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  4. Recombinant Mouse Serine/threonine-protein kinase STK11 (Stk11)

Recombinant Mouse Serine/threonine-protein kinase STK11 (Stk11)

  • 中文名称：
    小鼠Stk11重组蛋白
  • 货号：
    CSB-BP893402MO
  • 规格：
    ￥3168
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Stk11; Lkb1; Serine/threonine-protein kinase STK11; EC 2.7.11.1; Liver kinase B1 homolog; LKB1; mLKB1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    Baculovirus
  • 分子量：
    52.9 kDa
  • 表达区域：
    1-433aa
  • 氨基酸序列
    MDVADPEPLGLFSEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHRNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFRQLIDGLEYLHSQGIVHKDIKPGNLLLTTNGTLKISDLGVAEALHPFAVDDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGRGDFTIPCDCGPPLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAEALVPIPPSPDTKDRWRSMTVVPYLEDLHGRAEEEEEEDLFDIEDGIIYTQDFTVPGQVLEEEVGQNGQSHSLPKAVCVNGTEPQLSSKVKPEGRPGTANPARKVCSSNKIRRLSAC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.; Has a role in spermiogenesis.
  • 基因功能参考文献：
    1. LKB1 induces the browning of white adipocytes, in addition to promoting lipid metabolism PMID: 30296566
    2. Data show that that the lineage switching of KRAS+ lung adenocarcinomas (ADC) to squamous cell carcinoma (SCC) through deletion of liver kinase B1 Lkb1 (Stk11). PMID: 28387316
    3. The role of Stk11 in chondrocyte. PMID: 30107230
    4. these data argue for a more complex role for Stk11 mutations in Peutz-Jeghers syndrome development, with deregulated inflammatory responses by LKB1 mutant immune cells, in addition to epithelial and stromal tissues, reinforcing tumor inflammation and chronic STAT3 activation to drive polyp growth. PMID: 30049881
    5. Deletion of Lkb1 results in a cytoplasm to nuclear translocation of CRTC3 in brown adipocytes, where it recruits C/EBPbeta to enhance Ucp1 transcription. PMID: 27461402
    6. The Stk11knockout mice develop prostatic hyperplasia with bladder outlet obstruction, most likely because of stromal expansion. PMID: 28289208
    7. Regulatory T cells lacking Lkb1 have defective mitochondria, compromised OXPHOS, depleted cellular ATP, and altered cellular metabolism pathways that compromise their survival and function. PMID: 29109251
    8. Identification of a new transcript variant Stk11N which is generated through alternative splicing. The new variant was found to have differential and tissue specific expression at Postnatal-7 and adult stages of mouse. PMID: 29777910
    9. This study supports different roles of Lkb1 isoforms in spermatogenesis with Lkb1L directing spermatogonial stem/progenitor cell maintenance, and Lkb1L and Lkb1S coordinately regulating spermatid differentiation. PMID: 29022902
    10. Metabolomics analysis suggests that Lkb1 mutant kidneys require glutamine and glutathione metabolism. PMID: 29483507
    11. These results demonstrate that Lkb1 plays an important role in maintaining body weight, liver and adipose tissue function, blood glucose homeostasis and survival in adult mice. PMID: 27824128
    12. The metabolic effects were associated with activation of the SIRT1-LKB1-AMPK signalling pathway in adipose tissue and liver. PMID: 28493443
    13. Treg cells use LKB1 signalling to coordinate their metabolic and immunological homeostasis and to prevent apoptotic and functional exhaustion, thereby orchestrating the balance between immunity and tolerance PMID: 28847007
    14. renal proximal tubule cell CB1R contributes to the pathogenesis of obesity-induced renal lipotoxicity and nephropathy by regulating the liver kinase B1/AMP-activated protein kinase signaling pathway PMID: 28860163
    15. Results reveal an important role for Lkb1 in regulating cerebellar cortical size and foliation in a Hedgehog-independent manner. PMID: 28974424
    16. beige adipocyte renaissance was governed by liver kinase b1 and histone deacetylase 4 in white adipocytes. PMID: 28882900
    17. miR-17-92-dependent tuning of LKB1 levels regulates both the metabolic potential of Myc+ lymphomas and tumor growth in vivo. PMID: 27498867
    18. Findings indicate that the energy-sensing LKB1-AMPK pathway regulates IGF1 secretion in mouse primary hepatocytes, which in turn regulates activation of the IGF1R-PKB pathway. PMID: 28500773
    19. These data suggest that nutrient availability dictates the mode of division and that LKB1-AMPK mediates this nutrient-driven effect on intestinal epithelial stem cell proliferation. PMID: 28766983
    20. this study identified the molecular mechanism of increased angiogenesis and tumor growth with LKB1 deficiency PMID: 28346429
    21. These results suggest that although physiologic LKB1 expression exerts a potent pro-survival effect in lymphocytes, LKB1 inactivation nonetheless facilitates transformation of B, but not T, lymphocytes. PMID: 28435024
    22. These results suggest that the LKB1-AMPK-FoxO1 signaling pathway is a critical mediator of the antioxidant properties of H2, further supporting the idea that H2 acts as a signaling molecule to serve various physiological functions. PMID: 28743498
    23. Lkb1 activates the Notch signaling pathway, which subsequently increases Pax7 expression and promotes self-renewal and proliferation while inhibiting differentiation. Mechanistic studies reveal that Lkb1 regulates Notch activation through AMPK-mTOR pathway in myoblasts. PMID: 27131604
