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  4. Recombinant Mouse TNF receptor-associated factor 3 (Traf3)

Recombinant Mouse TNF receptor-associated factor 3 (Traf3)

  • 货号：
    CSB-YP024148MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024148MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024148MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024148MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024148MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Traf3; Craf1; Trafamn; TNF receptor-associated factor 3; EC 2.3.2.27; CD40 receptor-associated factor 1; CRAF1; RING-type E3 ubiquitin transferase TRAF3; TRAFAMN
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-567
  • 氨基酸序列
    MESSKKMDAA GTLQPNPPLK LQPDRGAGSV LVPEQGGYKE KFVKTVEDKY KCEKCRLVLC NPKQTECGHR FCESCMAALL SSSSPKCTAC QESIIKDKVF KDNCCKREIL ALQVYCRNEG RGCAEQLTLG HLLVHLKNEC QFEELPCLRA DCKEKVLRKD LRDHVEKACK YREATCSHCK SQVPMIKLQK HEDTDCPCVV VSCPHKCSVQ TLLRSELSAH LSECVNAPST CSFKRYGCVF QGTNQQIKAH EASSAVQHVN LLKEWSNSLE KKVSLLQNES VEKNKSIQSL HNQICSFEIE IERQKEMLRN NESKILHLQR VIDSQAEKLK ELDKEIRPFR QNWEEADSMK SSVESLQNRV TELESVDKSA GQAARNTGLL ESQLSRHDQM LSVHDIRLAD MDLRFQVLET ASYNGVLIWK IRDYKRRKQE AVMGKTLSLY SQPFYTGYFG YKMCARVYLN GDGMGKGTHL SLFFVIMRGE YDALLPWPFK QKVTLMLMDQ GSSRRHLGDA FKPDPNSSSF KKPTGEMNIA SGCPVFVAQT VLENGTYIKD DTIFIKVIVD TSDLPDP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
  • 基因功能参考文献：
    1. A TRAF3-NIK axis differentially regulates viral DNA vs RNA pathways in innate immune signaling. PMID: 30018345
    2. TRAF3 promotes T cell activation by regulating localization and functions of Csk and PTPN22. PMID: 28522807
    3. osteoclast-targeted antagomir-214-3p delivery could recover the TRAF3 protein expression and attenuate the development of osteolytic bone metastasis. PMID: 28071724
    4. These results indicate that TRAF3 deficiency suffices to metabolically reprogram B cells, a finding that improves our understanding of the role of TRAF3 as a tumor suppressor, and suggests potential therapeutic strategies. PMID: 27752131
    5. Depletion of LAP1 during early embryonic myogenesis leads to growth retardation and premature death. PMID: 27798115
    6. In B lymphocytes, TNFR-associated factor 3 inhibits signaling by TNFR superfamily receptors, Toll-like receptors, and interleukin-6R. In T lymphocytes, TNFR-associated factor 3 is required for normal signaling by the T cell antigen receptor, while inhibiting signaling by the interleukin-2 receptor. Cytoplasmic TNFR -associated factor 3 restrains nuclear factor-kappaB2 activation in both T and B cells. Review. PMID: 27154354
    7. TRAF3 may likely induce apoptosis and resistance to proliferation and may be a new target to inhibit the cyst formation in polycystic kidney disease by regulating the NF-kappaB signaling pathway Bcl-2 and Bcl-xl activity. PMID: 28185403
    8. this study shows that TRAF3 ubiquitination triggers expulsion of intracellular bacteria by exocyst complex PMID: 27438768
    9. NDR1 interacts with TRAF3 and interferes with the association of TRAF3 and IL-17R, resulting in increased formation of the activation complex IL-17R-Act1, which is required for the downstream signaling and production of pro-inflammatory factors PMID: 28219902
    10. Upon stimulation with poly(I:C), malaria parasite-infected red blood cells (iRBCs), or vesicular stomatitis virus (VSV), FOSL1 "translocated" from the nucleus to the cytoplasm, where it inhibited the interactions between TNF receptor-associated factor 3 (TRAF3), TIR domain-containing adapter inducing IFN-beta (TRIF), and Tank-binding kinase 1 (TBK1) via impairing K63-linked polyubiquitination of TRAF3 and TRIF. PMID: 28049150
