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  4. Recombinant Mouse SPARC (Sparc)

Recombinant Mouse SPARC (Sparc)

  • 货号：
    CSB-YP022486MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022486MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022486MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022486MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022486MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sparc; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-302
  • 氨基酸序列
    APQ QTEVAEEIVE EETVVEETGV PVGANPVQVE MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL VI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • 基因功能参考文献：
    1. It is a matricellular glycol that regulates cell proliferation, tissue repair, and tumorigenesis and a potential therapeutic target for conditions involving intestinal inflammation. PMID: 28582593
    2. Sparc levels in tendons are critical for proper collagen fibril maturation. PMID: 27586416
    3. miR-29 directly targeted SPARC, resulting in degradation of SPARC-encoding mRNA and reduction in the SPARC protein level. PMID: 29462611
    4. SPARC plays a crucial role in the proliferation and differentiation of C2C12 and may be involved in the link between the ECM remodeling and mitochondrial func PMID: 29420632
    5. SPARC is regulating the interplay between myeloid-derived suppressor cells and the extracellular matrix to drive the induction of epithelial-to-mesenchymal transition in tumor cells. PMID: 27681434
    6. The results demonstrate for the first time a functional role of the N-propeptide in regulating collagen fiber assembly and cell behavior and suggest that SPARC and the N-propeptide of collagen I have distinct activities in regulating collagen fiber assembly and fibroblast function. PMID: 28245286
    7. SPARC plays a key role in influencing the spatial organization of the anterior segment, potentially via modulation of collagen properties, while Hevin is not likely to be involved. PMID: 28122087
    8. An ADAMTS1 blocking antibody suppressed the SPARC-induced collagen I secretion, indicating that SPARC promoted collagen production directly through ADAMTS1 interaction. In conclusion, ADAMTS1 is an important mediator of SPARC-regulated cardiac aging. PMID: 27143554
    9. SPARC appears to be an important modulator of the actin cytoskeleton, implicating maintenance of muscular function PMID: 27908613
    10. resistivity measurements were taken on 22 mice, 11 wild-type and 11 sparc-/- (knock out for the protein SPARC: secreted protein acidic and rich in cysteine), bearing mammary carcinomas. PMID: 27099155
    11. SPARC isoforms, acting on Adipose stromal cells through distinct mechanisms, have an additive effect in inducing ASC migration. PMID: 26381424
    12. SPARC levels were not associated with efficacy in patients with MPC. This exploratory analysis does not support making treatment decisions regarding nab-paclitaxel plus gemcitabine or gemcitabine alone in MPC based on SPARC expression. PMID: 26169969
    13. SPARC is proposed to act as a critical regulator of transglutaminase activity on collagen I with implications for mechanical strength of tissues. PMID: 25827352
    14. Tumor-produced SPARC and VCAM1 are regulators of cancer extravasation. PMID: 25925867
    15. The results of this study indicated that the loss of p53 by deletion/mutation in the early stages of glioma formation may cooperate with the induction of SPARC to potentiate cancer cell survival and escape from immune surveillance. PMID: 24862407
    16. This work suggests that SPARC could affect anxiety-related behavior by modulating neuronal activity, and that depression-related behavior is dependent upon the adult expression of SPARC, which affects adult brain function. PMID: 22950524
    17. SPARC has a significant role in the development of concanavalin A -induced severe liver injury. PMID: 25410742
    18. SPARC acts as an important mediator of age-related cardiac inflammation by increasing the expression of macrophage M1 markers and decreasing M2 markers. PMID: 25877699
    19. SPARC inhibits growth factor-induced signaling in both INS-1 beta cells and primary mouse islets, and inhibits IGF-1-induced proliferation of INS-1 beta cells. PMID: 25204658
    20. Age and SPARC change the extracellular matrix composition of the left ventricle. PMID: 24783223
    21. findings suggest that SPARC plays a crucial role in the regulation of early B lymphopoiesis. PMID: 24598056
