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  4. Recombinant Mouse SHC-transforming protein 1 (Shc1)

Recombinant Mouse SHC-transforming protein 1 (Shc1)

  • 货号：
    CSB-YP021253MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021253MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021253MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021253MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021253MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Shc1; Shc; ShcA; SHC-transforming protein 1; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-579
  • 氨基酸序列
    MDLLPPKPKY NPLRNESLSS LEEGASGSTP PEELPSPSAS SLGPILPPLP GDDSPTTLCS FFPRMSNLKL ANPAGGRLGP KGEPGKAAED GEGSAGAALR DSGLLPLLQD MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAARPTLPSA QMSSHLGATL PIGQHAAGDH EVRKQMLPPP PCPGRELFDD PSYVNIQNLD KARQAGGGAG PPNPSLNGSA PRDLFDMKPF EDALRVPPPP QSMSMAEQLQ GEPWFHGKLS RREAEALLQL NGDFLVREST TTPGQYVLTG LQSGQPKHLL LVDPEGVVRT KDHRFESVSH LISYHMDNHL PIISAGSELC LQQPVDRKV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.
  • 基因功能参考文献：
    1. Study in MMTV/MT6 and MMTV/NIC breast cancer mouse models show that ShcA phosphotyrosine motifs potentiate immune suppression by limiting signal transducer and activator of transcription (STAT)-1-driven anti-tumour immunity, while simultaneously increasing STAT3 immunosuppressive signals. PMID: 28276425
    2. Sirt3 modulates age-associated mitochondrial biology and function via lysine deacetylation of target proteins, and s show that its regulation depends on its nitration status and is benefited by the improved NAD(+)/NADH ratio in aged p66(Shc(-/-)) brain mitochondria. PMID: 29721150
    3. miR-200c might be responsible for muscle wasting and myotube loss, most probably via a p66Shc-dependent mechanism in a pathological disease such as Duchenne muscular dystrophy. PMID: 29636844
    4. Data show that 66-kDa Src homology 2 domain-containing protein (p66Shc) is acetylated under high glucose conditions and is deacetylated by Sirtuin1 lysine deacetylase (Sirt1) on lysine 81. PMID: 28137876
    5. a pivotal role for p66(shc) -induced vascular dysfunction in a common pathogenic cascade shared by noise-induced and age-related hearing loss, is reported. PMID: 27157635
    6. P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture PMID: 27385725
    7. The results indicate that Shc proteins should be considered as potential targets for developing interventions to mitigate weight gain on high-fat diet by stimulating energy expenditure. PMID: 29243276
    8. Hyperglycemia and elevated free fatty acids in the diabetic milieu recruit p66Shc to upregulate endothelial miR-34a via an oxidant-sensitive mechanism, which leads to endothelial dysfunction by targeting Sirt1. PMID: 27789474
    9. p66SHC-mediated oxidative stress and telomere shortening synergize in some tissues (including testes) to accelerate aging. PMID: 26968134
    10. Taken together, these data argue for a complex mechanism of PKCbeta-dependent regulation of p66 activation involving Ser(139) and a motif surrounding Ser(213). PMID: 27624939
    11. JNK1/2-dependent regulation of p66ShcS36 phosphorylation, is reported. PMID: 26868434
    12. Data show that the major mitochondrial partner of Shc adaptor protein p46Shc is the lipid oxidation enzyme 3-ketoacylCoA thiolase ACAA2, to which p46Shc binds directly and with a strong affinity. PMID: 27059956
    13. In mice and humans, reduced p66Shc levels protect from obesity, but not from ectopic fat accumulation, glucose intolerance and insulin resistance. PMID: 26122877
    14. p53-dependent augmentation of p66(Shc) expression and function represents a key signalling response contributing to beta cell apoptosis under conditions of lipotoxicity PMID: 25810038
    15. Results show that genetic deletion of p66(Shc) increased susceptibility to myocardial injury in response to short-term ischaemia and reperfusion in mice PMID: 25336219
    16. myocardial infarction transiently induced expression and phosphorylation of cardiac p66ShcA in mice PMID: 25680868
    17. Silencing of p66(Shc) restored insulin response via IRS-1/Akt/eNOS pathway. Its knockdown in endothelial cells from Ob/Ob mice lessened ROS production, FFA oxidation, and dysregulation of redox-sensitive pathways. PMID: 25150941
    18. These data identify multiple modes by which ShcA can fine-tune the development of early thymocytes, including a previously unappreciated ShcA-c-Abl axis that regulates thymocyte proliferation. PMID: 25691660
    19. PKCbeta2 inhibition protects mice from gut ischemia-reperfusion injury by suppressing the adaptor p66(Shc)-mediated oxidative stress and subsequent apoptosis. PMID: 24722289
    20. Endothelial function in cerebral arteries is heavily impaired by aging and is partly mediated by the p66Shc gene. PMID: 25012499
    21. ShcA interacts with crucial proteins and pathways that link Z-disk and costamere during heart development PMID: 25488665
    22. these data identify an important role for the adapter protein ShcA in later stages of thymic T cell development and in peripheral T cell-dependent events. PMID: 25595778
