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  4. Recombinant Mouse Toll-like receptor 4 (Tlr4), partial

Recombinant Mouse Toll-like receptor 4 (Tlr4), partial

  • 货号：
    CSB-YP023603MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tlr4; Lps; Toll-like receptor 4; EC 3.2.2.6; CD antigen CD284
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    71.5kDa
  • 表达区域：
    26-638aa
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The sequence encoding the 26-638aa of the Mouse Toll-like receptor 4 (Tlr4) was expressed in the yeast. Each product has fused a 6xHis-tag at the N-terminus for purification. The recombinant truncated mouse Tlr4 protein was purified by SDS-PAGE and got a purity of over 90%. The Tlr4 protein migrated to a molecular weight of about 75 kDa on the reducing SDS-PAGE gel. In addition to specific antibody production, this protein may be used in the immunological studies involved in Tlr4.

    Tlr4 is a major pathogen recognition receptor (PRR) expressed on the cell surface of several immune cells including macrophages and dendritic cells. It is the exclusive family member that signals through both MYD88-dependent and MYD88-independent, TRIF-dependent pathways. Tlr4 signalings mediated via the above two manners are involved in the production of proinflammatory cytokines and the induction of the full innate immune response, respectively. Besides, Tlr4 is also associated with the pathogenesis of pro-inflammatory based diseases such as multiple sclerosis (MS).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

我们需要大约 2 到 5mg蛋白质，无载体和无内毒素的，用于体内实验。

A:
carrier-free是指不含BSA，我们的蛋白都是不含的； 我们可以免费提供内毒素去除服务，使得内毒素含量小于1.0 EU/ug； 如果您用于体内实验，推荐我们免费的无菌和内毒素去除服务。

