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  4. Recombinant Mouse Ras-related protein Rab-27B (Rab27b)

Recombinant Mouse Ras-related protein Rab-27B (Rab27b)

  • 中文名称：
    小鼠Rab27b重组蛋白
  • 货号：
    CSB-YP860000MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rab27b重组蛋白
  • 货号：
    CSB-EP860000MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rab27b重组蛋白
  • 货号：
    CSB-EP860000MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rab27b重组蛋白
  • 货号：
    CSB-BP860000MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rab27b重组蛋白
  • 货号：
    CSB-MP860000MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rab27b
  • Uniprot No.：
  • 别名：
    Rab27b; Ras-related protein Rab-27B; EC 3.6.5.2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-218
  • 氨基酸序列
    TDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYDTQGADG ASGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL IMKRMEKCVE KTQVPDTVNG GNSGKLDGEK PAEKKCAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1. May be involved in targeting uroplakins to urothelial apical membranes.
  • 基因功能参考文献：
    1. While RAB11a recruits its effector RAB11FIP3 and cytoskeletal motor Dynein, RAB27b mobilizes the effector MyRIP and motor Myosin VIIa to mediate bacterial expulsion. PMID: 28910634
    2. this study shows that Rab27a and Rab27b double-knockout mice that are deficient in exosome secretion have a chronic, low-grade inflammatory phenotype characterized by elevated inflammatory cytokines and myeloproliferation PMID: 28978688
    3. PI3K-dependent formation of a kinesin-1/Slp3/Rab27b complex is critical for the microtubule-dependent movement of secretory granules required for mast cell degranulation. PMID: 27810912
    4. Loss of Rab3D from secretory vesicles, leading to disproportionate Rab27-to-Rab3D activity, may contribute to the enhanced release of cathepsin S in tears of patients with Sjogren's syndrome. PMID: 27076615
    5. Rab27b is expressed in bone marrow derived neutrophils and Rab27b knockout (Rab27b KO) along with Rab27a/b double knockout (Rab27DKO) neutrophils exhibit impaired transwell migration in vitro in response to chemokines MIP-2 and LTB4. PMID: 25359237
    6. Expression of Rab27b DN constructs resulted in dispersed collagen vesicles which may represent failed secretory vesicles sequestered in the cytosol. PMID: 23050002
    7. Data indicate that inhibition of Rab27a, but not Rab27b, affects exosome secretion. PMID: 22865453
    8. Rab27b participates in aspects of lacrimal gland acinar cell secretory vesicle formation and release. PMID: 21525430
    9. Stimulation-dependent lysosomal RANKL release is mediated by Rab27a/b and their effector molecules, and this mechanism may be important for osteoclast activation in vivo. PMID: 20939018
    10. Morphological changes observed at the ultrastructural level in Rab27b-deficient lung samples result from secretory defects in alveolar epithelial type II and Clara cells that, over time, lead to atrophy of the epithelium. PMID: 21160031
    11. Both Rab27a and Rab27b deficiencies impaired azurophilic granule exocytosis. Rab27a(ash/ash) neutrophils showed upregulation of Rab27b expression which did not compensate for the secretory defects observed in Rab27a-deficient cells. PMID: 20028487
    12. Chromosomal mapping and gene structure of the RAB27B gene. PMID: 11178108
    13. Rab27b is involved in pituitary hormone secretion PMID: 11956164
    14. Rab27b may regulate the outward movement of melanosomes and the formation or maintenance of dendritic extensions in melanocytes. PMID: 12060386
    15. Rab27b is functionally redundant with Rab27a and the pathogenesis of Griscelli syndrome is determined by the relative expression of Rab27a and Rab27b in specialized cell types. PMID: 12122117
    16. Rab27b in particular may coordinate proplatelet formation with granule transport, possibly by recruiting specific effector pathways PMID: 12907454
    17. plays a role in regulating the delivery of fusiform vesicles to the apical plasma membrane of umbrella cells of bladder epithelium PMID: 14625374
    18. The acroplaxome provides a focal point for myosin-Va/ Rab27a/b-driven proacrosomal vesicles to accumulate, coalesce, and form an acrosome in wild-type spermatids and a pseudoacrosome in Hrb mutant spermatids. PMID: 14724135
    19. Rab27b regulates number and secretion of platelet dense granules. PMID: 17384153
    20. Rab27b-GDP exists in an open conformation with protruding switch and interswitch regions PMID: 17582168
    21. Rab27 proteins, particularly Rab27b, play a crucial role in mast cell degranulation and their action regulates the transition from microtubule to actin-based motility PMID: 17587407
    22. Rab27 subfamily is involved in the delivery of granules near the exocytic site PMID: 17761531
    23. Structural basis for the exclusive association of Slac2-a with the Rab27 subfamily. PMID: 18940604

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  • 亚细胞定位：
    Membrane; Lipid-anchor. Late endosome.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the brain and spleen.
  • 数据库链接：

    KEGG: mmu:80718

    STRING: 10090.ENSMUSP00000068349

    UniGene: Mm.246753