    23. CRIF1 knockdown partially induces endothelial activation via increased ROS production and phosphorylation of p66shc PMID: 24906005
    24. The regulation of p66shc-related antioxidative and antiapoptotic factors contributes to protection from ischemia-reperfusion injury. PMID: 24757420
    25. p66Shc is not a longevity protein. However, additional studies are needed to determine the extent to which Shc proteins may influence the onset and severity of specific age-related diseases. PMID: 24336818
    26. p66(Shc) plays a vital part in canonical Wnt signaling in the endothelium and mediates Wnt3a-stimulated endothelial oxidative stress and dysfunction. PMID: 25147340
    27. study identifies p66ShcA as one of the first prognostic biomarkers for the identification of more aggressive tumors with mesenchymal properties, regardless of molecular subtype. PMID: 25071152
    28. Higher sensitivity of the male kidney to AKI may be due to the testosterone-dependent increase in p66shc expression. PMID: 24632974
    29. This study thus identifies a previously unrecognized role for p66Shc in the regulation of PTP oxidation controlling growth factor-induced signaling and migration. PMID: 24378437
    30. Results identify p66Shc as a negative regulator of B-cell chemotaxis and suggest a role for this adaptor in the control of B-cell homing via CXCR4/5. PMID: 24556683
    31. unlike the other isoforms of Shc1, p66Shc appears to antagonize insulin and mTOR signaling, which limits glucose uptake and metabolism. PMID: 24550542
    32. study concludes hypercholesterolemia stimulates p66Shc expression in platelets, promoting platelet oxidative stress, hyperreactivity and hyperaggregation via p66Shc PMID: 24845561
    33. Deletion of p66Shc protects from experimental autoimmune encephalomyelitis without affecting immune response. PMID: 23766859
    34. autonomic neuropathy in the bone marrow impairs stem cell mobilization in diabetes with dysregulation of the life-span regulators p66Shc and Sirt1 PMID: 24270983
    35. p66shc has a role in renal toxicity of oleic acid PMID: 23988748
    36. Arg-II promotes mitochondrial dysfunction leading to VSMC senescence/apoptosis through complex positive crosstalk among S6K1-JNK, ERK, p66Shc, and p53, contributing to atherosclerotic vulnerability phenotype. PMID: 23832324
    37. Lung p66(Shc) attenuates epithelial proliferation while promoting both distal and proximal epithelial maturation. PMID: 24375794
    38. These findings identify p66Shc as a critical regulator of actin dynamics in mast cells. PMID: 24078705
    39. P66Shc represents a clear example of an antagonistic pleiotropic function, which generates both beneficial (thermoregulation control) and detrimental ROS generation) phenomena in an organism. PMID: 23849861
    40. Increased phosphorylation of p66shc is associated with renal oxidative stress and injury. PMID: 23328708
    41. Salvianolic acid A induced SIRT1 plays an anti-apoptotic role in concanavalin A induced hepatitis by inhibiting p66Shc expression. PMID: 23993977
    42. mice, p66Shc contributes to tumor initiation, in particular upon activation by carcinogens as well as when p53- mediated tumor suppression mechanisms defect PMID: 23092318
    43. oxidative stress activates a specific p53 transcriptional response, mediated by p44/p53 and p66, which regulates cellular senescence and aging. PMID: 23448364
    44. Shc1 scaffold therefore directs the temporal flow of signalling information after EGF stimulation PMID: 23846654
    45. Distinct phosphotyrosine-dependent functions of the ShcA adaptor protein are required for transforming growth factor beta (TGFbeta)-induced breast cancer cell migration, invasion, and metastasis PMID: 23277357
    46. Null mutations at the p66 and bradykinin 2 receptor loci induce divergent phenotypes in the diabetic kidney in mice. PMID: 23019230
    47. Protocatechuic acid treatment down-regulated p66shc expression and reduced I/R-induced lung injury in mice, reinforcing the role p66shc plays in apoptosis. PMID: 23117377
    48. Data show that hydrogen peroxide (H2O2) activates a protein kinase C beta/p66(shc)/NF-kappaB cascade and that p66(shc) is an essential mediator of the stimulating effects of H2O2 on apoptosis of osteoblastic cells as well as their ability to activate NF-kappaB. PMID: 20685851
    49. The results indicated that p66Shc does not significantly influence energy expenditure in 18mo mice at baseline or 2 mo of caloric restriction, although it may play a role in the energy expenditure response to very acute caloric restriction. PMID: 22588161
    50. Mitochondrial adaptor p66(Shc) is the key effector driving vascular hyperglycemic memory in experimental diabetes mellitus. PMID: 22693349

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  • 亚细胞定位：
    Cytoplasm.; [Isoform p47Shc]: Mitochondrion matrix.; [Isoform p66Shc]: Mitochondrion. Note=In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
  • 组织特异性：
    Widely expressed. Expressed in neural stem cells but absent in mature neurons.
  • 数据库链接：

    KEGG: mmu:20416

    STRING: 10090.ENSMUSP00000091940

    UniGene: Mm.86595