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  4. Recombinant Mouse Telomerase reverse transcriptase (Tert), partial

Recombinant Mouse Telomerase reverse transcriptase (Tert), partial

In Stock
  • 中文名称：
    小鼠Tert重组蛋白
  • 货号：
    CSB-EP023391MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tert; Telomerase reverse transcriptase; EC 2.7.7.49; Telomerase catalytic subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    54.1kDa
  • 表达区域：
    595-928aa
  • 氨基酸序列
    EVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSMGTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVKADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKHLQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFGDMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGAAPYQLPAHCLFPWCGLLL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
  • 基因功能参考文献：
    1. Par-4 activation and binding to TERT are key steps required for inducing the apoptosis of islet beta cells under high-glucose/fatty acid conditions in type 2 diabetes. PMID: 30186877
    2. Tert-deficient but not Terc-deficient mice develop hepatocyte injury and frank steatosis when challenged with liquid high-fat diet. PMID: 28741793
    3. identification of a subset of hepatocytes that expresses high levels of telomerase and show that this hepatocyte subset repopulates the liver during homeostasis and injury PMID: 29618815
    4. These preclinical data in mouse models and human cells provide a strong rationale for the development of pharmacological approaches to target BMI1-mediated mitochondrial regulation and protection from DNA damage to sustain the regenerative potential of the skeletal muscle in conditions of chronic muscle wasting. PMID: 28757168
    5. Reactivation of Tert in the hippocampus was sufficient to normalize the depressive but not the aggressive behaviors of Tert(-/-) mice. Conversely, re-expression of Tert in the medial prefrontal cortex (mPFC) reversed the aggressive but not the depressive behavior of Tert(-/-) mice. PMID: 27300262
    6. Nrf2-driven TERT regulates pentose phosphate pathway in glioblastoma. PMID: 27148686
    7. TERT has a role in neointima formation through epigenetic regulation of proliferative E2F1 target gene expression in smooth muscle cells. PMID: 27932351
    8. these findings support a model in which gain of TERT function modulates mTORC1 activity and induces autophagy. PMID: 27545609
    9. Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment PMID: 27633795
    10. Results suggest that in mature Purkinje neurons, TERT is present both in the nucleus and in mitochondria, where it may participate in adaptive responses of the neurons to excitotoxic and radiation stress PMID: 26374457
    11. Wnt10a/beta-catenin signaling pathway is able to exacerbate keloid cell proliferation and inhibit the apoptosis of keloid cells through its interaction with TERT. PMID: 27771714
    12. This study reports the characterisation of two novel mouse TERT splice variants, Ins-i1[1-102] (Insi1 for short) and Del-e12[1-40] (Dele12 for short) that have not been previously described. Insi1 represents an in-frame insertion of nucleotides 1-102 from intron 1, encoding a 34 amino acid insertion at amino acid 73. PMID: 26787169
    13. TERT may promote gastric cancer metastasis through the TERT-miR-29a-ITGB1 regulatory pathway. PMID: 26903137
    14. TERT switches macrophages towards M1 phenotype by regulating NF-kappaB signaling, but has limited effect on M2 macrophages polarization in vitro. PMID: 26725521
    15. The results suggest that the mouse endometrial epithelium and vasculature are foci of stem/progenitor activity expressing mTert. PMID: 26740067
    16. miR-195 overexpressed in old mesenchymal stem cells (OMSCs) induces stem cell senescence deteriorating their regenerative ability by directly deactivating telomerase reverse transcriptase (Tert), and abrogation of miR-195 can reverse stem cell aging. PMID: 26390028
    17. These findings indicate that telomerase gene therapy represents a novel therapeutic strategy to treat aplastic anemia provoked or associated with short telomeres. PMID: 26903545
    18. findings identified a key role for TERT in fibroblast proliferation and survival essential for pulmonary fibrosis PMID: 26555817
    19. miR-512-5p suppresses tumor growth by targeting TERT in telomerase positive head and neck squamous cell carcinoma in vitro and in vivo. PMID: 26258591
    20. Telomerase deficiency triggers alveolar stem cell replicative senescence-associated low-grade inflammation. PMID: 26518879
    21. high telomerase expression is a fundamental characteristic of germline stem cells. PMID: 26584619
    22. data suggest that S1P binding to hTERT allosterically mimicks phosphorylation, promoting telomerase stability and hence telomere maintenance, cell proliferation, and tumor growth. PMID: 26082434
    23. Telomerase may direct Pol I transcription in oncogenic and regenerative hyperproliferation. PMID: 25118183
