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  4. Recombinant Mouse Ras-related protein Ral-A (Rala)

Recombinant Mouse Ras-related protein Ral-A (Rala)

  • 货号：
    CSB-YP019296MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019296MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019296MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019296MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019296MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rala; Ral; Ral-a; Ras-related protein Ral-A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-203
  • 氨基酸序列
    MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM EDSKEKNGKK KRKSLAKRIR ERC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody. During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1.
  • 基因功能参考文献：
    1. RalA plays a crucial role in glucose transport in adipose tissue in vivo. PMID: 29915037
    2. This study identifies a novel regulatory crosstalk between Ral and Arf6 that controls Ral function in cells. PMID: 27269287
    3. striking isoform-specific consequences of distinct CAAX-signaled posttranslational modifications that contribute to the divergent subcellular localization and activity of RalA and RalB. PMID: 26216878
    4. RalA activation was remarkably impaired in rac1-deficient skeletal muscle fibres. PMID: 26205497
    5. Our results provide the first in vivo characterization of RalA function in the mammalian brain and highlight a novel molecular mechanism for cell polarization. PMID: 24284074
    6. The constitutively increased RalA activity occludes further increases in RalA activity during induction of long-term depression, causing impaired NMDAR-long-term depression. PMID: 23433113
    7. findings show either RALA or RALB is sufficient for tumor growth; either RALA or RALB is sufficient for cell proliferation; RALA and RALB act in a redundant fashion PMID: 23063435
    8. study reports the identification and characterization of a Ral GAP complex (RG1, RGC2) that mediates the activation of RalA downstream of the PI 3-kinase/Akt pathway PMID: 21148297
    9. A novel regulatory pathway involves RalA and phospholipase D in the production of phosphatidic acid during Fc gamma receptor-mediated phagocytosis and phagosome formation. PMID: 20679536
    10. RalA but not RalB mediates integrin-dependent membrane raft exocytosis through the exocyst complex. Constitutively active RalA restores membrane raft targeting to promote anchorage-independent growth signaling. PMID: 20005108
    11. RalA activation elicited by the exchange factor RalGDS in response to a rise in intracellular Ca2+ and cAMP controls hormone release from pancreatic beta-cells PMID: 19890390
    12. RalA is activated by H-Ras, which along with ARF6, leads to phospholipase D activation PMID: 12509462
    13. RalA is a critical component in biphasic insulin release from pancreatic beta cells PMID: 18426794
    14. Silencing Ral or inhibiting it with a dominant-negative Ral (Ral S28N) caused a significant reduction in proliferation, invasiveness, and in vivo tumorigenicity of malignant peripheral nerve sheath tumors. PMID: 19414599
    15. Exocytosis pathway seems to be particularly important for M-induced nanotube formation, because interfering with the RalA. PMID: 19935652

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring. Mitochondrion.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 数据库链接：

    KEGG: mmu:56044

    STRING: 10090.ENSMUSP00000009003

    UniGene: Mm.27348