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  4. Recombinant Mouse Ras-related protein Rap-1A (Rap1a)

Recombinant Mouse Ras-related protein Rap-1A (Rap1a)

  • 货号：
    CSB-YP019321MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019321MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019321MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019321MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019321MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rap1a; Krev-1; Ras-related protein Rap-1A; Ras-related protein Krev-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-181
  • 氨基酸序列
    MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
  • 基因功能参考文献：
    1. Using an RNA-mediated interference screen, we identified phospholipase Cepsilon 1 (PLCepsilon1) as a crucial regulator of stromal cell-derived factor 1 alpha (SDF-1alpha)-induced Rap1 activation. We have shown that SDF-1alpha-induced activation of Rap1 is transient in comparison with the sustained level following cross-linking of the antigen receptor. PMID: 28213494
    2. loss of Rap1 GTPases prevents the formation of axons and leading processes and thereby interferes with radial migration PMID: 26733533
    3. neuronal Rap1 critically regulates leptin sensitivity PMID: 27626668
    4. the Rap1-cofilin-1 pathway coordinates actin and microtubule organization at the immune synapse. PMID: 28167682
    5. Rap1 mediates the effects of increased extracellular tension in multiple ways that are capable of contributing to tumor progression when dysregulated. PMID: 27199371
    6. Results from comparative proteomics identified Rap1 as a novel legionella-containing-vacuole host component implicated in intracellular replication of Legionella pneumophila. PMID: 28183814
    7. These results suggest that Rap1 activation of ERKs requires PKA phosphorylation and KSR binding. PMID: 28003362
    8. the interaction between nephrin and MAGI-1 regulates Rap1 activation in podocytes to maintain long term slit diaphragm structure PMID: 27707879
    9. These findings ascribe a new function to SLAT, and identify Rap1 as a target of SLAT function in TCR-mediated inside-out signaling. PMID: 26483383
    10. Rap1 Is Essential for Mouse Development and Formation of Functional Vasculature PMID: 26714318
    11. Rap1 deficiency impaired T-cell homeostasis. PMID: 26634692
    12. Rap1 promotes endothelial mechanosensing complex formation, NO release and normal endothelial function PMID: 25807985
    13. these data show that Treg degrade ATP to adenosine via CD39, attracting DC by activating Epac1-Rap1-dependent pathways. PMID: 25780038
    14. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. PMID: 25533468
    15. PI3-kinase gamma promotes Rap1a-mediated activation of myeloid cell integrin alpha4beta1, leading to tumor inflammation and growth. PMID: 23565202
    16. Rap1 has distinct patterns of signaling in normal vascular morphogenesis and dysfunction. (Review) PMID: 23911990
    17. Rap1 inhibits cell migration by suppression of RhoA activity in response to TGF-beta1. PMID: 23559363
    18. Rap1 signaling enables plasticity by regulating L-type calcium channels at cortico-amygdala synapses. PMID: 23616533
    19. Talin1 and Rap1 are critical for resorptive function, and their selective inhibition in mature osteoclasts retards pathological bone loss. PMID: 23230271
    20. Rap1 activation restricts autoimmune T cell-mediated pathology through enhancing tolerance. PMID: 22688423
    21. demonstrate that in rap1-deficient T cells, signaling by the ERK and p38 kinases is increased following activation by different stimuli leading to increased intracellular accumulation and secretion of cytokines PMID: 22695258
    22. Id-1 maintains the GTPase activity of Rap1. PMID: 22647934
    23. both Rap1 and RhoA GTPases regulate the formation of filamentous actin through the interaction between actin and profilin, thereby collectively inducing the phagocytosis of SOZs in macrophages. PMID: 22194606
    24. farnesylthiosalicylic acid-amide inhibits contact sensitivity through Rap1 inhibition PMID: 21716322
    25. data demonstrate that Rap1a activation by p38 MAPK and CalDAG-GEFI is involved in E-selectin-dependent slow rolling and leukocyte recruitment PMID: 21480213
    26. Data show that Rap1 regulated the plasma membrane localization of NCad and NCad rescued radial polarization when Rap1 was inhibited. PMID: 21516100
    27. cAMP-responsive Rap1 guanine nucleotide exchange factor, Epac, induces smooth muscle relaxation by down-regulation of RhoA activity PMID: 21454546