    22. SPARC-deficient mice display higher resistance to serial treatment with the chemotherapeutic agent 5-fluorouracil (5-FU). PMID: 24833352
    23. effects of age and the expression of SPARC on extracellular matrix production by cardiac fibroblasts PMID: 24223185
    24. SPARC KO mice had less inflammation. PMID: 24204877
    25. These results demonstrate that there is a delicate temporal regulation of SPARC and that disturbances in this may have an adverse effect on muscle regeneration. PMID: 23670848
    26. These findings suggest that SPARC plays a critical role in the maintenance of miMEFs without loss of cell number and might be a key component for supporting the culture of human PSCs. PMID: 23661086
    27. Loss of SPARC not only upregulates atrogin 1 expression but also enhances transforming growth factor (TGF)beta signaling, which may in turn cause muscle atrophy. PMID: 23424163
    28. data suggest that SPARC plays a significant role in liver fibrogenesis. Interventions to inhibit SPARC expression are suggested as promising approaches for liver fibrosis treatment PMID: 23408952
    29. new insight into the biological functions of SPARC PMID: 23349702
    30. These findings suggest that exercise stimulates SPARC secretion from muscle tissues and that SPARC inhibits colon tumorigenesis by increasing apoptosis. PMID: 22851666
    31. SPARC modulates intraocular pressure regulation in the eye, and also correlates with trabecular meshwork morphology. PMID: 23422826
    32. Using a chemical carcinogenesis model in Sparc-deficient mice and their wild-type littermates, we found that loss of SPARC accelerated the development of urothelial preneoplasia, neoplasia, and metastasis and was associated with decreased survival. PMID: 23321672
    33. This sdtudy demonistrated that SPARC regulates microgliosis and functional recovery following cortical ischemia and brain injury. PMID: 23467362
    34. SPARC and SC1 may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
    35. SPARC-null mice develop region-specific, age-dependent hypersensitivity to cold, icilin, and capsaicin; axial discomfort; motor impairment; and reduced physical function. PMID: 22414871
    36. SPARC suppresses angiogenesis of gastric cancer by down-regulating the expression of VEGF and MMP-7 PMID: 22957090
    37. Stromal SPARC contributes to the detrimental fibrotic changes associated with myeloproliferation whereas its deficiency favors myeloid cell expansion. PMID: 22955913
    38. SPARC promotes the development of erythroid progenitors, but does not affect terminal erythroid differentiation. PMID: 22687753
    39. The Sparc(-/-) mice had reduced survival and fewer well-defined abdominal lesions compared with WT controls after IP injection, whereas no differences were observed in survival or abdominal lesions between Sparc(-/-) and WT mice after OT injection. PMID: 22003835
    40. Osteonectin may suppress prostate cancer pathogenesis. PMID: 22525512
    41. aberrant TGFbeta1-activation in the absence of host SPARC contributes significantly to tumor progression and SPARC, by controlling ECM deposition and maturation, can regulate TGFbeta availability and activation PMID: 22348081
    42. SPARC influences the shuttling of Pax6 via the TGF-beta/Smad signaling pathway. PMID: 22539874
    43. follicular dendritic cell networking toward Th17 responses PMID: 21962567
    44. Data indicate that intracellular SPARC-positive vesicles did not undergo visible degradation. PMID: 21949685
    45. SPARC and connective tissue growth factor appeared to be involved in the same biological pathway PMID: 21978691
    46. DNA methylation down-regulates SPARC expression in chronic low back pain (LBP) in pre-clinical models and in patients with chronic LBP. PMID: 21867537
    47. SPARC regulates collagen in the heart by modulating procollagen processing and interactions with fibroblast cell surfaces. PMID: 21666116
    48. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
    49. SPARC deletion preserves left ventricular function at day 3 post-myocardial infarction but may be detrimental for the long-term response due to impaired fibroblast activation. PMID: 21602472
    50. SPARC promotes pericyte migration by diminishing TGF-beta activity and identify a novel function for endoglin in controlling pericyte behavior. PMID: 21708981

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
  • 蛋白家族：
    SPARC family
  • 数据库链接：

    KEGG: mmu:20692

    STRING: 10090.ENSMUSP00000018737

    UniGene: Mm.291442