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  4. Recombinant Mouse Runt-related transcription factor 2 (Runx2)

Recombinant Mouse Runt-related transcription factor 2 (Runx2)

  • 中文名称：
    小鼠Runx2重组蛋白
  • 货号：
    CSB-YP020594MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Runx2重组蛋白
  • 货号：
    CSB-EP020594MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Runx2重组蛋白
  • 货号：
    CSB-EP020594MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Runx2重组蛋白
  • 货号：
    CSB-BP020594MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Runx2重组蛋白
  • 货号：
    CSB-MP020594MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Runx2
  • Uniprot No.：
  • 别名：
    Runx2; Aml3; Cbfa1; Osf2; Pebp2a; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-607
  • 氨基酸序列
    MLHSPHKQPQ NHKCGANFLQ EDCKKALAFK WLISAGHYQP PRPTESVSAL TTVHAGIFKA ASSIYNRGHK FYLEKKGGTM ASNSLFSAVT PCQQSFFWDP STSRRFSPPS SSLQPGKMSD VSPVVAAQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQEAAA AAAAAAAAAA AAAAAVPRLR PPHDNRTMVE IIADHPAELV RTDSPNFLCS VLPSHWRCNK TLPVAFKVVA LGEVPDGTVV TVMAGNDENY SAELRNASAV MKNQVARFND LRFVGRSGRG KSFTLTITVF TNPPQVATYH RAIKVTVDGP REPRRHRQKL DDSKPSLFSD RLSDLGRIPH PSMRVGVPPQ NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS SLSKKSQAGA SELGPFSDPR QFPSISSLTE SRFSNPRMHY PATFTYTPPV TSGMSLGMSA TTHYHTYLPP PYPGSSQSQS GPFQTSSTPY LYYGTSSASY QFPMVPGGDR SPSRMVPPCT TTSNGSTLLN PNLPNQNDGV DADGSHSSSP TVLNSSGRMD ESVWRPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
  • 基因功能参考文献：
    1. Findings suggest that inhibition of Runx2 in chondrocytes could at least partially rescue destabilization of the medial meniscus (DMM) surgery-induced osteoarthritis (OA)-like defects. PMID: 28539595
    2. This study reveals that RUNX2 is differentially expressed in ATII cells vs. fibroblasts in lung fibrosis, which contributes to profibrotic cell function. Cell-specific targeting of RUNX2 pathways may represent a therapeutic approach for IPF. PMID: 28986417
    3. we have identified novel interaction between p38 MAPK and Runx2 enhances Runx2 transactivity, thus promoting vascular smooth muscle cells calcification PMID: 29495001
    4. By using microRNA array, we identified a large number of microRNAs that showed different expression between wild-type Runx2 group and mutant groups. The aim of this study is to find out the effect of mmu-miR-1963, which was downregulated in all mutant Runx2 groups, on the ameloblast differentiation of LS8 cells PMID: 29233684
    5. Results indicate that Dlx5 and Runx2 are critical factors for the upregulated Osterix expression in shPP2A cells, which is considered to be important for the accelerated osteoblast differentiation in these cells. PMID: 29068100
    6. the expression of mouse Amtn gene in amelogenesis is mediated by Runx2/Cbfbeta complex. Runx2/Cbfbeta can bind to the two Runx2 binding motifs AACCACT (-1342/-1336) and AACCAAA (-98/-92) in the Amtn promoter and regulate Amtn gene expression. PMID: 29436627
    7. Mutant Runx2 regulates amelogenesis and osteogenesis through a miR-185-5p-Dlx2 axis. PMID: 29242628
    8. Puerarin may promote MC3T3E1 osteoblastlike cell viability and differentiation, which may be related to the downregulation of miR204 and the following activation of Runx2. PMID: 28901520
    9. mTOR/Raptor signaling is essential for bone formation in vivo through the regulation of Runx2 expression. PMID: 28686577
    10. these findings provide evidence that Cx43 and Runx2 functionally intersect in vivo to regulate cortical bone properties and affect osteoblast differentiation and proliferation PMID: 28419546
    11. miR-133a-5p targets the 3' UTR of RUNX2 mRNA to regulate the expression of RUNX2 negatively. PMID: 29359964
    12. muramyl dipeptide directly augmented osteoblast differentiation and bone-forming gene expression by Runx2 activation. PMID: 28337794
