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  4. Recombinant Mouse Ras-related protein Rab-3A (Rab3a)

Recombinant Mouse Ras-related protein Rab-3A (Rab3a)

  • 货号：
    CSB-YP019194MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019194MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019194MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019194MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019194MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rab3a; Ras-related protein Rab-3A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-220
  • 氨基酸序列
    MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG AKQGPQLTDQ QAPPHQDCAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 isoform RIMS2 and RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.
  • 基因功能参考文献：
    1. Rab3A might function as a marker of cortical granules and participates in cortical granule exocytosis in mouse eggs. PMID: 27423421
    2. Rab3a accelerates cell proliferation by increasing cyclin D1 expression, enhances anti-cancer drug resistance, and increases tumorigenicity and self-renewal of glioma cells. PMID: 24965146
    3. Rab3a deletion restores excitatory/inhibitory imbalance in synapsin II(-) hippocampal slices. PMID: 24227741
    4. betaARs couple to a cAMP/Epac/PLC/Munc13-1/Rab3a/RIM1a-dependent pathway to enhance glutamate release at cerebrocortical nerve terminals. PMID: 24036110
    5. key role of Rab3a proteins control the balance between secretory vesicle biogenesis and degradation, and suggest that the ablation of this protein probably changes the nature of the reservoir of secretory vesicles available for regulated exocytosis. PMID: 23399968
    6. alpha-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity PMID: 23344955
    7. Data show diminished actin plasticity in the cell processes of Rab3A knockout (KO) podocytes. PMID: 22472623
    8. these findings suggest a crucial role of Rab3A in delivering vesicles to Ca2+ -dependent release sites at ribbon synapses. PMID: 22593061
    9. We conclude that Rab3A is involved in a novel presynaptic mechanism to homeostatically regulate the amount of transmitter in a quantum. PMID: 21389214
    10. roles in maintaining the total store of synaptic vesicles and cooperate in promoting the latest steps of neuronal secretion. PMID: 20338242
    11. We suggest that Rab3A may regulate SNAP-29-mediated membrane fusion during myelination PMID: 19170188
    12. Data show that Rab3A is highly and specifically expressed in brain regions. PMID: 19570198
    13. The monomeric GTP binding protein, rab3a, is associated with the acrosome in mouse sperm. PMID: 10398417
    14. role in regulatory mechanism of neurotransmitter release PMID: 11871104
    15. Phenotypic assessment of earlybird mice and a null allele of Rab3a revealed anomalies in circadian period and sleep homeostasis, providing evidence that Rab3a-mediated synaptic transmission is involved in these behaviors PMID: 12244319
    16. Rab3A protein has a role in exocytosis PMID: 12438138
    17. Rab3A reduces the potency of adenosine as an endogenous mediator of neuromuscular depression PMID: 12456815
    18. Rab3A plays an important in vivo role facilitating the efficiency of insulin exocytosis, most likely at the level of replenishing the ready releasable pool of beta-granules PMID: 12510060
    19. a possible role in glomerulopathies PMID: 12937130
    20. Mice deficient for the synaptic vesicle protein Rab3a show impaired spatial reversal learning and increased explorative activity. PMID: 15078563
    21. Rab3A and its effectors are general modules for four distinct types of PKA-dependent LTP in the brain PMID: 15967982
    22. Rab3-induced modifications to primed vesicles causes a transient increase in the transduction efficacy of synaptic action potential trains and optimizes the encoding of synaptic information at an intermediate spike frequency range. PMID: 16436611
    23. Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2 PMID: 16704978
    24. Data implicate Rab3A in learning and memory as well as in the regulation of emotion. Differences in genetic background have a stronger effect on gene expression than the mutations in the Rab3a gene. PMID: 16734774
    25. In p130-deficient mice the GTP-bound form of Rab3A accumulated in the brain. PMID: 16782817
    26. Rab3a positively regulates docking and basal quantal release at the mouse neuromuscular junction. This result is consistent with the proposed role of rab3a in trafficking and targeting vesicles to the active zones. PMID: 17640821
    27. Rab3GEP has a role as the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes PMID: 18559336
    28. We found that small amplitude pre-spike feet with abnormally long durations were increased in Rab3A(-/-) cells. PMID: 18591190
    29. Results support the hypothesis that Rab3a maintains the pool of fusion competent vesicles tightly coupled to Ca2+ channels. PMID: 19348946

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Cytoplasmic vesicle, secretory vesicle. Cell projection, axon. Cell membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Found in active zone in brain nerve terminals.
  • 数据库链接：

    KEGG: mmu:19339

    STRING: 10090.ENSMUSP00000034301

    UniGene: Mm.5083