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  4. Recombinant Mouse Ras-related protein Rab-7a (Rab7a)

Recombinant Mouse Ras-related protein Rab-7a (Rab7a)

  • 中文名称：
    小鼠Rab7a重组蛋白
  • 货号：
    CSB-YP019219MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rab7a重组蛋白
  • 货号：
    CSB-EP019219MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rab7a重组蛋白
  • 货号：
    CSB-EP019219MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rab7a重组蛋白
  • 货号：
    CSB-BP019219MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rab7a重组蛋白
  • 货号：
    CSB-MP019219MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rab7a; Rab7; Ras-related protein Rab-7a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-207
  • 氨基酸序列
    TSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA SAESCSC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surface expression of ACE2. May play a role in PRPH neuronal intermediate filament assembly.
  • 基因功能参考文献：
    1. Rab7 deficiency exacerbates the severity of acute pancreatitis by impairing the autophagic and endocytic pathways toward lysosomes. PMID: 28588238
    2. High Rab7 expression is associated with tumor growth and metastasis. PMID: 28924047
    3. WDR91 serves as a Rab7 effector that is essential for neuronal development by facilitating endosome conversion in the endosome-lysosome pathway. PMID: 28860274
    4. Vps34 has a previously unknown role in regulating Rab7 activity and late endosomal trafficking. PMID: 27793976
    5. Rab7 accumulated in GCase deficient cells, supporting the notion that lysosomal recycling is impaired. Since recombinant GCase can reverse ALR impairment, we anticipate that strategies to restore GCase activity in the brains of both sporadic patients with PD and those with GBA1 mutations will improve autophagy lysosomal pathway, preventing the accumulation of a-synuclein and spread of pathology. PMID: 27378698
    6. The inhibition of autophagosome-lysosome fusion induced by SapM is dependent on the interaction between SapM and Rab7. PMID: 27609571
    7. Prion infection impairs lysosomal degradation capacity by interfering with rab7 membrane attachment in neuronal cells. PMID: 26865414
    8. photoreceptor outer segments vesicles engulfed through Lyar-dependent pathway were targeted to phagosomes and colocalized with phagosome marker Rab7 PMID: 25735755
    9. B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses. PMID: 25740947
    10. CREG1 deficiency influenced the maturation of lysosomes and reduced the espression of Rab7, which might be involved in CREG1-induced cardiomyocyte autophagy. PMID: 25774384
    11. Tropheryma whipplei blocks the switch from Rab5 to Rab7. PMID: 24586722
    12. ADRB2 stimulation has caused a marked increase in the autophagy-targeted lipid droplets (LD) for lysosomal degradation, which is dependent on the LD-associated RAB7. PMID: 23708524
    13. Loss of rab7 is better tolerated in naive T cells than the loss of atg5. PMID: 23615463
    14. Rab7 is required for LPS-stimulated lysosome tubulation in macrophages. PMID: 22909026
    15. Signaling protein ras-related protein rab-7A and septin 8 levels were significantly higher in hippocampus of poor contextual fear conditioning extinguishers. PMID: 21770032
    16. Rab7a is an interacting partner of cellular prion protein in neuronal cells. PMID: 21604690
    17. Results suggest that Rab7 may play a role in secretory lysosome movement toward the centrosome by interacting with RILP to recruit the minus-end motor, dynein. PMID: 21438969
    18. Valproic acid improves defective neurite formation in neuroblastoma cells regardless of which Charcot-Marie-Tooth disease-associated Rab7 mutant protein is expressed, an effect mediated via the c-Jun N-terminal kinase (JNK) signaling pathway. PMID: 20645406
    19. A novel role is demonstrated for SP-A in modulating endolysosomal trafficking via Rab7b (but not Rab5 nor Rab11) in primary alveolar macrophages, and the biochemical pathways are defined. PMID: 21248257
    20. although only TBC1D15/Rab7-GAP altered Rab7-GTP levels, both Rab7-GAP and mVps39 regulate lysosomal morphology and play a role in maintaining growth factor dependence PMID: 20363736
    21. CD63, but not RILP, is recruited to the phagosomes in macrophages expressing the dominant negative form of Rab7. PMID: 20236428
    22. Phosphatidylinositol 3-kinase modulates Rab5 and Rab7 recruitment to phagosomes PMID: 12640132
    23. Results show a role for Rab7 in the final maturation of late autophagic vacuoles. PMID: 15340014
    24. Therefore, Rab7 plays an essential role in axonal retrograde transport by controlling a vesicular compartment implicated in neurotrophin traffic. PMID: 17046692
    25. cholesterol accumulation can have a detrimental effect on phagosome maturation by impairing the activation of Rab7 PMID: 18955491
    26. the late endosome/lysosome-localized Rab7b can down-regulate TLR9-triggered proinflammatory cytokine and type I IFN production by impairing TLR9 signaling via promotion of TLR9 degradation PMID: 19587007

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  • 亚细胞定位：
    Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein; Cytoplasmic side. Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Melanosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet. Endosome membrane. Cytoplasmic vesicle.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Widely expressed. High expression in liver, heart and kidney. Found in sensory and motor neurons.
  • 数据库链接：

    KEGG: mmu:19349

    STRING: 10090.ENSMUSP00000092658

    UniGene: Mm.333233