    24. Tert expression confers cardioprotection in the adult mouse heart after acute myocardial infarction. PMID: 25519492
    25. TERT is a regulator of MYC stability in cancer. Reactivation of TERT, a direct transcriptional MYC target in tumors, provides a feed-forward mechanism to potentiate MYC-dependent oncogenesis. PMID: 25893605
    26. Pharmacologically relevant doses of atorvastatin resulted in a 6-fold increase of telomerase activity in mouse PBMCs and CD4 T cells. Transgenic GFP-mTert reporter mice had 30% to 15% less telomerase-positive lymphocytes during the first 5 months of age. PMID: 25127175
    27. Studies indicate that reverse transcriptase (RT) enzyme highly expressed in mouse embryos and mouse and human cancer cells and repressed in somatic differentiated healthy cells. PMID: 25586649
    28. The overexpression of Zfp637 markedly increases mTERT expression and telomerase activity, maintains telomere length, and inhibits H2O2 and D-galactose-induced senescence. PMID: 25032857
    29. Telomerase exerts telomere-independent effects on pulmonary artery smooth muscle cell growth in pulmonary hypertension. PMID: 25550449
    30. These findings establish a functional link between endoplasmic reticulum stress and telomerase. PMID: 24119029
    31. TERT, combined with beta-catenin and BRG1, serves as a transcriptional complex, which binds the FAS ligand (FASL) promoter to upregulate FASL expression, leading to an elevated immunomodulatory function. PMID: 24401839
    32. These data indicate that TERT plays an extratelomeric role in the reprogramming process, but its function is dispensable. PMID: 24733392
    33. Once HIV production had reached a peak (7 dpi), the telomerase activity decreased, showing levels similar to those of noninfected cells PMID: 24254728
    34. Overexpression of TERT in mesenchmal stem cells resulted in increased cell proliferation. PMID: 22884695
    35. TERT expression was up-regulated by a histone deacetylase inhibitor, while the induction of TERT in lung fibroblasts was associated with the binding of acetylated histone H3K9 to the TERT promoter region. PMID: 23526226
    36. calorie restriction attenuates telomere erosion associated to aging and that synergizes with TERT over-expression in increasing "health span" and extending mouse longevity PMID: 23349740
    37. prepared two mutant forms of the PhSurv-PhTERT tandem with two or four Sp1 sites removed from the distal "long" PhSurv promoter PMID: 23056318
    38. Study identified the cells responsible for cardiac telomerase activity, demonstrates a significant diminution with age. PMID: 22919071
    39. mir498 has a role in 1,25-Dihydroxyvitamin D3 suppression of telomerase expression and cancer growth PMID: 23055531
    40. Data show that AUF1 binds and strongly activates the transcription promoter for telomerase catalytic subunit Tert. PMID: 22633954
    41. Essential role for telomerase in chronic myeloid leukemia induced by BCR-ABL in mice. PMID: 22408137
    42. telomerase plays a role in the aging of nondividing cells PMID: 22533433
    43. findings show beta-catenin regulates Tert expression through interaction with Klf4, a core component of the pluripotency transcriptional network; beta-Catenin binds to the Tert promoter in a mouse intestinal tumor model PMID: 22723415
    44. Data provide evidence that telomere dysfunction plays a critical role in prostate cancer initiation and progression, permitting acquisition of and selection for cancer-relevant genomic events upon telomerase reactivation. PMID: 22341455
    45. Silencing transgenic TERT expression or inhibiting Wnt signaling through systemic expression of the Wnt inhibitor Dkk1 in either TERT transgenic mice or in a mouse model of HIVAN results in marked normalization of podocytes PMID: 22138751
    46. Selenium and benzene can upregulate the telomerase activity in mouse lymphocytes in vivo. PMID: 19080380
    47. Hippocampal telomerase is involved in the modulation of depression-related behaviors, possibly by regulating adult neurogenesis. PMID: 21865469
    48. Primitive hematopoietic Tert(-/-) cells lacking telomerase activity exhibit signs of enhanced DNA damage. PMID: 21730353
    49. Results indicate that both telomerase reverse transcriptase and telomerase RNA are haploinsufficient and that their deficiency leads to telomere shortening, which limits tissue renewal. PMID: 21464209
    50. The results of this study provide the first experimental evidence that telomere shortening, despite impairing adult neurogenesis and maintenance of post-mitotic neurons, can slow down the progression of amyloid plaque pathology in Alzheimer's disease. PMID: 21672962

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body.
  • 蛋白家族：
    Reverse transcriptase family, Telomerase subfamily
  • 组织特异性：
    High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.
  • 数据库链接：

    KEGG: mmu:21752

    STRING: 10090.ENSMUSP00000022104

    UniGene: Mm.10109