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  4. Recombinant Mouse Telomeric repeat-binding factor 2 (Terf2)

Recombinant Mouse Telomeric repeat-binding factor 2 (Terf2)

  • 货号：
    CSB-YP023388MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023388MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023388MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023388MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023388MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Terf2; Trf2; Telomeric repeat-binding factor 2; TTAGGG repeat-binding factor 2; Telomeric DNA-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-541
  • 氨基酸序列
    MAAGAGTAGP ASGPGVVRDP MASQPRKRPS REGGEGGEGE RRSNTMAGGG GSSDSSGRAA SRRASRSGGR ARRGRHEPGL GGAAERGAGE ARLEEAVNRW VLKFYFHEAL RAFRSSRYRD FRQIRDIMQA LLVRPLGKEH TVSRLLRVMQ CLSRIEEGEN LDCSFDMEAE LTPLESAINV LEMIKTEFTL TDSMVESSRK LVKEAAVIIC IKNKEFEKAS KILKKYMSKD PTTQKLRTDL LNIIREKNLA HPVIQNFSYE VFQQKMLRFL ESHLDDTEPY LLTMAKKALK SESAASSTMR EEKHPEPVEK PLREPPSRQP QNPPATIGIR TLKAAFKALS TAQDSEAAFA KLDQKDLVLA NLASPSSPAH KHKRPRKDEH ESAAPAEGEG GSDRQPRNSP MTISRLLLEE DSQSTEPSPG LNSSHKAMSA SKPRALNQPH PGEKKPKASK DKWNSPNGLE EKEVWLEEDQ LFEVQAPGED RSSSLTRKQK WTIEESEWVK DGVRKYGEGN WAAISKSYPF VNRTAVMIKD RWRTMKKLGM N
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • 基因功能参考文献：
    1. shelterin subunits exhibit distinct spatiotemporal expression patterns, suggesting important tissue-specific functions during development and aging PMID: 28437249
    2. Selective inactivation of Terf2 in neural progenitors induced apoptosis, resulting in a complete loss of the brain structure. PMID: 28620865
    3. The s propose that TRF2 masks and stabilizes the t-loop three-way junction, thereby protecting telomeres from detrimental deletions and PARP1 activation. PMID: 28805810
    4. TRF2, a component of shelterin, binds to core histones to protect chromosome ends from inappropriate DNA damage response and loss of telomeric DNA. The N-terminal Gly/Arg-rich domain (GAR domain) of TRF2 directly binds to the globular domain of core histones. PMID: 27514743
    5. Overexpressing TRF2-S and silencing FMRP promotes mRNA entry to axons and enhances axonal outgrowth and neurotransmitter release from presynaptic terminals. PMID: 26586091
    6. TRF2 binds and transactivates the promoter of the angiogenic tyrosine kinase platelet-derived growth factor receptor beta (PDGFRbeta). PMID: 25437559
    7. role of TRF2 in skin homeostasis, is reported. PMID: 24725274
    8. shelterin protein TIN2 can protect chromosome ends as a TRF2-tethered TIN2/TPP1/POT1 complex that lacks a physical connection to TRF1 PMID: 24469404
    9. We concluded that genomic instability resulting from loss of TRF2 expression provides biological advantages to the cancer stem cell population PMID: 23178498
    10. Conditional deletion of individual components of shelterin showed that TRF2 was required for the formation and/or maintenance of t-loops, whereas deletion of TRF1, Rap1, or the POT1 proteins (POT1a and POT1b) had no effect on the frequency of t-loop occurrence. PMID: 24120135
    11. TAF7L associates with TRF2 both in vitro and in testis, suggesting that TAF7L likely cooperates directly with TRF2 at promoters of a subset of postmeiotic genes to regulate spermiogenesis. PMID: 24082143
    12. Experiments in cells from knockout mice showed that uPAR controls the ubiquitin-proteasome system in VSMC & regulates doxorubicin-induced TRF2 ubiquitination and proteasomal degradation via this mechanism. PMID: 23172421
    13. the molecular properties of TRF2 that are both necessary and sufficient to protect chromosome ends in mouse embryonic fibroblasts: the data support a two-step mechanism for TRF2-mediated end protection PMID: 23389450
    14. TRF2 provides a crucial link between telomere function and ultraviolet-induced damage repair, whose alteration underlies genomic instability, cancer and aging. PMID: 16142233
    15. PARP1 associates with telomere repeat binding factor 2 (TRF2) and is capable of poly(ADP-ribosyl)ation of TRF2, which affects binding of TRF2 to telomeric DNA. PMID: 16436506
    16. with overexpressing the TRF2 telomere-binding protein, mice show short telomeres in the presence of telomerase activity, leading to premature aging and increased cancer PMID: 16582635
    17. TRF2 and POT1 act independently to repress two DNA damage response pathways; TRF2 represses ATM, whereas POT1 prevents activation of ATR PMID: 17687332
    18. The inactivation of TRF2ts at 37 degrees C was rapid and reversible, permitting induction of short periods (3-6 h) of telomere dysfunction in the G0, G1, and S/G2 phases of the cell cycle. PMID: 18451109
    19. These findings indicate a previously unnoticed role for TRF2 in the assembly of telomeric chromatin. PMID: 18971622
    20. the TRF2 telomeric protein has a role in telomere-associated diseases in mouse epidermal stem cells PMID: 19259387

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere. Note=Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • 数据库链接：

    KEGG: mmu:21750

    STRING: 10090.ENSMUSP00000118759

    UniGene: Mm.6402