    11. These findings reveal a novel mechanism that endotoxin tolerance reprograms mitogen-activated protein kinase signaling by suppressing Pellino 1-mediated K63-linked ubiquitination of cIAP2, K48-linked ubiquitination, and degradation of TRAF3. PMID: 27377744
    12. Findings establish CK1varepsilon as a regulator of antiviral innate immune responses and indicate a novel mechanism of immunoregulation that involves CK1varepsilon-mediated phosphorylation of TRAF3. PMID: 26928339
    13. findings identify TRAF3 and PTPN22 as inhibitors of IL-6R signaling in B cells and reveal a previously uncharacterized role for TRAF3 in the regulation of plasma cell differentiation PMID: 26329582
    14. TRAF3 expressed in myeloid cells regulates immune responses in myeloid cells and acts to inhibit inflammation and tumor development in mice. PMID: 25422508
    15. The MYSM1 SWIRM domain interacts with TRAF3 to terminate PRR pathways for pro-inflammatory and type I interferon responses. PMID: 26474655
    16. Data show that the degradation of TNF receptor-associated factor 3 (TRAF3) and TNF receptor-associated Ffactor 6 (TRAF6) was accelerated in ubiquitin-specific protease 25 knockout (USP25-/-) cells after viral infection. PMID: 26305951
    17. Maternal diabetes and high glucose negatively regulate miR-322 through oxidative stress. miR-322 interacts with the 3'-UTR of TRAF3 and represses its translation. PMID: 25516495
    18. These results indicate that the involvement of TRAF3 in IL-15 mediated signaling to T cells plays a previously unappreciated and critical role in CD8+ central memory T cells regulation and maintenance. PMID: 25010048
    19. TRAF3 is a central regulator of ischemic pathways, including nuclear factor kappaB, Rac-1, and c-Jun kinase signaling, via its interaction with and activation of TAK1. PMID: 26269654
    20. Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a positive regulator of pathological cardiac hypertrophy in mice and humans. PMID: 26034202
    21. In obesity progression, myeloid TRAF3 functionally switches its activity from anti-inflammatory to proinflammatory modes, thereby coupling overnutrition to metabolic inflammation, insulin resistance, and metabolic disease. PMID: 25628422
    22. Data show that docking protein 3 (DOK3) plays a role in TLR3 signaling by enabling TNFR-associated factor (TRAF) 3/TANK-binding kinase (TBK) 1 binding and facilitating TBK1 and IFN regulatory factor 3 activation and the induction of IFN-beta production. PMID: 24929003
    23. TRAF3 is a mediator of Treg cell function in the regulation of antibody responses and plays a role in mediating ICOS expression in Treg cells. PMID: 24378539
    24. Autophagic/lysosomal degradation of TRAF3 is an important step in RANKL-induced NF-kappaB activation in osteoclast precursors. PMID: 24316970
    25. TRAF3 plays a central role in regulation of medullary thymic epithelial cells by imposing requirements for T cell cross-talk. PMID: 24324158
    26. Data from knockout mice suggest that Traf3 and Tlr2 (toll-like receptor 2) are involved in innate immunity; via selective up-regulation of Traf3, macrophages can be primed with TLR2 ligands to increase virus-mediated interferon induction. PMID: 23853595
    27. Defective T-bet up-regulation occurring during the stage 1 to stage 2 transition results from reduced TCR signaling in TRAF3(-/-) iNKT cells. PMID: 23650438
    28. Peli1 is a microglia-specific mediator of autoimmune neuroinflammation that works by regulating Traf3 degradation PMID: 23603814
    29. In vitro and cellular experiments showed that forms of TRAF5 with mutation of the corresponding residues to those of TRAF3 had TRAF3-like antiviral activity. PMID: 23150880