    24. mitochondrial dysfunction triggers LKB1-mediated AMPK activation, which stimulates Sirt2 phosphorylation, leading to activation of mTOR-RAPTOR and Glut1-mediated glucose uptake. PMID: 27793977
    25. these data demonstrated that LKB1/AMPK signaling pathway activation improved the survival of diabetic mice complicated with endotoxemia. Thus, LKB1/AMPK signaling pathway may serve as a potentially useful therapeutic target for severe infection in diabetic patients. PMID: 28628912
    26. Data show that the serine/threonine kinase LKB1 regulates mitochondrial calcium uniporter (MCU)-expression, mitochondria-dependent Ca2+ clearance, and thereby, presynaptic release properties. PMID: 27429220
    27. LKB1 deficiency in LKB1(Pax2) CKO mice disrupted hair cell planar polarity during embryonic development. The results suggest that LKB1 is required in planar cell polarity formation in cochlear hair cells in mice. PMID: 27896618
    28. silencing of the LKB1 tumor suppressor affects the delamination of pre-migratory cephalic neural crest cells from the neural primordium as well as their polarization and survival, thus resulting in severe facial and brain defects PMID: 27527806
    29. LKB1 complex proteins have a role in myofilament function and myocellular "energy" sensing in the heart PMID: 26971467
    30. identification of a network linking metabolic and epigenetic alterations that is central to oncogenic transformation downstream of the liver kinase B1 (LKB1, also known as STK11) tumour suppressor, an integrator of nutrient availability, metabolism and growth PMID: 27799657
    31. The lack of LKB1 in skeletal muscle leads to an increased inflammatory state. PMID: 26796753
    32. Partial deficiency of cardiac LKB1 promotes the adverse effects of a high-fat, high-sucrose diet on the myocardium, leading to worsening of diastolic function and the de novo appearance of systolic dysfunction. PMID: 26722122
    33. Rapid activation of AMPK was detected after exposure of cortical neuronal cultures to zinc, which was induced by LKB1 activation but not increased intracellular AMP levels or CaMKKbeta activation. PMID: 26856538
    34. LKB1 deletion causes early changes in atrial channel expression and electrophysiology prior to atrial fibrillation. PMID: 26378152
    35. LKB1 promotes maturation of bile ducts during biliary morphogenesis.LKB1 and Notch share a common genetic program in the liver, and regulate bile duct morphogenesis. PMID: 26689699
    36. STK11/LKB1-inactivating mutations were associated with reduced expression of PD-1 ligand PD-L1 in mouse and patient tumors as well as in tumor-derived cell lines. PMID: 26833127
    37. activation of AKT and mTORC1, the double knockout of PTEN and LKB1 contributes to distinct cell-specific aspects of tumor development and progression. PMID: 26329580
    38. we show that loss of LKB1 in renal tubular epithelial cells has an important role in kidney disease development by influencing intracellular metabolism PMID: 26054542
    39. The LKB1 K178R SUMO mutant had defective AMPK signaling and mitochondrial function, inducing death in energy-deprived cells. PMID: 26212320
    40. data indicates that LKB1 is required for the development and maintenance of stereocilia in the inner ear. PMID: 26274331
    41. LKB1 mediates CDKN1A degradation in response to ultraviolet DNA damage. PMID: 25329316
    42. There was an inverse relationship between DEC1 expression and AMPK activity. Our results suggest that DEC1 negatively regulates AMPK activity via LKB1 PMID: 26498531
    43. data reveal cell intrinsic and surprising cell extrinsic roles for LKB1 in B cells that control T follicular helper-cell differentiation and germinal center formation, and place LKB1 as a central regulator of T-cell-dependent humoral immunity PMID: 25916856
    44. LKB1 KO mice develop spontaneous atrial fibrillation from sinus rhythm and progress into persistent AF, replicating the human AF disease process. PMID: 25773299
    45. heterozygous loss of Cdh1 exacerbates the number of extrusions per blastocyst, suggesting that LKB1 has a role in regulating adherens junctions in order to prevent extrusion in epithelia. PMID: 25588837
    46. LKB1 is essential for mitochondrial maintenance and negatively regulates a distal step of insulin secretion. PMID: 26139601
    47. Lkb1-deficient lung adenocarcinoma progressively transdifferentiates into squamous cell carcinoma. PMID: 24531128
    48. loss of LKB1 expression be considered a marker for metabolic dysfunction given its role in regulating AMPK and mTOR function. PMID: 25436981
    49. Sik1, Sik2, and Sik3 play a key role as gluconeogenesis suppressors downstream of LKB1 in the liver. PMID: 25088745
    50. Phosphorylation of LKB1 establishes Schwann cell polarity to initiate myelin extent. PMID: 25255972

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Membrane. Mitochondrion.; [Isoform 2]: Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, LKB1 subfamily
  • 组织特异性：
    [Isoform 1]: Widely expressed.; [Isoform 2]: Predominantly expressed in testis (at protein level).; [Isoform 3]: Expressed in adult brain and liver and absent from tissues derived from postnatal day 7.
  • 数据库链接：

    KEGG: mmu:20869

    STRING: 10090.ENSMUSP00000003152

    UniGene: Mm.44231