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  4. Recombinant Mouse Toll/interleukin-1 receptor domain-containing adapter protein (Tirap)

Recombinant Mouse Toll/interleukin-1 receptor domain-containing adapter protein (Tirap)

  • 货号：
    CSB-YP860800MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP860800MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP860800MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP860800MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP860800MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tirap; Mal; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-241
  • 氨基酸序列
    MASSSSVPAS STPSKKPRDK IADWFRQALL KKPKKMPISQ ESHLYDGSQT ATQDGLSPSS CSSPPSHSSP ESRSSPSSCS SGMSPTSPPT HVDSSSSSSG RWSKDYDVCV CHSEEDLEAA QELVSYLEGS QASLRCFLQL RDAAPGGAIV SELCQALSRS HCRALLITPG FLRDPWCKYQ MLQALTEAPA SEGCTIPLLS GLSRAAYPPE LRFMYYVDGR GKDGGFYQVK EAVIHYLETL S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter involved in the TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
  • 基因功能参考文献：
    1. TIARP independently down-regulated CXCL2 and IL-6 production by fibroblast-like synoviocytes, and the expression of chemokine receptors (CXCR1 and CXCR2) in neutrophils, with resultant reduction of neutrophil migration into arthritic joints. PMID: 27995997
    2. Our key findings provide novel insights into the mechanism of action of heterotrimeric complex (PKCdelta-TIRAP-p38) in proinflammatory cytokine expression, which controls the development of the inflammatory trigger in stimulated macrophages. PMID: 28528205
    3. Results suggest that TIR domain-containing adaptor protein positively regulated BV2 microglial M1 polarization through toll-like receptor 4 -mediated Transforming Growth Factor-Beta-Activated Kinase 1/I-Kappa-B Kinase /Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B-Cells (TAK1/IKK/NF-kappaB), mitogen-activated protein kinases and Akt signalling pathways. PMID: 27061018
    4. The adapter Mal (encoded by TIRAP) has appeared crucial for the cytokine production by Ly6C(lo) but not by Ly6C(hi) monocytes. The protein Mal was necessary to induce cytokine synthesis by Ly6C(lo) monocytes after triggering TLR2 or TLR9. PMID: 27646001
    5. genetic polymorphism is associated with interferon-gamma signaling and susceptibility to infections PMID: 26885859
    6. TIRAP/Mal deficiency significantly inhibited the activity of titanium particles with adherent bacterial debris to stimulate in vivo osteolysis and in vitro cytokine mRNAs and secretion. PMID: 26888676
    7. A critical role for the TLR signaling adapter Mal in alveolar macrophage-mediated protection against Bordetella pertussis. PMID: 25515629
    8. MyD88 adaptor-like (Mal) functions in the epithelial barrier and contributes to intestinal integrity via protein kinase C. PMID: 23612054
    9. our data reveal a differential, but inflammation-independent, requirement for Mal and MyD88 during TLR2-promoted gastric tumourigenesis. PMID: 23728346
    10. Mal is essential for the maintenance of intestinal homeostasis and expression of Mal in nonhematopoietic cells prevents chronic intestinal inflammation that may predispose to colon neoplasia. PMID: 24603458
    11. Study reports that, in response to natural activators of innate immunity, the sorting adaptor TIRAP regulates TLR signaling from the plasma membrane and endosomes. PMID: 24529375
    12. TIRAP plays a central role in regulating the effects of lipopolysaccharides or lipoteichoic acid on chlorpromazine-induced hepatotoxicity. PMID: 23238562
    13. PIP5Kalpha promotes TLR4-associated microglial inflammation by mediating PIP(2)-dependent recruitment of TIRAP to the plasma membrane PMID: 23297396
    14. PTEN deletion impeded Mal localization at the plasma membrane and suppressed Mal-TLR5 interaction. These results suggest that, by controlling Mal recruitment, PTEN regulates TLR5-induced inflammatory responses. PMID: 23038756
    15. Toll-like receptor signal adaptor protein MyD88 is required for sustained endotoxin-induced acute hypoferremic response in mice. PMID: 22497726
    16. findings support that ozone-induced airway hyperresponsiveness is dependent on the HA-TLR4-MyD88-TIRAP signaling pathway PMID: 22073274
    17. the effect of TLR2 on drug-metabolizing enzyme genes in hepatocytes was mediated by TIRAP, whereas TIRAP was not involved in mediating the effects of TLR2 on cytokine expression in the liver PMID: 21303924
    18. Long-term exposure of murine airway to IL-1beta induces up- and down-regulation of mRNA expression for Toll-IL-1 receptor signal molecules.[Toll-IL-1 receptor] PMID: 19825525
    19. The cardioprotective effect of ischemic preconditioning is mediated, at least in part, through a TLR2-TIRAP-dependent pathway. The modulation of this pathway represents a viable target for reducing ischemia-reperfusion injury. PMID: 20061547
    20. Phagosomal retention of Francisella tularensis results in TIRAP/Mal-independent TLR2 signaling. PMID: 19889726
    21. The adapter MyD88-adapter-like (Mal) is recruited specifically by TLR-4. The set of genes being induced may be tailored for the subsequent elimination of the pathogen being recognized, due to differences in signal transduction pathways activated by TLRs. PMID: 12072368
    22. The induction of IL-6 gene expression in macrophages via Tlr4 is TIRAP-dependent. PMID: 12421970
    23. TIRAP has a crucial role in the MyD88-dependent signalling pathway shared by TLR2 and TLR4. PMID: 12447441
    24. TIRAP is differentially involved in signalling by members of the TLR family and may account for specificity in the downstream signalling of individual TLRs PMID: 12447442
    25. Removal of SOCS-1 regulation potentiates Mal-dependent p65 phosphorylation and transactivation of NF-kappaB, leading to amplified inflammatory responses. PMID: 16415872
    26. Novel findings illustrate a pathogen-specific role for TIRAP in mediating pulmonary defense in the lung against Klebsiella pneumoniae, but not Pseudomonas aeruginosa. PMID: 16785551
    27. LCMV infection of glial cells up-regulated TLR2, MHC class-I & II, CD40, CD86 in a MyD88/Mal-dependent manner. TLR signaling participates in viral-infection-induced activation of CNS glial cells & the immunopathology in the CNS. PMID: 18295350
    28. the novel role for Mal in facilitating the direct recruitment of TRAF6 to the plasma membrane, which is necessary for TLR2- and TLR4-induced transactivation of NF-kappaB and regulation of the subsequent pro-inflammatory response. PMID: 19592497
    29. tIRAP was inhibitory for TLR3-dependent IL-6 and JNK induction PMID: 19717524

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Membrane.
  • 数据库链接：

    KEGG: mmu:117149

    STRING: 10090.ENSMUSP00000135224

    UniGene: Mm.23987