Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (Ppp1cc)

Recombinant Mouse Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (Ppp1cc)

  • 货号：
    CSB-YP018505MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018505MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018505MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018505MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018505MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp1cc; Serine/threonine-protein phosphatase PP1-gamma catalytic subunit; PP-1G; EC 3.1.3.16; Protein phosphatase 1C catalytic subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-323
  • 氨基酸序列
    ADIDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E.; Required for normal male fertility.
  • 基因功能参考文献：
    1. These findings provide novel evidence for a role of miRNAs in memory formation and suggest the implication of PP1 in miRNAs processing in the adult brain. PMID: 27558292
    2. The interaction between PPP1CC2 and AKAP4 in human spermatozoa. PMID: 27913189
    3. DNA modification methylase inhibitor 5-aza-2'-deoxycytidine restrains the expression of PP1gamma which is related to learning and memory in mice. PMID: 27665473
    4. PP1 directly interacts with IRF3 and dephosphorylates IRF3 at Ser385 and Ser396, resulting in the suppression of TLR- and RLR-triggered IFN-beta production. PMID: 25239187
    5. The endogenous Ppp1cc promoter normally functions in the testis to maintain a sufficient level of PPP1CC2 expression for normal spermatogenesis to occur. PMID: 23082183
    6. In mouse testis, PPP1CC2 can form a complex with TSSK1 mediated by the direct interaction of each with the kinase substrate protein TSKS. Interaction between PPP1CC2 and TSKS is mediated through an RVxF docking motif on the TSKS surface. PMID: 24088291
    7. Spermatogenic defects observed in the global Ppp1cc knockout mice and in mice expressing low levels of PPP1CC2 in testis are due to compromised functions of PPP1CC2 in meiotic and postmeiotic germ cells. PMID: 24089200
    8. PP1cgamma mutant sperm are unable to support development to the blastocyst stage, resulting in arrested development either before or just after compaction. PMID: 12606345
    9. The present study focused on TGF-beta-modulation of paxillin and the serine/threonine protein phosphatase PP-1, and the impact on cellular motility. PMID: 21965722
    10. Results identify protein phosphatase 1 (PP1) as regulator of period and light-induced resetting of the mammalian circadian clock. PMID: 21712997
    11. Present results demonisterated that not only phosphorylation but also dephosphorylation is a major mechanism involved in learning and memory. Therefore, inhibition of hippocampal phosphatase activity might improve learning and memory. PMID: 20299813
    12. glutamate receptors type 1a, 5a, and 5b bind to protein phosphatase 1C PMID: 14519764
    13. there is a protein phosphatase-1gamma1 isoform selectivity determinant in dendritic spine-associated neurabin PMID: 15016827
    14. the interaction between Spz1 and PP1cgamma2 may be required for proper regulation of spermatogenesis and fertility in males PMID: 15226296
    15. PP1gamma2 is involved in sperm tail morphogenesis. PMID: 17301292
    16. Ppp1cc was identified in the study. PMID: 17683050
    17. The in vivo consequences of disrupting the G(L)-phosphorylase a interaction, using a mouse model containing a Tyr284Phe substitution in the phosphorylase a-binding region of the hepatic glycogen-associated protein phosphatase-1 protein, is reported. PMID: 19275933
    18. Ikaros is controlled by the CK2 and PP1 pathways and that a balance between these two signal transduction pathways is essential for normal cellular function and for the prevention of malignant transformation. PMID: 19282287
    19. Data show a reciprocal relationship between protein phosphatase isoform PP1gamma2 and its regulator PPP1R11, and suggest that complex formation between them in testis may prevent proteolysis of PPP1R11 and germ cell apoptosis. PMID: 19300506
    20. Dab2 stabilizes Axin and attenuates Wnt/beta-catenin signaling by preventing PP1 from binding Axin PMID: 19581931

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Nucleus. Cleavage furrow. Nucleus, nucleolus. Nucleus, nucleoplasm. Chromosome, centromere, kinetochore. Nucleus speckle. Midbody. Mitochondrion. Cytoplasm, cytoskeleton, microtubule organizing center.
  • 蛋白家族：
    PPP phosphatase family, PP-1 subfamily
  • 组织特异性：
    Isoform 1 is widely expressed. Isoform 2 is highly enriched in testis, mainly restricted to meiotic and postmeiotic germ cells.
  • 数据库链接：

    KEGG: mmu:19047

    STRING: 10090.ENSMUSP00000099587

    UniGene: Mm.280784