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  4. Recombinant Mouse Sphingosine-1-phosphate lyase 1 (Sgpl1), partial

Recombinant Mouse Sphingosine-1-phosphate lyase 1 (Sgpl1), partial

  • 货号：
    CSB-YP021197MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021197MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021197MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021197MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021197MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sgpl1; Spl; Sphingosine-1-phosphate lyase 1; S1PL; SP-lyase 1; SPL 1; mSPL; EC 4.1.2.27; Sphingosine-1-phosphate aldolase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP. Seems to play a role in establishing neuronal contact sites and axonal maintenance.
  • 基因功能参考文献：
    1. These results show that both a primary defect in cholesterol trafficking and S1P lyase deficiency cause overlapping phenotypic alterations, and challenge the present view on the role of sphingosine in lysosomal Ca(2+) homeostasis. PMID: 28262793
    2. Partial genetic deletion of Sgpl1 protected mice against ventilator induced lung injury. PMID: 29301259
    3. Study identifies sphingosine 1-phosphate metabolism as a novel player in modulating synaptic architecture and plasticity. PMID: 27883090
    4. SGPL1 modulates neuronal autophagy via phosphatidylethanolamine production PMID: 28521611
    5. Study verifies the role of a high-ranked gene in dysregulation of sphingolipid metabolism in the disease and demonstrate that inhibiting the enzyme, sphingosine-1-phosphate lyase 1 (SPL), has neuroprotective effects in Huntington's disease models. PMID: 28931805
    6. Epigenetic regulation of pro-inflammatory cytokine secretion by sphingosine 1-phosphate (S1P) in acute lung injury, and the role in this process of S1P lyase has been described. (Review) PMID: 27720306
    7. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency PMID: 28165339
    8. Sphingosine-1-phosphate lyase mutations cause primary adrenal insufficiency and steroid-resistant nephrotic syndrome. PMID: 28165343
    9. deleting SPL in CD11c(+) dendritic cells (DCs), rather than thymic epithelial cells or other stromal cells, disrupts the sphingosine-1-phosphate gradient, preventing egress. PMID: 27810923
    10. The sphingosine-1-phosphate lyase contributes to the restriction of Legionella pneumophila in murine macrophages. PMID: 26741365
    11. Studies suggest that S1PL is a novel endogenous suppressor of pulmonary fibrosis in human idiopathic pulmonary fibrosis and animal models. PMID: 26286721
    12. Although the observed inhibitory effect was not as strong as that of 4-deoxypyridoxine (a VB6 analog SPL inhibitor), these findings may be useful for further understanding the mechanism of action of THI. PMID: 25381637
    13. upregulation of multidrug resistance transporters contributes to the chemoresistance of S1P lyase-deficient MEFs PMID: 25385827
    14. Sphingosine-1-phosphate lyase downregulation promotes colon carcinogenesis through STAT3-activated microRNAs. PMID: 25347472
    15. Findings suggest that sphingosine phosphate lyase (S1P lyase) expressed in the thymic medullary perivascular spaces keeps the tissue sphingosine 1-phosphate low around the vessels and promotes thymic egress via up-regulation of S1P receptor 1 (S1P1). PMID: 24343820
    16. Data indicate that SGPL1 sphingosine 1-phosphate lyase (aldolase) mRNA is significantly increased in the periinfarct cortex. PMID: 23936150
    17. Leukocyte Sgpl1 as critical factor in monocyte macrophage differentiation and function. PMID: 23700419
    18. Inducible Sgpl1 knockout mice are protected in experimental autoimmune encephalomyelitis (EAE). PMID: 23544080
    19. Sphingosine-1-phosphate lyase has many undiscovered physiological functions apart from its role in immunity PMID: 22781001
    20. S1PL deficiency increases sphingolipid formation via recycling at the expense of de novo biosynthesis in neurons. PMID: 22291021
    21. Data show that S1P lyase knockout mice with reduced S1P lyase activity (resulting in increased bio-available S1P) had improved survival compared with wild-type littermates. PMID: 21556483
    22. S1P lyase regulates DNA damage responses through a sphingolipid feedback mechanism. PMID: 21368890
    23. Sphingosine-1-phosphate lyase (SPL) contributes to oxidative stress by depleting sphingosine-1-phosphate pools available for cardioprotective signaling. SPL inhibition as a strategy for reducing cardiac ischemia-reperfusion (I/R) injury. PMID: 21335477
    24. a genetic block in S1P degradation elicits a pro-inflammatory response but impairs neutrophil migration from blood into tissues PMID: 21173151
    25. Sphingosine 1-phosphate lyase deficiency disrupts lipid homeostasis in liver. PMID: 20097939
    26. S1P lyase-deficient cells show that S1P metabolism contributes to long-term regulation of cellular Ca2+ storage. PMID: 19913094
    27. SPL may be involved in the regulation of intracellular S1P levels during this differentiation PMID: 12584204
    28. Data report the characterization of the expression, localization, and topology of sphingosine-1-phosphate lyase. PMID: 15522238
    29. These results provide evidence of the existence of an alternative S1P lyase. PMID: 15737611
    30. Products of the Spl pathway stimulate mitogenesis. PMID: 15938718
    31. it was concluded that lymphocyte egress is mediated by sphingosine 1-phosphate (S1P) gradients that are established by S1P lyase activity and that the lyase may represent a novel immunosuppressant drug target PMID: 16151014
    32. genetic or epigenetic changes affecting intestinal sphingosine-1-phosphate metabolism may correlate with and potentially contribute to carcinogenesis PMID: 17090686
    33. extracellular S1P is dephosphorylated and subsequently converted by cells, which appears to be important for clearance of the signaling molecule S1P in the local tissue environment after infections or injuries PMID: 18172856
    34. Data suggest that lymphocyte trafficking is particularly sensitive to variations in sphingosine 1-phosphate lyase (S1PL) activity and suggest that there is a window in which partial inhibition of S1PL could produce therapeutic levels of immunosuppression. PMID: 19119317
    35. sphingosine 1-phosphate subcellular origin is essential for its toxic effect in lyase-deficient neurons PMID: 19251691
    36. SGPL1-deficient mice exhibit postnatal discontinuation of early thymocytopoiesis starting at age 2 weeks and leading to accumulation of sphingosine 1-phosphate in lymphoid tissues, which blocks lymphocyte egress and induces thymus atrophy. PMID: 19748984

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily
  • 组织特异性：
    Highest levels are found in liver, small intestine and thymus, followed by kidney, lung, heart, spleen and brain (at protein level). Also detected in stomach, testis and skeletal muscle (at protein level).
  • 数据库链接：

    KEGG: mmu:20397

    STRING: 10090.ENSMUSP00000090155

    UniGene: Mm.412319