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  4. Recombinant Mouse Ribosomal RNA small subunit methyltransferase NEP1 (Emg1)

Recombinant Mouse Ribosomal RNA small subunit methyltransferase NEP1 (Emg1)

  • 中文名称：
    小鼠Emg1重组蛋白
  • 货号：
    CSB-YP007636MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Emg1重组蛋白
  • 货号：
    CSB-EP007636MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Emg1重组蛋白
  • 货号：
    CSB-EP007636MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Emg1重组蛋白
  • 货号：
    CSB-BP007636MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Emg1重组蛋白
  • 货号：
    CSB-MP007636MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Emg1
  • Uniprot No.：
  • 别名：
    Emg1; C2f; Grcc2f; Ribosomal RNA small subunit methyltransferase NEP1; EC 2.1.1.-; 18S rRNA; pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-244
  • 氨基酸序列
    SAASGGFQP RERRFSVQEQ DWETTPPKKL RLGAGSKCGG RRLIVVLEGA SLETVKVGKT YELLNCDRHK SMLLKNGRDP GEVRPDITHQ SLLMLMDSPL NRAGLLQVYI HTQKNVLIEV NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD GPQKLLKVIK NPVSDHFPVG CMKIGTSFSV EDISDIRELV PSSDPVVFVV GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKVTTAFEEV WGVI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.
  • 基因功能参考文献：
    1. Data suggest that Essential for Mitotic Growth 1 (EMG1) deficiency caused mitotic arrest and a halt in cell growth. PMID: 25708872
    2. Data demonstrate that Emg1 is highly expressed during mouse embryonic development, and essential for mouse pre-implantation development. PMID: 20858271
  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase NEP1 family
  • 数据库链接：

    KEGG: mmu:14791

    STRING: 10090.ENSMUSP00000004379

    UniGene: Mm.6390