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  4. Recombinant Mouse Serpin H1 (Serpinh1)

Recombinant Mouse Serpin H1 (Serpinh1)

  • 货号：
    CSB-BP021087MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-EP021087MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-MP021087MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:Serpinh1 Synonyms:Cbp1, Hsp47
  • Uniprot No.：
  • 别名：
    Serpinh1; Cbp1; Hsp47; Serpin H1; 47 kDa heat shock protein; Collagen-binding protein; Colligin; Serine protease inhibitor J6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-417
  • 氨基酸序列
    AEV KKPLEAAAPG TAEKLSSKAT TLAERSTGLA FSLYQAMAKD QAVENILLSP LVVASSLGLV SLGGKATTAS QAKAVLSAEK LRDEEVHTGL GELLRSLSNS TARNVTWKLG SRLYGPSSVS FADDFVRSSK QHYNCEHSKI NFRDKRSALQ SINEWASQTT DGKLPEVTKD VERTDGALLV NAMFFKPHWD EKFHHKMVDN RGFMVTRSYT VGVTMMHRTG LYNYYDDEKE KLQMVEMPLA HKLSSLIILM PHHVEPLERL EKLLTKEQLK AWMGKMQKKA VAISLPKGVV EVTHDLQKHL AGLGLTEAID KNKADLSRMS GKKDLYLASV FHATAFEWDT EGNPFDQDIY GREELRSPKL FYADHPFIFL VRDNQSGSLL FIGRLVRPKG DKMRDEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 基因功能参考文献：
    1. The HSP47 might exert influence on liver fibrosis via the regulation ofendothelin receptor A (ETAR) and endothelin receptor B (ETBR). PMID: 28802097
    2. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. PMID: 27477081
    3. HSP47 appears after a period of certain activities to repair damaged collagen fibers, and the activity was returned to a state of equilibrium at day 30 with significantly diminished expression. Thus, the results suggest that HSP47 is actively involved in homeostasis of periodontal tissue subjected to occlusal overload PMID: 27076780
    4. Intradermal administration of siHSP47-nanoparticles effectively reduced HSP47 protein expression in skin to normal level. PMID: 26196532
    5. 2014). Data show that the expression of heat shock protein 47 (HSP47) was increased in the scleroderma mouse model. PMID: 26091621
    6. Deletion of Hsp47 caused activated hepatic stellate cells to undergo ER stress-mediated apoptosis when autophagy was inhibited PMID: 25525267
    7. Mouse embryonic stem cell migration and differentiation into smooth muscle cells is induced by the over-expression of HSP47. PMID: 24454956
    8. s conclude that heat shock protein 47 plays an essential role in Schistosoma japonicum-induced hepatic fibrosis in mice. PMID: 24295791
    9. miR-29b down-regulates HSP47 and LOX expression. PMID: 24650661
    10. expression patterns of HSP70 and HSP47 in tissues following short-pulse laser irradiation PMID: 23587755
    11. The pH sensitivity of murine heat shock protein 47 (HSP47) binding to collagen is affected by mutations in the breach histidine cluster. PMID: 23212911
    12. These results demonstrate that Hsp47 is indispensable for well-organized cartilage and normal endochondral bone formation. PMID: 22492985
    13. overexpression of HSP47 in keloid fibroblast cells could induce excessive collagen accumulation by enhancing collagen synthesis, which not only presents a possible mechanism of keloid formation PMID: 22295344
    14. alpha-smooth-muscle-actin-positive myofibroblast of bleomycin-induced pulmonary fibrosis cells may synthesize procollagen in the fibrotic process of bleomycin-treated lungs through upregulation of HSP47 mRNA and play an important role in fibrogenesis. PMID: 20094730
    15. HSP47 localized in alpha-smooth muscle actin-positive myofibroblasts, F4/80 negative, surfactant protein-A-positive type II pneumocytes, and F4/80-positive macrophages. Cells may play role in fibrotic process through upregulation of HSP47. PMID: 12842808
    16. Results indicate that Hsp47 is required for the molecular maturation of type IV collagen and suggest that misfolded type IV collagen causes abnormal morphology of embryoid bodies. PMID: 15282337
    17. type IV collagen in dilated endoplasmic reticulum leads to apoptosis in Hsp47-knockout mouse embryos via induction of CHOP PMID: 15522896
    18. analysis of the client recognition mechanism of HSP47 PMID: 16326708
    19. analysis of recognition of the collagen triple helix by chaperone HSP47 PMID: 16484215
    20. Hsp47 is required for correct folding and prevention of aggregation of type I collagen in the ER. PMID: 16525016
    21. the HSP47-specific siRNA inhibited expression of HSP47 at the level of mRNA and protein, and furthermore, adenovirus-mediated transfer of siRNA against HSP47 inhibited the expression of type I collagen and formation of scar tissue in animal mod PMID: 18093850

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  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Serpin family
  • 数据库链接：

    KEGG: mmu:12406

    STRING: 10090.ENSMUSP00000091706

    UniGene: Mm.22708