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  4. Recombinant Mouse Serine/threonine-protein kinase Nek2 (Nek2)

Recombinant Mouse Serine/threonine-protein kinase Nek2 (Nek2)

  • 中文名称：
    小鼠Nek2重组蛋白
  • 货号：
    CSB-YP015699MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nek2重组蛋白
  • 货号：
    CSB-EP015699MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nek2重组蛋白
  • 货号：
    CSB-EP015699MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nek2重组蛋白
  • 货号：
    CSB-BP015699MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nek2重组蛋白
  • 货号：
    CSB-MP015699MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nek2
  • Uniprot No.：
  • 别名：
    Nek2; Serine/threonine-protein kinase Nek2; EC 2.7.11.1; Never in mitosis A-related kinase 2; NimA-related protein kinase 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-443
  • 氨基酸序列
    MPSRVEDYEV LHSIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEVEKQ MLVSEVNLLR ELKHPNIVSY YDRIIDRTNT TLYIVMEYCE GGDLASVISK GTKDRQYLEE EFVLRVMTQL TLALKECHRR SDGGHTVLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQMSC LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLPDSS PVLSELKLKE RQLQDREQAL RAREDILEQK ERELCIRERL AEDKLARAES LMKNYSLLKE HRLLCLAGGP ELDLPSSAMK KKVHFHGESK ENTARSENSE SYLAKSKCRD LKKRLHAAQL RAQALADIEK NYQLKSRQIL GMR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction.
  • 基因功能参考文献：
    1. Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A PMID: 26220856
    2. The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis. PMID: 26320076
    3. Nek2 transgenic mice develop spontaneous germinal centers and exhibit an enhanced T cell dependent immune response our data demonstrates a novel role for Nek2 in regulating B cell development and the immune response PMID: 25485281
    4. these data indicate that Nek2 has a pivotal role in breast cancer growth at primary and secondary sites, and thus may be an attractive and novel therapeutic target for this disease. PMID: 23708664
    5. CIP2A strongly interacts with NEK2 during G2/M phase, thereby enhancing NEK2 kinase activity to facilitate centrosome separation in a PP1- and PP2A-independent manner. PMID: 24214971
    6. Nek2 and its substrate, centrobin/Nip2, are required for proper meiotic spindle formation of the mouse oocytes PMID: 20569513
    7. Data show that NEK2 was identified as a tumor-associated antigen. PMID: 20718755
    8. Nek2 may be a mitotic regulator that is involved in diverse cell cycle events. PMID: 11785960
    9. Tcp10 promoter-directed expression of the Nek2 gene in mouse meiotic spermatocytes. PMID: 11911479
    10. The MAPK pathway triggers activation of Nek2 during chromosome condensation in mouse spermatocytes. PMID: 11923207
    11. Functional interaction between HMGA2 and Nek2 plays a crucial role in the correct process of chromatin condensation in meiosis PMID: 14668482
    12. These results propose a possible involvement of Nek2 in biological processes of the Golgi body, perhaps in relation to the inheritance of Golgi during mitosis or to cell cycle stage-specific regulation of exocytosis. PMID: 14697346
    13. importance of Nek2 for proper chromosome segregation in embryonic mitoses. PMID: 15479717
    14. The phenotypic similarities of the Nip2/centrobin- and Nek2-suppressed embryos suggest that Nip2/centrobin may be a substrate of Nek2 that is required for mitotic spindle assembly in early mouse embryos. PMID: 19117032

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Most abundantly expressed in testis. Low levels found in mid-gestation embryo, ovary, placenta, intestine, thymus and skin. Within the testis, expression restricted to germ cells with highest levels detected in spermatocytes at pachytene and diplotene sta
  • 数据库链接：

    KEGG: mmu:18005

    STRING: 10090.ENSMUSP00000027931

    UniGene: Mm.33773