    30. Lymphotoxin-beta receptor signalling regulates cytokine expression via TRIM30alpha in a TRAF3-dependent manner. PMID: 23220069
    31. establish OTUD7B as a crucial regulator of signal-induced non-canonical NF-kappaB activation and indicate a mechanism of immune regulation that involves OTUD7B-mediated deubiquitination and stabilization of TRAF3 PMID: 23334419
    32. using B lymphoma cells derived from B-TRAF3 -/- mice as model systems, we demonstarted that oridonin and NF-kB2 shRNAs have therapeutic potential PMID: 22033491
    33. S-glutathionylation of a conserved cysteine residue of TRAF3 and TRAF6 is important for ROS-dependent activation of innate immune pathways in herpes simplex virus infection PMID: 21949653
    34. Given the varied roles played by TRAF3 in immune cells, an important knowledge gap includes the distinct functions of TRAF3 in Toll-like receptor signaling to B cells. PMID: 21971520
    35. TRAF3 markedly inhibited IL-17-induced expression of inflammatory cytokine and chemokine genes in vivo and consequently delayed the onset and greatly reduced the incidence and severity of experimental autoimmune encephalomyelitis (EAE). PMID: 21078888
    36. the LTbetaR modifies the ubiquitin:NIK E3 ligase, and also acts as an allosteric regulator of the ubiquitin:TRAF E3 ligase PMID: 20348096
    37. Data show that TRIF-dependent signaling triggered noncanonical TRAF3 self-ubiquitination that activated the interferon response. PMID: 19898473
    38. LMP1 signaling was markedly defective in TRAF3-/- B cells. PMID: 14981114
    39. TRAF3 mediates carboxyl-terminal activating region 1 (CTAR1) of Epstein Barr virus-encoded oncoprotein latent membrane protein 1 signaling by directly interacting with CTAR1 through the first PQQAT amino acid motif. PMID: 15494504
    40. cells lacking TRAF3, a member of the TNF receptor-associated factor family, are defective in type I IFN responses activated by several different TLRs PMID: 16306936
    41. TRAF3 is essential for the induction of type I interferons (IFN) and the anti-inflammatory cytokine interleukin-10 (IL-10), but is dispensable for expression of pro-inflammatory cytokines PMID: 16306937
    42. TRAF3 has a role in innate antiviral immunity [review] PMID: 16582590
    43. TRAF3 play a critical role in negative regulation of noncanonical NF-kappa B activity. PMID: 17015635
    44. Both the RING finger domain activity and the specific binding of the TRAF domain to NF-kappa B-inducing kinase (NIK) protein are two critical components of TRAF3 suppression of NIK protein levels and the processing of p100 to p52. PMID: 17158868
    45. TRAF3 is a critical regulator of peripheral B cell homeostasis and may be implicated in the regulation of peripheral self-tolerance induction. PMID: 17723217
    46. TRAF3 potently suppresses canonical (p50-dependent) NF-kappaB activation and gene expression in vitro and in vivo PMID: 18292232
    47. Lymphocyte-specific TRAF3 transgenic mice have enhanced humoral responses and develop plasmacytosis, autoimmunity, inflammation, and cancer. PMID: 19074733
    48. c-Src enhances RIG-I-mediated signaling, acting at the level of TRAF3 PMID: 19419966

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  • 亚细胞定位：
    Cytoplasm. Endosome.
  • 蛋白家族：
    TNF receptor-associated factor family, A subfamily
  • 组织特异性：
    Detected in bone marrow macrophages and spleen B-cells (at protein level). In adult, highest in brain. Also found in kidney, heart, thymus, spleen, lung, muscle, testis and ovary. Not found in liver.
  • 数据库链接：

    KEGG: mmu:22031

    STRING: 10090.ENSMUSP00000021706

    UniGene: Mm.27431