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  4. Recombinant Mouse Single-minded homolog 2 (Sim2)

Recombinant Mouse Single-minded homolog 2 (Sim2)

  • 货号：
    CSB-YP717195MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP717195MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP717195MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP717195MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP717195MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sim2
  • Uniprot No.：
  • 别名：
    Sim2; Single-minded homolog 2; SIM transcription factor; mSIM
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-657
  • 氨基酸序列
    MKEKSKNAAK TRREKENGEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRAVFPEGL GDAWGQPSRT GPLDSVAKEL GSHLLQTLDG FVFVVASDGK IMYISETASV HLGLSQVELT GNSIYEYIHP SDHDEMTAVL TAHPPLHHHL LQEYEIERSF FLRMKCVLAK RNAGLTCSGY KVIHCSGYLK IRQYMLDMSL YDSCYQIVGL VAVGQSLPPS AITEIKLHSN MFMFRASLDL KLIFLDSRVT ELTGYEPQDL IEKTLYHHVH GCDTFHLRYA HHLLLVKGQV TTKYYRLLSK LGGWVWVQSY ATVVHNSRSS RPHCIVSVNY VLTDVEYKEL QLSLDQVSTS KSQESWRTTL STSQETRKSA KPKNTKMKTK LRTNPYPPQQ YSSFQMDKLE CSQVGNWRTS PPTNAVAPPE QQLHSEASDL LYGPPYSLPF SYHYGHFPLD SHVFSSKKPG LPAKFGQPQG SPCEVARFFL STLPASSECQ WHCANSLVPS SSSPAKNLSE PSPVNAARHG LVPNYEAPSA AARRFCEDPA PPSFPSCGHY REEPALGPAK APRQASRDAA RLALARAPPE CCAPPAPEPQ APAQLPFVLL NYHRVLARRG PLGSAAPGAP EAAGSLRPRH PGPVAASAPG APRPHYLGAS VIITNGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
  • 基因功能参考文献：
    1. Ethanol induced Sim2 expression in cerebral cortices of P7 Mice via the PKA pathway. PMID: 25319570
    2. Study provides evidence that SIM2 binds a specific set of enhancer elements thus explaining how SIM2 can regulate its gene network in neuronal features. PMID: 25955728
    3. Our results establish that SIM2 is a crucial regulator in controlling expression of intestinal AMPs PMID: 25277798
    4. Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis. PMID: 22513369
    5. mammary gland involution is disrupted in Sim2s overexpression and STAT3-mediated signaling and other pathways involved in the acute phase response are inhibited PMID: 21292822
    6. Sim2s is required for establishing and maintaining mammary gland differentiation. PMID: 20150276
    7. Sim2 mutants also develop congenital scoliosis, reflected by the unequal sizes of the left and right vertebrae and ribs. The spatial expression patterns of Sim2 in these skeletal elements suggest that Sim2 regulates their growth and/or integrity. PMID: 12024028
    8. Data show that premature accumulation of hyaluronan in Sim2 -/- animal palates suggests a regulatory role for Sim2 in hyaluronan synthesis and in the establishment of craniofacial architecture. PMID: 12203729
    9. Sim2 and Sim2s are isoforms that may play different roles during development or in tissue-specific effects on other protein-mediated pathways PMID: 16484282
    10. It is concluded that Sim2 may play an important role in the pathogenesis of Down syndrome (DS) by inhibiting the cell cycle, which is related to the decreased expression of cyclin E and increased expression of p27. PMID: 16530433
    11. Sim2 play an role in the pathogenesis of Down syndrome by inhibiting neurite growth and cell differentiation. PMID: 16825162
    12. Sim2 could impair the ability of learning and memory by inhibiting synaptic plasticity, and may play a crucial role in the pathogenesis of Down Syndrome. PMID: 16963290
    13. SIM2s is the first protein shown to bind and repress the SLUG promoter. SIM2s is a key regulator of mammary-ductal development. Loss of SIM2s is associated with an invasive, epithelial-msesnchymal-transition-like phenotype. PMID: 18160708
    14. chick and mouse Sim2 gene expression is enhanced in limb ventral muscle masses versus dorsal ventral muscle masses PMID: 19123137
    15. Ha-Ras transformation of MCF10A cells leads to repression of Singleminded-2s through NOTCH and C/EBPbeta. PMID: 19169276

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Transcripts were detected in high levels in kidney followed by skeletal muscle and lung. Low levels were found in testis, brain and heart. In early fetal development it is found in CNS, developing kidney, tongue epithelium and cartilage primordia.
  • 数据库链接：

    KEGG: mmu:20465

    STRING: 10090.ENSMUSP00000072043

    UniGene: Mm.4775