    13. Loss of Runx1 and Runx2 inhibits prostate cell motility. PMID: 27634876
    14. fetal administration of Entinostat/MS-275, a class I histone deacetylase (HDAC)-specific inhibitor, partially prevents delayed closure of cranial sutures in Runx2(+/-) mice strain of C57BL/6J by two mechanisms: 1) posttranslational acetylation of Runx2 protein, which stabilized the protein and activated its transcriptional activity; and 2) epigenetic regulation of Runx2 and other bone marker genes PMID: 28052439
    15. These data suggest that NaF promotes the osteoblastic differentiation of MC3T3-E1 cells through the Runx2/Osterix pathway and may be effective for the treatment of bone-related disorders. PMID: 28083749
    16. results suggest that Edil3 may stimulate osteoblast differentiation and matrix mineralization by increasing expression of Runx2 through alpha5beta1 integrin /ERK pathway PMID: 29182679
    17. Taken together, these results demonstrate that piperine enhance osteoblast differentiation through AMPK phosphorylation in MC3T3-E1 cells. PMID: 29203239
    18. Sphingosine 1 phosphate also up-regulated runt-related transcription factor 2 (Runx2) expression through S1PR2/RhoA/ROCK/Smad1/5/8 signaling. PMID: 27612439
    19. Nearly half of all DNase hypersensitivity sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 and/or the chromatin-related CTCF transcription factors. PMID: 29176792
    20. The findings suggest that the absence of uPA correlates with increased levels of Runx transcriptional regulators in a way that promotes inflammation-associated carcinogenesis. PMID: 29107070
    21. Fam50a might be a new positive regulator of ameloblast differentiation, increasing binding affinity of Runx2 to Ambn promoter by physically interacting with Runx2. PMID: 28574578
    22. These results suggest that Dietary methionine restriction (MR) alters bone remodeling and apposition. In MR mice, miR-31 in plasma and liver, and miR-133a, miR-335-5p, and miR-204 in the bone marrow was elevated. These miRNAs were shown previously to target and regulate Osterix and RUNX2 in bone, which could inhibit osteoblast differentiation and function. PMID: 27191548
    23. SIRT1 interacts with and promotes the transactivation potential of RUNX2. PMID: 28542607
    24. ABL potentiated the assembly and activation of the RUNX2-TAZ master transcription factor complex that is required for osteoblastogenesis, while antagonizing PPARgamma-mediated adipogenesis. PMID: 27797343
    25. urther investigation indicated that p205 may disturb the formation of Runx2/Ids complex and free more Runx2 to induce the differentiation process. Taken together, our findings demonstrated for the first time that p205 functions as an activator in osteoblast differentiation. PMID: 26965419
    26. identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog. PMID: 27916785
    27. overexpression of Fam3c caused reduction of Runx2 expression at both mRNA and protein levels. Fam3c was localized in the cytoplasm and it was not secreted outside the cell during osteoblast differentiation and therefore, may function intracellularly. Furthermore, Fam3c and TGF-beta1 were found to regulate each other reciprocally PMID: 27914282
    28. MAP kinase-dependent RUNX2 phosphorylation Is necessary for epigenetic modification of chromatin during osteoblast differentiation. PMID: 27514023
    29. suggest that RUNX2 and USAG-1 act in an antagonistic manner PMID: 27518316
    30. results provide evidence that WWP2 serves as a positive regulator of osteogenesis by augmenting RUNX2 transactivation in a non-proteolytic mono-ubiquitination manner. PMID: 28500134
    31. Klotho gene deficiency promotes high-fat diet-induced fibrosis in aortic valves, likely through the AMPKalpha-RUNX2 pathway. PMID: 27242197
