-knockout mice that are deficient in exosome secretion have a chronic, low-grade inflammatory phenotype characterized by elevated inflammatory cytokines and myeloproliferation PMID: 28978688
    4. Rab27a is a possible target for intervention in inflammatory processes in which GM-CSF-dependent neutrophil priming is involved. PMID: 27733578
    5. These results suggest that the dual effector, Noc2, regulates the transition from Rab2a-mediated granule biogenesis to Rab27a-mediated granule exocytosis. PMID: 27927751
    6. Loss of Rab3D from secretory vesicles, leading to disproportionate Rab27-to-Rab3D activity, may contribute to the enhanced release of cathepsin S in tears of patients with Sjogren's syndrome. PMID: 27076615
    7. identified small molecule inhibitors of Rab27a-JFC1 binding that were also active in cell-based neutrophil-specific exocytosis assays, demonstrating the druggability of Rab GTPases and their effectors. PMID: 27702998
    8. The Rab27A regulates CG exocytosis following MII-stage oocyte activation. PMID: 26791531
    9. MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A. PMID: 28043921
    10. Given that GTP- and GDP-bound Rab27a regulate exocytosis. PMID: 26683831
    11. This paper showed an inhibitory effect of Rab27a directly at the pore forming CaV1.3 subunit of L-type channels, leading to decreased current density and reduced VEGF-A secretion by the retinal pigment epithelium. PMID: 26235199
    12. results show that Rab27A is present in mouse pancreatic acinar cells and mainly regulates secretion through the minor-regulated pathway PMID: 25951179
    13. Rab27A deficiency shows the increased surface level of c-fms, and the delayed downregulation of RANK in ashen cells, resulting in enhanced signaling of M-CSF and RANKL. PMID: 25882854
    14. Rab27b is expressed in bone marrow derived neutrophils and Rab27b knockout (Rab27b KO) along with Rab27a/b double knockout (Rab27DKO) neutrophils exhibit impaired transwell migration in vitro in response to chemokines MIP-2 and LTB4. PMID: 25359237
    15. Our findings indicate that Slp2-a controls renal cell size through regulation of Rap-ezrin signaling independently of Rab27. PMID: 24284068
    16. The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin. PMID: 24584932
    17. Rab27a has a key role in eosinophil degranulation. Furthermore, these findings have implications for Rab27a-dependent eosinophil degranulation in airway inflammation. PMID: 23986549
    18. Upregulation of the Rab27a-dependent lysosomal trafficking and secretory pathways contributes to the correction of some of the cellular defects induced by lysosomal overload in cystinosis, including endoplasmic reticulum stress. PMID: 23716592
    19. Data suggest that Rab27a and Rab27b regulate distinct steps in the bone marrow-derived mast cells (BMMC) degranulation pathway, with Rab27a/Mlph/MyoVa regulating cortical actin stability upstream of Rab27a/b/Munc13-4-dependent granule exocytosis. PMID: 23281710
    20. Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11. PMID: 23160464
    21. The Rab27a-dependent protease secretion regulates neutrophil migration through proteolysis-dependent de-adhesion of uropods, a mechanism that could be conserved in cell migration and invasion. PMID: 22375060
    22. Slp2-a is required for targeting of the signaling molecule podocalyxin to the apical membrane in a Rab27A-dependent manner. PMID: 22767581
    23. Data indicate that inhibition of Rab27a, but not Rab27b, affects exosome secretion. PMID: 22865453
    24. Using Rab27a mutants that show impaired binding to representatives of all four Rab27a effector subgroups, we present evidence that effector binding is not essential for targeting of Rab27a to melanosomes. PMID: 21554507
    25. our data indicate that Rab27a plays an important role in neutrophil recruitment to liver and LPS-induced death during endotoxemia, thus highlighting a previously unrecognized role for Rab27a in LPS-mediated systemic in fl ammation. PMID: 21746860
    26. Mobilization of preformed CD40 ligand (CD40L) does not depend on Rab27a, which is required for mobilization of lytic granules. PMID: 21677130
