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  4. Recombinant Mouse Tartrate-resistant acid phosphatase type 5 (Acp5)

Recombinant Mouse Tartrate-resistant acid phosphatase type 5 (Acp5)

  • 货号：
    CSB-YP001178MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001178MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001178MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001178MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001178MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Acp5; T5ap; Trap; Tartrate-resistant acid phosphatase type 5; TR-AP; EC 3.1.3.2; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    23-327
  • 氨基酸序列
    APTPTLRF VAVGDWGGVP NAPFHTAREM ANAKEIARTV QTMGADFIMS LGDNFYFTGV HDASDKRFQE TFEDVFSDRA LRNIPWYVLA GNHDHLGNVS AQIAYSKISK RWNFPSPYYR LRFKIPRTNI TVAIFMLDTV MLCGNSDDFA SQQPKMPRDL GVARTQLSWL KKQLAAAKED YVLVAGHYPI WSIAEHGPTR CLVKNLRPLL ATYGVTAYLC GHDHNLQYLQ DENGVGYVLS GAGNFMDPSV RHQRKVPNGY LRFHYGSEDS LGGFTHVEIS PKEMTIIYVE ASGKSLFKTS LPRRPRP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
  • 基因功能参考文献：
    1. TRAP and nidogen-2 co-localized in adipose tissue cells in situ. PMID: 25450682
    2. Uteroferrin is a cytokine secreted by uterine glands in response to progesterone that promotes fetal erythropoiesis at various stages of pregnancy. PMID: 25093463
    3. Supraphysiologic oscillatory fluid shear induces upregulation of osteoclastic activity, measured by tartrate-resistant acid phosphatase activity and formation of mineral resorption pits. PMID: 24275439
    4. Pax6 binds endogenously to the proximal region of the tartrate acid phosphatase (TRAP) gene promoter and suppresses nuclear factor of activated T cells c1 (NFATc1)-induced TRAP gene expression. PMID: 23990468
    5. Serum TRAP levels were lower in mice receiving eggshell calcium citrate malate than those receiving eggshells calcium malate. PMID: 23603074
    6. tartrate resistant acid phosphatase PMID: 23444125
    7. TRAP is not essential for the formation of the epiphyseal vascular network. PMID: 22579636
    8. dephosphorylation of Man6P-containing lysosomal proteins requires the concerted action of Acp2 and Acp5 and is needed for hydrolysis and removal of degradation products PMID: 22158965
    9. Accumulation of vesicles in the cytoplasm of mutant osteoclasts indicates a novel function for tartrate-resistant acid phosphatase in modulating intracellular vesicular transport in osteoclasts PMID: 12243748
    10. USF1 and USF2 play a functional role in RANKL-dependent TRAP expression during osteoclast differentiation. PMID: 12663664
    11. TRACP expression is a target for regulation by the macrophage/osteoclast transcription factor PU.1 and the osteoclast commitment factor MiTF PMID: 14584903
    12. YY1 plays a functional role in RANKL-mediated TRAP gene expression during osteoclast differentiation. PMID: 15563837
    13. distinct tartrate-resistant acid phosphatase promoter regions have distinct roles in gene regulation in transgenic mice PMID: 15590658
    14. MMP-2 and MMP-9 expression by TRAP positive mononuclear and multinucleated cells are involved in articular cartilage destruction in CIA. PMID: 15862156
    15. TRAP release is due to secretion, rather than cell death PMID: 16475168
    16. TRACP is important for polarizing responses in naive T cells to antigen-presented dendritic cells. PMID: 16939395
    17. both synthesis as well as degradation of collagen are increased when TRAP is absent in mice at 8 weeks and 6 months of age, showing that TRAP has an important role in the metabolism of collagen PMID: 17551769
    18. transcriptional activity of the Tracp gene, in pre-osteoclastic cells, is negatively regulated by the binding of PARP-1 protein to a potential consensus sequence located in its promoter region PMID: 18021007
    19. prolonged hypoxia induces the formation of TRAP-positive osteoclast-like cells, suggesting the occurrence of an autocrine mechanism for osteoclastogenesis PMID: 18056037
    20. Over-expression of monomeric tartrate resistant acid phosphatase, but not the dimeric form in adipose tissue leads to early onset spontaneous hyperplastic obesity PMID: 18320034
    21. central role for ACP5 in removal of the Man-6-P recognition marker from lysosomal proteins, which are then dephosphorylated PMID: 18940929
    22. in the absence of RANKL and osteoclast differentiation, STAT6 binds the TRAP promoter after IL-4 treatment and directly enhances TRAP expression PMID: 19801646

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  • 亚细胞定位：
    Lysosome.
  • 组织特异性：
    Characteristic constituent of osteoclasts.
  • 数据库链接：

    KEGG: mmu:11433

    STRING: 10090.ENSMUSP00000065425

    UniGene: Mm.46354