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  4. Recombinant Mouse Shadow of prion protein (Sprn)

Recombinant Mouse Shadow of prion protein (Sprn)

  • 中文名称：
    小鼠Sprn重组蛋白
  • 货号：
    CSB-YP805354MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sprn重组蛋白
  • 货号：
    CSB-EP805354MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sprn重组蛋白
  • 货号：
    CSB-EP805354MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sprn重组蛋白
  • 货号：
    CSB-BP805354MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sprn重组蛋白
  • 货号：
    CSB-MP805354MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sprn
  • Uniprot No.：
  • 别名：
    Sprn; Gm169; Shadow of prion protein; Protein shadoo
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-122
  • 氨基酸序列
    KGGRGG ARGSARGVRG GARGASRVRV RPAPRYGSSL RVAAAGAAAG AAAGVAAGLA TGSGWRRTSG PGELGLEDDE NGAMGGNGTD RGVYSYWAWT SG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Prion-like protein that has PrP(C)-like neuroprotective activity. May act as a modulator for the biological actions of normal and abnormal PrP.
  • 基因功能参考文献：
    1. Data suggest that expression of shadoo in neuron or hepatocyte cell lines induces same toxic phenotype of drug hypersensitivity as prion protein/PrP(-central region); this effect is counteracted by co-expression of PrP-wild-type. PMID: 26721882
    2. No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice PMID: 25495671
    3. These observations suggest that Shadoo can affect the prion replication process by (i) acting as a holdase and (ii) interfering with the dominant-negative inhibitor effect of the C1 fragment. PMID: 25855735
    4. Data suggest that glycosylation status of the prion protein and yet-to-be-dentified proteases modulate internal C1 and C2 endoproteolysis of Shadoo (shadow of prion protein) and Doppel (doppelganger prion) in mouse neurons. PMID: 24286250
    5. binding of Sho to anionic liposomes has disruptive effect on integrity of lipid bilayer leading to formation of supramolecular lipid-protein complexes. prion diseases might depend on the oligomerization state of Sho and on these lipoprotein assembles. PMID: 23911790
    6. Sho is not pi, or, given the accumulating data for many activities for PrP (C) , the pi hypothesis invoking a discrete signaling pathway to maintain neuronal viability is no longer tenable. PMID: 22929230
    7. Here we report that Shadoo (Sho)-a member of the prion protein superfamily-is also found in the nucleus of several neural and non-neural cell lines as visualized by using an YFP-Sho construct PMID: 23360978
    8. the Sho region N-terminal to the hydrophobic domain includes tandem positively charged "RGG boxes", predicted to bind RNA. Here, we demonstrate that Sho binds DNA and RNA in vitro via this arginine-rich region. PMID: 23121093
    9. using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages PMID: 22860039
    10. Knockout of the prion protein (PrP)-like Sprn gene does not produce embryonic lethality in combination with PrP(C)-deficiency. PMID: 22619325
    11. the developmental expression of Shadoo between 10.5 and 14.5 dpc was described. PMID: 22093825
    12. The results of this study concluded that Shadoo has little or no influence on the outcome of transmissible spongiform encephalopathy (TSE) disease in transgenic mice. PMID: 21458534
    13. Sho may play a role in the physiological function of PrP(C) and prion pathogenesis. PMID: 19685161
    14. Sho is a candidate for pi, and since it engenders a PrP(C)-like neuroprotective activity, compromised neuroprotective activity resulting from reduced levels exacerbate damage in prion infections. PMID: 17703189
    15. Sprn does not play a major role in prion disease incubation time in mice. PMID: 19513788
    16. Shadoo-encoding gene knockdown in PrP-knockout mouse embryos results in a lethal phenotype, occurring between E8 and E11, not observed on the wild-type genetic background. PMID: 19766638

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    SPRN family
  • 组织特异性：
    Mainly expressed in brain (at protein level). In brain, it is highly expressed in the hippocampus and cerebellum and is also expressed at lower level in other areas of the brain including the cerebral cortex, the thalamus and the medulla. In hippocampus a
  • 数据库链接：

    KEGG: mmu:212518

    STRING: 10090.ENSMUSP00000053901

    UniGene: Mm.246858