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  4. Recombinant Mouse Securin (Pttg1)

Recombinant Mouse Securin (Pttg1)

  • 货号：
    CSB-YP887426MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP887426MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP887426MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP887426MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP887426MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pttg1; Pttg; Securin; Pituitary tumor-transforming gene 1 protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-199
  • 氨基酸序列
    ATLIFVDKD NEEPGRRLAS KDGLKLGTGV KALDGKLQVS TPRVGKVFNA PAVPKASRKA LGTVNRVAEK PMKTGKPLQP KQPTLTGKKI TEKSTKTQSS VPAPDDAYPE IEKFFPFNPL DFESFDLPEE HQISLLPLNG VPLMTLNEER GLEKLLHLGP PSPLKTPFLS WESDPLYSPP SALSTLDVEL PPVCYDADI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 基因功能参考文献：
    1. The findings show that maternal aging compromises the oocyte SAC-APC/C axis leading to a decrease in securin that ultimately causes sister chromatid cohesion loss. PMID: 28516917
    2. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
    3. High PTTG1 expression is associated with pituitary tumor development. PMID: 27524416
    4. PTTG is functionally required for hepatic fibrosis progression in an animal model of chronic liver injury, and thus a potential treatment target. PMID: 25936962
    5. RasGrf1 is an important upstream component of signal transduction pathways regulating Pttg1 expression and controlling beta cell development and physiological responses. PMID: 25421944
    6. these results implicate Pttg1 in the regulation of homotypic spacing between specific types of retinal neurons. PMID: 24927528
    7. Pttg1/securin has roles in branching morphogenesis of the mammary gland and suppression of mammary tumorigenesis PMID: 24395789
    8. data reveal that up to 9 months of age, Pttg1(-/-) retinas have a healthy morphology and normal photoreceptor function PMID: 21203837
    9. Pituitary tumor transforming gene-null male mice exhibit impaired pancreatic beta cell proliferation and diabetes. PMID: 12626748
    10. homologue disjunction in mouse oocytes is dependent on proteolysis of the separase inhibitor securin and cyclin B1 PMID: 14593421
    11. Physiological functions of PTTG are revealed by PTTG-null mice who exhibit a variety of cell growth abnormalities including diabetes mellitus secondary to defective beta-cell proliferation. Review. PMID: 15281346
    12. Securin phosphorylation act redundantly to prevent sister chromatid separation. PMID: 16030258
    13. results suggest that Rae1 and Nup98 are temporal regulators of APC(Cdh1) that maintain euploidy by preventing unscheduled degradation of securin PMID: 16355229
    14. results suggest that the proteolysis of securin is dependent on ubiquitin-proteasome pathway and is necessary for the degradation of Rec8 during meiotic metaphase-to-anaphase transitions in oocytes PMID: 16720305
    15. PTTG1 and its binding protein PTTG binding factor are expressed in pituitary FS TtT/GF cells. PMID: 16959877
    16. PTTG1, an oncogene, is regulated by the liganded thyroid hormone receptor beta PMID: 17039256
    17. Deregulation of Pttg1 expression is attributable to a cysteine-249-tryptophan substitution in the extracellular domain of crumbs homolog 1 (Crb1). PMID: 17234588
    18. Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit PMID: 17325031
    19. Pttg deletion reduces bone marrow stem cell proliferation, renders cells more sensitive to hypoxia, and enhances senescent features, thus pointing to a role for Pttg in the maintenance and proliferation of the cells PMID: 17626243
    20. In mouse pituitary tumor cell lines, PTTG was localized in the Golgi apparatus and vesicles. PMID: 17652795
    21. Pituitary tumor-transforming gene (Pttg) deletion results in pituitary hypoplasia, low pituitary cell proliferation rates, and rescue of pituitary tumor development in Rb(+/-) mice. PMID: 17975001
    22. Data show that in mouse oocytes, excess securin causes stabilization of cyclin B and precocious entry into M-phase. PMID: 18364698
    23. GPC1 regulates EC cell cycle progression at least partially by modulating APC/C-mediated degradation of mitotic cyclins and securin PMID: 18417614
    24. Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggs PMID: 18639540
    25. Beta-cell apoptosis and senescence contribute to the diminished beta-cell mass in PTTG(-/-) mice, likely secondary to DNA damage. PMID: 19213844
    26. PTTG1 and DLK1 are involved in cell differentiation and transformation. PMID: 19477929

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Securin family
  • 数据库链接：

    KEGG: mmu:30939

    STRING: 10090.ENSMUSP00000020687

    UniGene: Mm.6856