Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse SUMO-conjugating enzyme UBC9 (Ube2i)

Recombinant Mouse SUMO-conjugating enzyme UBC9 (Ube2i)

  • 货号：
    CSB-YP025457MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025457MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025457MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025457MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025457MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ube2i; Ubc9; Ubce2i; Ubce9; SUMO-conjugating enzyme UBC9; EC 2.3.2.-; RING-type E3 SUMO transferase UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; mUBC9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-158
  • 氨基酸序列
    SGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
  • 基因功能参考文献：
    1. These findings suggest that the UBC9/PML/RNF4 axis plays a critical role as an important SUMO pathway in cardiac fibrosis. Modulating the protein levels of the pathway provides an attractive therapeutic target for the treatment of cardiac fibrosis and heart failure. PMID: 28143738
    2. this study shows that loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery PMID: 28314856
    3. Increased UBC9-mediated SUMOylation is sufficient to upregulate cardiac autophagy. UBC9 overexpression reduced aggregate formation, decreased fibrosis, reduced hypertrophy, and improved cardiac function and survival. PMID: 27142163
    4. Synaptic trapping of Ubc9 through a PKC phosphorylation-dependent increase of Ubc9 recognition to phosphorylated substrates and consequently leads to the modulation of synaptic sumoylation. PMID: 25311713
    5. UBC9 has a role in promoting proliferation and migration of fibroblast-like synoviocytes in rheumatoid arthritis PMID: 24531852
    6. the sole SUMO E2 enzyme Ubc9 plays a critical role in reprogramming fibroblasts to iPS cells and maintaining ESC pluripotency. PMID: 24706591
    7. Data indicate that knockdown of ubiquitin-conjugating enzyme 9 (UBC9) by small interfering RNA caused significant accumulations of aggregated protein. PMID: 25097219
    8. Ubc9 negatively regulates osteoblastic differentiation induced by BMP. PMID: 22366399
    9. expressed Ubc9 at modestly increased levels showed robust resistance to brain ischemia compared to wild type mice PMID: 22016779
    10. Ubiquitous loss of sumoylation due to loss of UBC9 led to immediate fatal failure of intestinal function. Sumoylation was required for nucleus positioning and polarized organization of actin in the enterocytes. PMID: 20951138
    11. FOXL2 interacts with PIAS1 and UBC9, and sumoylated in both human and mouse PMID: 20209145
    12. The temporal profile of UBC9 induction and its ability to affect C/EBPdelta mRNA induction support a role for this protein during early adipogenesis PMID: 20881252
    13. Knockdown of the SUMO-conjugating enzyme, Ubc9, severely compromises C2C12 muscle cell terminal differentiation. However, it does not affect the expression, the localization and the activation of MyoD and myogenin. PMID: 16631162
    14. Noncovalent interaction between Ubc9 and SUMO promotes SUMO chain formation. PMID: 17491593
    15. Transcriptionally quiescent, fully grown, GV-intact oocytes have larger UBE2I-containing bodies than transcriptionally active, meiotically incompetent, growing oocytes. PMID: 18703419
    16. Taken together, these observations suggest that Ubc9 functions as a transcriptional repressor of IRF-1 by inducing sumoylation, and that this effect may be required for the physiological activity of IRF-1. PMID: 18955028

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 组织特异性：
    Present in spleen, kidney, lung, brain, heart and testis (at protein level).
  • 数据库链接：

    KEGG: mmu:22196

    STRING: 10090.ENSMUSP00000055714

    UniGene: Mm.240044