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  4. Recombinant Mouse Thrombopoietin receptor (Mpl)

Recombinant Mouse Thrombopoietin receptor (Mpl)

  • 货号：
    CSB-YP600093MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP600093MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP600093MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP600093MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP600093MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mpl; Tpor; Thrombopoietin receptor; TPO-R; Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; CD antigen CD110
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Extracellular domain
  • 表达区域：
    26-482
  • 氨基酸序列
    QDVFLLALGTEPLNCFSQTFEDLTCFWDEEEAAPSGTYQLLYAYRGEKPRACPLYSQSVP TFGTRYVCQFPAQDEVRLFFPLHLWVKNVSLNQTLIQRVLFVDSVGLPAPPRVIKARGGS QPGELQIHWEAPAPEISDFLRHELRYGPTDSSNATAPSVIQLLSTETCCPTLWMPNPVPV LDQPPCVHPTASQPHGPAPFLTVKGGSCLVSGLQAGKSYWLQLRSQPDGVSLRGSWGPWS FPVTVDLPGDAVTIGLQCFTLDLKMVTCQWQQQDRTSSQGFFRHSRTRCCPTDRDPTWEK CEEEEPRPGSQPALVSRCHFKSRNDSVIHILVEVTTAQGAVHSYLGSPFWIHQAVLLPTP SLHWREVSSGRLELEWQHQSSWAAQETCYQLRYTGEGREDWKVLEPSLGARGGTLELRPR ARYSLQLRARLNGPTYQGPWSAWSPPARVSTGSETAW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.; Acts as an inhibitor of thrombopoietin signaling by promoting protein down-regulation of full-length isoform Mpl-fl.
  • 基因功能参考文献：
    1. these results demonstrate that MPL P106L is a receptor with an incomplete defect in trafficking. PMID: 28034873
    2. C-Mpl is expressed on osteoblasts and osteoclasts and is important in regulating skeletal homeostasis. PMID: 26375403
    3. CALR mutants are sufficient to induce thrombocytosis through MPL activation. PMID: 26608331
    4. Thrombopoietin receptor activation by myeloproliferative neoplasm associated calreticulin mutants. PMID: 26668133
    5. The interaction between Mpl and Atp5d was confirmed by the yeast two-hybrid system, mammalian two-hybrid assay, pull-down experiment, and co-immunoprecipitation study in vivo and in vitro. PMID: 24615392
    6. Mouse prenatal platelet-forming lineages share a core transcriptional program but divergent dependence on MPL. PMID: 25995324
    7. MERIT40 deficiency triggers hypersensitivity to Tpo stimulation and the stem cell phenotypes are abrogated on a background null for the Tpo receptor Mpl. PMID: 25636339
    8. OTT1 regulates the alternative splicing of Mpl-TR, a truncated isoform of c-Mpl, which modulates Thrombopoietin-mediated signaling. PMID: 25468569
    9. These studies show the importance of the c-Mpl membrane-distal cytokine receptor homology module to thrombopoietin-binding and suggest that mutations within this CRM that perturb TPO binding could give rise to Congenital Amegakaryocytic Thrombocytopenia. PMID: 24438083
    10. Mpl expression on megakaryocytes and platelets is essential to prevent megakaryocytosis and myeloproliferation. PMID: 24711413
    11. These experiments define a novel VEGF-miR-1-Mpl-P-selectin effector pathway in lung Th2 inflammation and herald the utility of miR-1 and Mpl as potential therapeutic targets for asthma. PMID: 24043765
    12. Gene-corrected Mpl(-/-) cells had increased long-term repopulating potential, with a marked increase in lineage(-)Sca1(+)cKit(+) cells and early progenitor populations in reconstituted mice. PMID: 21289307
    13. Data indicate that Mpl is an active player during the first steps of definitive hematopoiesis establishment through direct regulation of the expression of transcription factors or genes important for the self-renewal, proliferation and apoptosis of HSCs. PMID: 20711984
    14. Data locate Mpl expression to neurons at different subdivisions of the spinal cord, rhombencephalon, midbrain and prosencephalon; besides neuronal cells Mpl protein is also expressed in Purkinje cells of the adult cerebellum. PMID: 20667107
    15. study describes a link between Mpl receptor signaling and IKK and NF-kappaB activities in megakaryocytes PMID: 11967992
    16. mpl-II isoform cloning, base sequence, and amino acid sequence - lacks a 60 amino acid region required for surface expression of the protein PMID: 11997107
    17. Thrombopoietin mediates cell proliferation in cells expressing high levels of Mpl but only mediates survival without proliferation in cells expressing low levels of the receptor. PMID: 12006617
    18. A 2-kb c-mpl promoter fragment is sufficient to direct expression to the megakaryocytic lineage and sites of embryonic hematopoiesis in transgenic mice. PMID: 12130527
    19. receptor motifs involved in TPO-induced internalization of Mpl are identified; results suggest that Mpl translocation may be necessary for normal cellular proliferation and that a pool of intracellular Mpl can be rapidly recycled to the cell surface PMID: 12623841
    20. c-Mpl(-/-) Kit(Wv/Wv) mice reveal nonredundant and synergistic effects of Tpo and steel factor on primitive hematopoietic cells. PMID: 15138166
    21. c-Mpl truncated isoform with an essential C-terminal peptide is required for a proteolytic process PMID: 15210714
    22. mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
    23. studies suggest that c-Mpl does not control HSC numbers, as had been previously argued, but rather facilitates the early expansion of differentiating clones. PMID: 17347409
    24. Dual role of Mpl in the generation and expansion of hematopoietic stem cells during establishment of definitive hematopoiesis. PMID: 17634189
    25. Down-regulation of Mpl marks the transition to lymphoid-primed multipotent progenitors with gradual loss of granulocyte-monocyte potential PMID: 18218856
    26. c-Myb/p300 transcriptional regulatory complex has an indispensable role in megakaryocyte development and suppresses thrombocytopenia in Mpl-/- mice PMID: 18684867
    27. AML1 can regulate the c-mpl promoter both positively and negatively by changing the binding partner according to cell types PMID: 18687690
    28. impaired TPO(thrombopoietin) homeostasis in the Mpl transgene-rescued mice produces elevated plasma TPO levels, which serves as an unchecked stimulus to drive the observed excessive megakaryocytopoiesis PMID: 18796624
    29. the severe reduction of Mpl protein in platelets in Mpl-transgenic Mpl(-/-) mice shifts the equilibrium of this feedback loop, resulting in markedly elevated levels of megakaryocytes and platelets at steady state PMID: 18845793
    30. Thrombocytopenia in HLB219 mice is caused by a recessive mutation in Mpl that abrogates mitogen-activated protein kinase-extracellular signal regulated kinase and janus kinase-signal transducers and activators of transcription signaling. PMID: 19059699

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cell surface.
  • 蛋白家族：
    Type I cytokine receptor family, Type 1 subfamily
  • 数据库链接：

    KEGG: mmu:17480

    STRING: 10090.ENSMUSP00000130167

    UniGene: Mm.4864