    32. Runx2 might act downstream of C/EBPbeta to regulate the expression of Cox-2, Vegf and Mmp9 in the uterine stromal cells. PMID: 27147263
    33. results reveal a central mechanism underlying lineage-specific regulation by a Runx2/DICER/miRNAs cascade during osteogenic differentiation and bone development PMID: 27064596
    34. Perlecan is required for the chondrogenic and adipogenic differentiation from SMCs via its regulation of the Sox9 and PPARgamma gene expression, but not for osteogenic differentiation via Runx2 PMID: 27238423
    35. results indicate that the activity of the canonical Wnt/beta-catenin pathway in osteoblast is modulated by Runx2fa PMID: 27083516
    36. in murine bone marrow stromal cells undergoing osteogenic differentiation, TNF-alpha and it activated NF-kappaB pathway inhibit the expression of Runx2 gene, and suppress the Runx2-mediated osteogenic gene transcription via the 9.0 kb BSP promoter. PMID: 27401008
    37. results suggest that 1,25(OH)D induces the transdifferentiation of osteoblasts to adipocytes via Runx2 downregulation in osteoblasts. PMID: 27213351
    38. In a model of instability-induced osteoarthritis, Runx2 was overexpressed in articular chondrocytes and osteophyte-forming cells in LOX-1(+)/(+) mice and was significantly downregulated in articular chondrocytes and osteophyte-forming cells in LOX-1(-)/(-) mice. PMID: 26901593
    39. Runx2-modified adipose-derived stem cells promote tendon graft integration in anterior cruciate ligament reconstruction. PMID: 26743583
    40. FHL2 might interact with Runx2 to mediate mesenchymal cell differentiation at the early stages of tooth development and human dental pulp cell differentiation. PMID: 26759258
    41. our studies reveal a novel function for Runx2 in regulating mammary epithelial cell regenerative potential, possibly acting as a downstream target of WNT signalling. PMID: 26489514
    42. MED23 binds to RUNX2 and modulates its transcriptional activity. PMID: 27033977
    43. Hand1 downregulated Ihh gene expression in vitro by inhibiting Runx2 transactivation of the Ihh proximal promoter. PMID: 26918743
    44. increased intranuclear actin forces MSC into osteogenic lineage through controlling Runx2 activity; this process may be useful for clinical objectives of forming bone. PMID: 26140478
    45. We observed that tranilast increased mRNA expression of both Sox9 and Runx2 in differentiating ATDC5 chondrogenic progenitor cells. PMID: 26777999
    46. Results propose that Utx plays important roles in osteoblast differentiation by controlling the expressions of Runx2 and Osterix. PMID: 25920016
    47. Somatic transmission of Runx2 mRNAs in osteoblasts and osteosarcoma cells represents a versatile mechanism for translational rather than transcriptional induction. PMID: 26381402
    48. The data indicate a critical role of Runx2 in Smooth muscle cell (SMC) osteogenic phenotype change and mineral deposition in a mouse model of Arterial medial calcification (AMC). PMID: 25987250
    49. Gene expression of Tau, a neuronal phosphoprotein with important roles in neuronal biology and microtubule dynamics and assembly, was high in wild-type molars but severely reduced in transgenic(Col1a1-Runx2) molars. PMID: 25707508
    50. Runx2/miR-3960/miR-2861 positive feedback loop plays an important role in osteogenic transdifferentiation of vascular smooth muscle cells and contributes to vascular calcification. PMID: 26221600

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Found in thymus and testis, T-cell lines but not in B-cell lines. Isoform 2 is exclusively found in bone, particularly in osteoblasts; isoforms 3 and 4 are expressed in T-cell lines; isoforms 5, 6, 7, 8 and 9 can be found in osteoblasts and osteosarcoma c
  • 数据库链接：

    KEGG: mmu:12393

    STRING: 10090.ENSMUSP00000109201

    UniGene: Mm.391013