靶点详情

  • 功能：
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation.
  • 基因功能参考文献：
    1. intestinal epithelial TLR4 regulates metabolic syndrome through altered host-bacterial signaling PMID: 29363671
    2. These data suggest that targeting TLR TIR domains may provide novel pharmacological targets to reduce or reverse TLR4-dependent pain behavior in the rodent. PMID: 28623271
    3. We further show that EGFR is activated through toll-like receptor 4. Disruption of toll-like receptor 4 or the EGFR pathway led to reduced inflammatory activity and foam cell formation PMID: 28374780
    4. Bone marrow malfunction arises early in obesity and depends on precursor-intrinsic TLR4. PMID: 29453396
    5. Toxoplasma gondii ROP38 promotes the maturation of dendritic cells mediated by TLR4. PMID: 29773099
    6. Local inhibition of TLR4 and MyD88 might reduce immune responses and ameliorate allograft rejection. PMID: 28393847
    7. Results concluded that a hyperactive TLR4-NF-kappaB signal and higher level of cytokines are involved in susceptibility to depression in stressed obese mice. PMID: 29765402
    8. these data showed that TLR4 knockout ameliorated high fat diet-induced cardiac contractile and intracellular Ca(2+) anomalies through inhibition of inflammation and ROS, possibly through a NF-kappaB/JNK-dependent activation of autophagy. PMID: 28108421
    9. miR-711, which is upregulated by Adipoq, represses TLR4 signaling, acting therefore as a major mediator of the anti-inflammatory action of Adipoq. PMID: 28240307
    10. TLR4 expression in placenta is up-regulated during maternal murine cytomegalovirus infection. PMID: 30128872
    11. Loss of TLR4 in the mouse retinal Muller cells impaired insulin signal transduction. PMID: 29490521
    12. TREM-1 may play an important role together with TLR4 in the nasopharyngeal clearance of Haemophilus influenzae by neutrophils. PMID: 30055735
    13. TLR4-mediated p62 autophagic impairment plays an important role in the occurrence and development of neuropathic pain. PMID: 29486776
    14. atorvastatin treatment may protect BV2 microglia and hippocampal neurons from oxygenglucose deprivationinduced neuronal inflammatory injury by suppressing the TLR4/TRAF6/NFkappaB pathway. This may provide a potential strategy for the treatment of neuronal injury. PMID: 29845194
    15. these results suggest that TanIIA treatment is beneficial for improvement of Hypoxic ischemic encephalopathy through TLR4mediated NFkappaB signaling. PMID: 29956801
    16. Both pharmacologic inhibition and genetic knockout of TLR4 completely abolished mesenteric lymph (ML) exosome-induced cytokine production in macrophages. Our findings define the critical role of exosomes secreted into ML as a critical mediator of trauma/hemorrhagic shock-induced acute lung injury through macrophage TLR4 activation. PMID: 28855278
    17. Fusobacterium nucleatum infection increased M2 polarization and intestinal tumour growth in ApcMin/+ mice and was TLR4dependent. PMID: 30121899
    18. TLR4 plays a regulatory role in the type 1 immune responses during C. sinensis infection, controlling release of Th1/Th2 cytokines by infected splenocytes and dendritic cells. PMID: 29047038
    19. This study demonstrates that the synergistic effect between TLR4 and TLR3 in macrophages is an important determinant in acute lung injury and, more importantly, that TLR3 up-regulation is dependent on TLR4-MyD88-NF-kappaB signaling. PMID: 28198368
    20. Toll-like receptor 4 (TLR4) requires physical and functional association with Fcalpha-mu protein (Fcalpha/muR) for its oligomer formation and interleukin-6 (IL-6) production from marginal zone (MZ) B cells. PMID: 27146354
    21. The expression of Toll-like receptor (TLR)4 and NF-kappaB p50 was also inhibited by Andro. Additionally, in vitro data confirmed that Andro treatment not only attenuated the expression of profibrotic and proinflammatory factors but also blocked the TGF-beta1/Smad2 and TLR4/NF-kappaB p50 pathways. PMID: 29743985
    22. CatH deficiency impaired toll-like receptor 3-mediated activation of interferon regulatory factor 3 and consequent secretion of IFN-beta PMID: 29470604
    23. TLR4(-/-) mice showed massive alpha-SYN protein accumulation and dopaminergic cell loss in substantia nigra. PMID: 29589201
    24. The results suggested that dioscin prevents LPSinduced ALI through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70. PMID: 29512786
    25. this study shows that endocytosis of particulate matter induces cytokine production by neutrophil via Toll-like receptor 4 PMID: 29522983
    26. Study showed the significance of TLR4 in upregulation of MAPK/ErK in NMDAR hypofunction schizophrenic-mouse model. This is characterized by a decrease in hippocampal IGF-1R/CaMKIIalpha, and social interaction behavioral deficits. Ultimately, hippocampal TLR4 knockdown rescued IGF-1R/CaMKIIalpha decline and prevented behavioral deficits by reducing MAPK/ErK expression. PMID: 29137928