    27. Stimulation-dependent lysosomal RANKL release is mediated by Rab27a/b and their effector molecules, and this mechanism may be important for osteoclast activation in vivo. PMID: 20939018
    28. Rab27a hasa role in the CMV life cycle and CMV and LRO biogenesis share common molecular mechanisms PMID: 21170347
    29. Morphological changes observed at the ultrastructural level in Rab27a-deficient lung samples result from secretory defects in alveolar epithelial type II and Clara cells that, over time, lead to atrophy of the epithelium. PMID: 21160031
    30. Munc13-4 restricts motility of Rab27a-expressing vesicles to facilitate lipopolysaccharide-induced priming of exocytosis. PMID: 21148308
    31. Loss of Rab27a increased the fraction of mobile lytic granules and the extent of their movement in the cytosol. PMID: 20877725
    32. Both Rab27a and Rab27b deficiencies impaired azurophilic granule exocytosis. Rab27a(ash/ash) neutrophils showed upregulation of Rab27b expression which did not compensate for the secretory defects observed in Rab27a-deficient cells. PMID: 20028487
    33. Coronin 3 is a genuine GDP-Rab27a effector, and that controls endocytosis of the secretory membrane via modulating actin assembly in pancreatic beta-cells. PMID: 20138020
    34. The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes PMID: 11886590
    35. myosin Va binds to melanophilin and Rab27a PMID: 11887186
    36. The regulation of platelet-dense granules by Rab27a in the ashen mouse, a model of Hermansky-Pudlak and Griscelli syndromes, is granule-specific and dependent on genetic background. PMID: 12070017
    37. Rab27b is functionally redundant with Rab27a and the pathogenesis of Griscelli syndrome is determined by the relative expression of Rab27a and Rab27b in specialized cell types. PMID: 12122117
    38. recognition of the GTP-bound form of Rab27A requires the SHD1 of Slac2-a/melanophilin PMID: 12189142
    39. Rab27a acts in concert with Rab3 proteins in most regulated secretory events PMID: 14617806
    40. The acroplaxome provides a focal point for myosin-Va/ Rab27a/b-driven proacrosomal vesicles to accumulate, coalesce, and form an acrosome in wild-type spermatids and a pseudoacrosome in Hrb mutant spermatids. PMID: 14724135
    41. Rab27a regulates melanosome distribution in the retinal pigment epithelial cells. PMID: 14978221
    42. differential and sequential roles of Rab27A-binding proteins in melanosome transport in melanocytes. PMID: 15543135
    43. Rab27a/effector system mediates glucose-specific signals for the exocytosis of insulin granules in pancreatic beta cells. PMID: 15690086
    44. Slac2-c acts as a functional myosin VIIa receptor and the Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates the transport of retinal melanosomes in pigment epithelium cells PMID: 15927964
    45. EPI64 is a GTPase-activating protein specific for Rab27A PMID: 16923811
    46. Rab27a and JFC1/Slp1 permit myeloperoxidase release into the surrounding milieu and constitute key components of the secretory machinery of azurophilic granules in granulocytes PMID: 17090228
    47. Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes. PMID: 17351642
    48. Data suggest that Mlph is targeted to and/or stabilised on melanosomes by Rab27a, and then recruits MyoVa, which provides additional stability to the complex and allows melanosomes to transfer from MT to actin-based transport. PMID: 17698919
    49. These results show that RAB27A is a new direct transcriptional target of MITF and link MITF to melanosome transport, another key parameter of melanocyte differentiation and skin pigmentation. PMID: 18281284
    50. Rab3GEP has a role as the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes PMID: 18559336

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  • 亚细胞定位：
    Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Detected in melanocytes. Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the thymus and lung.
  • 数据库链接：

    KEGG: mmu:11891

    STRING: 10090.ENSMUSP00000034722

    UniGene: Mm.34867