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  4. Recombinant Mouse Targeting protein for Xklp2 (Tpx2)

Recombinant Mouse Targeting protein for Xklp2 (Tpx2)

  • 中文名称：
    小鼠Tpx2重组蛋白
  • 货号：
    CSB-YP024139MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tpx2重组蛋白
  • 货号：
    CSB-EP024139MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tpx2重组蛋白
  • 货号：
    CSB-EP024139MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tpx2重组蛋白
  • 货号：
    CSB-BP024139MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tpx2重组蛋白
  • 货号：
    CSB-MP024139MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tpx2
  • Uniprot No.：
  • 别名：
    Tpx2; Targeting protein for Xklp2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-745
  • 氨基酸序列
    MSQVPTTYSF DAPTDFINFS SLDAEEDTEN IDSWFDEKAN LENKFLRQRG IGEPFQGKNS LRKAKLQQGF VTPLKAVDNT YHKETEKENL QKQSIPSNDC SSLDAKRAVS GNTPVQPQRR SIRLSAQKDL EQKEKNHVAS VEMKAKRCVA PATDCPPQKR MKVSHKKKLE EEEEGSAPAT SRKNERETLE KAKGKHTVPG VPPAREKVLK STEEQEIEKR LRMQQEVVEL RRKNEEFKKL ALAGPGQPVK KSTSQVTKTV DFHFLTDERI KQHPKNQEEY KEVNFMSELR KHSSTPARGT RGCTIIKPFN LSKGKKRTFD EAASTYVPIA QQVEAFHKRT PNRYHLRNKK DESLLPSKSV NKIARDPQTP ILQTKYRTRA VTCKSTAEQE AEELEKLQQY KFKARELDPR IFESGPILPK RAPVKPPTQP VGFDLEIEKR IHERESKKKT EDEQFEFHSR PCPTKILEDV VGVPEKKVIP ATVPKSPVFA LKNRIRVPIK DEEEEKPVVI KAQPVPHYGV PYKPHIAEAR NVEVCPFSFD TRDKERQLQK EKKIKEMQKG EVPKFKALPV PHFDTINLPE KKVKNVTQAE PFSLETDKRG AYKAEMWKHQ LEEEQKQQKD AACFKARPNT VIFQEPFVPK KEKKSLAENP SGSLVQEPFQ LATERRAKER QELEKKMAEV EAWKLQQLEE VRQQEEEQQK EELARLRKEL VHKANPIRKY AAVEVKSSEL PLTVPVSPKF STRFQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
  • 基因功能参考文献：
    1. Depleting TPX2 decreases microtubule formation frequency specifically at the tip and the base of the neurite, and these correlate precisely with the regions where active GTP-bound Ran proteins are enriched. PMID: 28205572
    2. Bcl2l10, Tpx2, and Aurka co-localized on the meiotic spindles, and Bcl2l10 was present in the same complex with Tpx2. PMID: 27753540
    3. Study demonstrates that the Golgi resident protein GM130 activates the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and further captures them to couple mitotic membranes to the spindle. PMID: 26165940
    4. The findings indicated that Tpx2 is essential for maintaining genomic stability through its role in spindle regulation. PMID: 22266221
    5. Tpx2 redistributes from the nucleus to the apical process, and promotes nuclear migration during G2-phase by altering microtubule organization. PMID: 21441895

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    TPX2 family
  • 数据库链接：

    KEGG: mmu:72119

    STRING: 10090.ENSMUSP00000028969

    UniGene: Mm.407737