    27. Study highlights the importance of Ca(2+) signaling in macrophage activation and identifies the ion channel TRPM7 as a central component of TLR4 signaling. PMID: 29343440
    28. The immunoenhancement effect of PSP against lung cancer is mediated by TLR4-MAPK/NF-kappaB signaling pathways PMID: 29343453
    29. knockout of TLR4 gene improved survival and cardiac function in severe sepsis induced by CLP by decreasing the myocardial levels of inflammatory cytokines, weakening neutrophil infiltration in myocardium, and attenuating the heart apoptosis. PMID: 29393431
    30. beta-arrestin 2 can protect liver tissue from LPS-induced injury via inhibition of TLR4/NF-kappaB signaling pathway-mediated inflammation. PMID: 29375207
    31. TLR4 mutation can decrease the expression of inflammatory factors and enhance the speed of recovery after sciatic nerve injury. PMID: 29227824
    32. Toll-like receptor (TLR) 4, the expression of which was upregulated in B cells after ultraviolet B exposure, played an essential role in the induction of regulatory B cells. PMID: 29205369
    33. Pudilan xiaoyan oral liquid prevents LPS-induced respiratory in fl ammation via effects on TLR4/NF-kappaB signaling. PMID: 28689797
    34. NOD2 Modulates Serotonin Transporter and Interacts with TLR2 and TLR4 in Intestinal Epithelial Cells PMID: 29913461
    35. The results demonstrated prolific RSV infection of N2a cells, which triggered a decrease of NeuN protein expression, coinciding with an increase of nuclear lesions, F protein expression, RSV viral titers, and late apoptotic levels of N2a cells. RSV infection induced co-localization of RSV F protein with TLR4 and nucleolin. PMID: 29427996
    36. These data suggest that neutrophil extracellular traps are generated in ventilator-induced lung injury (VILI) and pathogenic in a mouse model of VILI, and their formation is partially dependent on TLR4. PMID: 29387725
    37. this study shows that Toll-like receptor 4 deficiency increases resistance in sepsis-induced immune dysfunction PMID: 29149705
    38. In conclusion, the data support that TLR4 is involved in the insulin signaling pathway in the retina. PMID: 28681194
    39. this study shows that lipopolysaccharide)-mediated priming of TLR4 enhances oxidant-induced prostaglandin E2 biosynthesis in primary murine macrophages PMID: 29161659
    40. bone marrow transplantation between wild-type and TLR4 knockout mice revealed that hepatic TLR4-dependent hepcidin expression was comparable to macrophage TLR4-dependent hepcidin expression induced by LPS PMID: 29217822
    41. this study reveals a novel mechanism of naringenin through AMPK-ATF3-dependent negative regulation of the LPS/TLR4 signalling pathway, which thereby confers protection against murine endotoxaemia. PMID: 28004841
    42. These data demonstrate that the Pb18 strain of Paracoccidioides brasiliensis is able to activate the transcription of Notch1 receptor in J774 macrophages. Activation of this receptor with also activation of TLR 4 (via LPS) induces IL-6 production, which favors the pathogenesis. PMID: 28600728
    43. In human resistin-expressing transgenic mice and resistin prevents lipopolysaccharide (LPS)-induced mortality by blocking LPS binding to its receptor Toll-like receptor 4 (TLR4) and by promoting anti-inflammatory signaling. PMID: 29133417
    44. The findings of this study indicated that activation of the neuroimmune system and TLR4 signaling may possibly be involved in environmental pollutant-induced spatial learning impairment in neonatal mice. PMID: 29107071
    45. Ts-Hsp70 elicited protective immunity against T. spiralis infection was reduced in TLR2-/- and TLR4-/- mice correlating with the reduced humoral and cellular immune responses. PMID: 29775453
    46. Rosiglitazone Regulates TLR4 and Rescues HO-1 and NRF2 Expression in Myometrial and Decidual Macrophages in Inflammation-Induced Preterm Birth. PMID: 28322133
    47. TLR4 and NLRP3 are suppressed by alpinectin, which attenuates inflammation in DSS-induced acute colitis PMID: 27321991
    48. Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE-knockout mice by activation of TLR4. PMID: 28849148
    49. Study shows that the disulfide form of high mobility group box-1 mediates bladder pain directly (not secondary to inflammation or injury) through activation of toll-like receptor 4 receptors in the bladder. PMID: 28545586
    50. ablation of TLR4 mitigates cardiac mitochondrial dysfunction in a model of HHcy PMID: 28738166

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  • 相关疾病：
    The protein is encoded by the Lps locus, an important susceptibility locus, influencing the propensity to develop a disseminated Gram-negative infection.
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Early endosome. Cell projection, ruffle.
  • 蛋白家族：
    Toll-like receptor family
  • 组织特异性：
    Expressed in macrophages (at protein level). Highly expressed in heart, spleen, lung and muscle. Lower levels are found in liver and kidney.
  • 数据库链接：

    KEGG: mmu:21898

    STRING: 10090.ENSMUSP00000045770

    UniGene: Mm.38049