    28. demonstrate that pharmacological activation of Epac-Rap signaling using acetoxymethyl-8-pCPT-2'-O-Me-cAMP preserves cell adhesions during hypoxia in vitro PMID: 21493776
    29. Rap1A increases the frequency of natural Treg in the thymus via LFA-1- independent mechanisms but affects the frequency of peripheral Treg predominantly in an LFA-1-dependent manner. PMID: 20864093
    30. maintenance of T cell Rap1 signaling in murine T cells reduces disease incidence and severity in collage-induced arthritis, which are associated with specific defects in T cell effector function. PMID: 20662068
    31. IL-8-induced NAADP production is mediated by CD38 activation through the actions of cAMP/Epac/protein kinase A/Rap1 in LAK cells, and NAADP plays a key role in Ca(2+) signaling of IL-8-induced LAK cell migration PMID: 20442403
    32. findings reveal the several critical steps of Rap1, which are RAPL-dependent and -independent, in lymphocyte trafficking PMID: 19965628
    33. The results indicate that afadin has a novel role in PDGF-induced directional cell movement, presumably in cooperation with active Rap1 and SHP-2. PMID: 19887591
    34. show that Rap1 activation is important for circulating lymphoma cells to enter tissues and form tumors in vivo. PMID: 19838206
    35. These data describe for the first time protective effects for Rap1-dependent signaling against ventilator-induced lung injury and pulmonary endothelial barrier dysfunction. PMID: 19850733
    36. CTLA-4 activates Rap1, and we propose that intracellular signals from CTLA-4 antagonize CD28, at least in part, at the level of Rap1 PMID: 15870282
    37. Rap1A plays role in the regulation of integrins. PMID: 16382154
    38. TIMP-2 mediated alteration in cell morphology requires Rap1, TIMP-2 may recruit Rap1 to sites of actin cytoskeleton remodeling necessary for cell spreading, and enhanced cell adhesion by TIMP-2 expression may hinder cell migration PMID: 16716258
    39. Despite 95% amino acid sequence identity, similar intracellular distribution, and broad tissue distribution, Rap1a and 1b are not functionally redundant but rather differentially regulate certain cellular events. PMID: 18056377
    40. The results suggested that deregulated constitutive Rap1 activation caused abnormal expansion of DP thymocytes, bypassing the pre-T-cell receptor and eventually leading to Notch1 mutations and Notch-dependent T-cell acute lymphoblastic leukemia. PMID: 18180377
    41. Rap1a-mediated suppression of synaptic transmission enables plasticity in the cortico-amygdala pathway, which is required for fear learning with a moderately aversive unconditional stimulus. PMID: 18305243
    42. our findings are consistent with the model describing the connection of c-Cbl to the cytoskeletal rearrangements via two pathways, one of which is mediated by PI3K and Rac1, and the other, by CrkL/C3G, Rap1 and Rac1 PMID: 18403249
    43. Results indicate that Rap1, activated by PDGF, is recruited to leading edges and that Rac1 is thereby activated locally at peripheral ruffles; this process is pivotal for the PDGF-induced formation of leading edge structures and cell movement. PMID: 18422604
    44. GTP-Rap1 is required for pancreatic amylase release. Rap1 activation not only mediates the cAMP-evoked response via Epac1 but is also involved in CCK- and carbachol-induced amylase release, with their action most likely mediated by CalDAG-GEF III. PMID: 18577515
    45. Results suggest that Rap1a is a key regulator of fibroblast growth factor 2-induced angiogenesis and together with Rap1b controls endothelial cell functions. PMID: 18625726
    46. Rap1a and Rap1b are essential for the conformational activation of beta1-integrins in endothelial cells PMID: 18805968
    47. Results are consistent with a cAMP/Epac2/Rap1-mediated signaling that could exert its action, among others, through RA-RhoGAP to promote the progression of spermatogenesis. PMID: 18937323
    48. JAM-A dimerization facilitates formation of a complex with Afadin and PDZ-GEF2 that activates Rap1A, which regulates beta1 integrin levels and cell migration. PMID: 19176753
    49. phosphorylation of Rap1GAP by protein kinase A plays an important role in striatal neurons by modulating Rap1 actions PMID: 19218462
    50. These results suggest that Rap-1 is activated upon CTLA-4 ligation and mediates inhibitory signals through prevention of immunological synapse formation. PMID: 19490760

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Cell junction. Cytoplasm. Cytoplasm, perinuclear region. Early endosome. Late endosome.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 数据库链接：

    KEGG: mmu:109905

    STRING: 10090.ENSMUSP00000088174

    UniGene: Mm.101922