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  4. Recombinant Mouse Regulator of G-protein signaling 9 (Rgs9)

Recombinant Mouse Regulator of G-protein signaling 9 (Rgs9)

  • 货号：
    CSB-YP019662MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019662MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019662MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019662MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019662MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rgs9
  • Uniprot No.：
  • 别名：
    Rgs9; Regulator of G-protein signaling 9; RGS9
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-675
  • 氨基酸序列
    MTIRHQGQQY RPRMAFLQKI EALVKDMQNP ETGVRMHNQR VLVTSVPHAM TGGDVLQWIT QRLWISNLEA QNLGNFIVKY GYIYPLQDPK NLILKPDSSL YRFQTPYFWP TQQWPAEDTD YAIYLAKRNI KKKGILEEYE KENYDFLNKK INYKWDFVIM QAKEQYRTGK ERNKADRYAL DCQEKAYWLV HRSPPGMNNV LDYGLDRVTN PNEVKKQTVT AVRKEIMYYQ QALMRSTVKS SVSLGGIVKY SEQFSSNDAI MSGCLPSNPW ITDDTQFWDL NAKLVEIPTK MRVERWAFNF SELIRDPKGR QSFQYFLKKE FSGENLGFWE ACEDLKYGDQ SKVKEKAEEI YKLFLAPGAR RWINIDGKTM DITVKGLRHP HRYVLDAAQT HIYMLMKKDS YARYLKSPIY KEMLAKAIEP QETTKRSSTL PFMRRHLRSS PSPVILRQLE EEEKAREAAN TVDITQPGQH LAPSPHLAVY TGTCVPPSPS SPFSPSCRSP RKPFASPSRF IRRPSIAICP SPSRVALEGS SGLEPKGEAS WSGANSGPSV TENREPSADH SRPQPRAPPK ARAALSLGRF LRRGCLASPV FARLSPKCPS VSHGKVQPLG DMGQQLPRLK PKKVANFFQI KMEMPTDSGT CLMDSDDPRA GESGDQTTEK EVICPWESLA EGKAG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to GNAT1. Involved in phototransduction; key element in the recovery phase of visual transduction.
  • 基因功能参考文献：
    1. Association with the plasma membrane is sufficient for potentiating catalytic activity of RGS7 and RGS9-2 proteins. PMID: 26811338
    2. Data show that the regulator of G-protein signaling 9-2 (RGS9-2) in the nucleus accumbens (NAc) affects the expression of many genes known to be involved in nociception, analgesia, and antidepressant drug actions. PMID: 26305935
    3. role in sensory and mood related symptoms of neuropathic pain PMID: 25150149
    4. gene regulation in the Striatum of RGS9-deficient mice PMID: 24663062
    5. The complex of G protein regulator RGS9-2 and Gbeta(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum. PMID: 22932702
    6. The expression of RGS9 in two distinct populations of neural precursor cells demonstrates that RGS9 and its accessory proteins may play an important role in neuronal development. PMID: 21616123
    7. RGS9 knockout causes a short delay in light responses of ON-bipolar cells. PMID: 22096596
    8. These studies with humans, rats and mice implicate RGS9-2 as a factor in regulating body weight. PMID: 22132185
    9. RGS9-2 may play a crucial role in opiate-mediated analgesic mechanisms at the level of the spinal cord. PMID: 21741448
    10. In this study, we report an identification of Hsc70 (Heat shock cognate protein 70) as a critical mediator of RGS9-2 expression that is specifically recruited to the intrinsically disordered C-terminal domain of RGS9-2 following dissociation from R7BP. PMID: 20095651
    11. The localization of RGS9-2 immunoreactivity at the base or neck of spines along dendritic shafts suggests that they are mainly localized at inhibitory post-synaptic sites or extrasynaptic sites near the excitatory synapses. PMID: 19912469
    12. identification of protein kinase C isozymes responsible for phosphorylation at Ser475 PMID: 12499365
    13. study demonstrates that the long regulator of G-protein signaling 9 isoform contains a C-terminal domain that provides high-affinity interaction with its target G protein PMID: 12818172
    14. data demonstrate a functional interaction between RGS9-2 and D2 receptor signaling and the behavioral actions of psychostimulants and suggest that psychostimulant induction of RGS9-2 represents a compensatory adaptation that diminishes drug responsiveness PMID: 12818179
    15. opiate-induced changes in RGS9 levels contribute to the behavioral and neural plasticity associated with chronic opiate administration PMID: 14595021
    16. photoreceptors R9AP determines the stability of the RGS9.Gbeta5 complex, and therefore all three proteins, RGS9, Gbeta5 , and R9AP, are obligate members of the regulatory complex that speeds the rate at which transducin hydrolyzes GTP. PMID: 14625292
    17. In the mouse, R9AP is detectable predominantly in photoreceptors, but it is also weakly expressed in other areas of the central nervous system. It may target GTPase-activating proteins to specific membranous compartments. PMID: 14664818
    18. Tolerance that develops following an adequate dose of morphine is caused by the stabilization and retention of mu receptor-activated Galpha subunits by RGS9-2 proteins. PMID: 15632124
    19. Changes in RGS9-2 may be a key factor in the pathway leading from D2 dopamine receptors to the side effects from the treatment of psychoses & Parkinson's disease. PMID: 15728856
    20. RGS9-2 plays an essential role in opiate actions by negatively modulating mu-opioid receptor downstream signaling PMID: 17725581
    21. RGS9 knockout mice have decreased motor coordination on the rotarod and deficits in working memory but exhibit normal locomotor activity, anxiety-like behavior, cue and contextual fear conditioning, startle threshold, and pre-pulse inhibition. PMID: 18073128
    22. RGS9-2 possesses specific degradation determinants which target it for constitutive destruction by lysosomal cysteine proteases. PMID: 18094251
    23. versatility of RGS9-2 results from its ability to inactivate the G protein, transducin, regardless of its effector interactions, whereas RGS9-1 prefers the G protein-effector complex PMID: 19098104
    24. The new kinetic scheme and analysis explain how and why RGS9 concentration matters in rod phototransduction, and they provide a framework for understanding the molecular mechanisms that rate-limit deactivation in other G-protein systems. PMID: 19751658

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  • 亚细胞定位：
    [Isoform 1]: Membrane; Peripheral membrane protein. Note=Isoform 1 is targeted to the membrane via its interaction with RGS9BP.
  • 组织特异性：
    Isoform 1 is expressed in photoreceptor outer segments. Isoform 2 is expressed in brain striatum.
  • 数据库链接：

    KEGG: mmu:19739

    STRING: 10090.ENSMUSP00000020920

    UniGene: Mm.38548