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  4. Recombinant Mouse S-arrestin (Sag)

Recombinant Mouse S-arrestin (Sag)

  • 中文名称：
    小鼠Sag重组蛋白
  • 货号：
    CSB-EP020669MOa0
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Sag
  • Uniprot No.：
  • 别名：
    Sag; S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    50.4 kDa
  • 表达区域：
    1-403aa
  • 氨基酸序列
    MAACGKTNKSHVIFKKVSRDKSVTIYLGKRDYVDHVSQVEPVDGVVLVDPELVKGKKVYVTLTCAFRYGQEDIDVMGLTFRRDLYFSRVQVYPPVGAMSVLTQLQESLLKKLGDNTYPFLLTFPDYLPCSVMLQPAPQDVGKSCGVDFEVKAFASDITDPEEDKIPKKSSVRLLIRKVQHAPPEMGPQPSAEASWQFFMSDKPLNLSVSLSKEIYFHGEPIPVTVTVTNNTDKVVKKIKVSVEQIANVVLYSSDYYVKPVASEETQEKVQPNSTLTKTLVLVPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVMGILVSYHIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESVQDENLVFEEFARQNLKDTGENTEGKKDEDAGQDE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO. May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.
  • 基因功能参考文献：
    1. We conclude that, in addition to their well-established roles in Meta II inactivation, Grk1 and Arr1 can modulate the kinetics of Meta III decay and rod dark adaptation in vivo. PMID: 27353443
    2. The G-protein coupled receptor, DRD4, requires ARR1 and ARR4 for desensitization and internalization. PMID: 26169958
    3. ARR4 modulates essential functions in high acuity vision and downstream cellular signaling pathways that are not fulfilled or substituted by the coexpression of ARR1, despite its high expression levels in all mouse cones. PMID: 26284544
    4. crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography PMID: 26200343
    5. Sag is essential for embryonic vasculogenesis and tumor angiogenesis. PMID: 24213570
    6. SAG knockdown caused the accumulation of proapoptotic Bax and SARM, imbalance of Bcl-2/Bax in the mitochondria, induction of cytosolic cytochrome c and activation of caspases, all of which led to disequilibrium between life and death of macrophages. PMID: 24786833
    7. tetrameric visual arrestin 1 is a biomarker for retinal function in diabetic mice, assessed by MRI PMID: 25351983
    8. The data suggest that monomeric arrestin-1 is cytotoxic and WT arrestin-1 protects rods by forming mixed oligomers with the mutant and/or competing with it for the binding to non-receptor partners. PMID: 24012956
    9. Findings suggest a role for Bardet-Biedl syndrome 5 (BBS5) in regulating light-dependent translocation of arrestin1 (Arr1). PMID: 23817741
    10. Visual arrestin interaction with clathrin adaptor AP-2 regulates photoreceptor survival in the vertebrate retina. PMID: 23690606
    11. the 139-loop stabilizes basal conformation of arrestin-1 and acts as a brake, preventing its binding to non-preferred forms of rhodopsin. PMID: 23476014
    12. a novel function of palmitoylation in shaping subcellular cAMP-PKA signaling in cardiomyocytes via modulating the recruitment of beta arrestin 2-PDE4D complexes to the agonist-stimulated beta(2)AR PMID: 22912718
    13. Photoresponse recovery rates of mice with arrestin-1 content in the outer segment, were measured. PMID: 21818392
    14. Physiological level of arrestin-1 expression in rods reflects the balance between short-term functional performance of photoreceptors and their long-term health. PMID: 21075174
    15. maintenance of low levels of the active monomer is the biological role of arrestin-1 self-association PMID: 21288033
    16. siRNA silencing induces radiosensitization by increasing ROS levels and blocking NF-kappaB activation PMID: 20638939
    17. study demonstrates a vital alternative function for Arr1 in the photoreceptor synapse and provides key insights into the potential molecular mechanisms of inherited retinal diseases, such as Oguchi disease and Arr1-associated retinitis pigmentosa. PMID: 20631167
    18. Prolonged illumination up-regulates retinal arrestin and Guca1a/b: a novel mechanism for light adaptation. PMID: 19332500

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  • 亚细胞定位：
    Cell projection, cilium, photoreceptor outer segment. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Arrestin family
  • 组织特异性：
    Detected in retina (at protein level).
  • 数据库链接：

    KEGG: mmu:20215

    STRING: 10090.ENSMUSP00000076948

    UniGene: Mm.1276