Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Trans-acting T-cell-specific transcription factor GATA-3 (Gata3)

Recombinant Mouse Trans-acting T-cell-specific transcription factor GATA-3 (Gata3)

  • 中文名称：
    小鼠Gata3重组蛋白
  • 货号：
    CSB-YP009276MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gata3重组蛋白
  • 货号：
    CSB-EP009276MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gata3重组蛋白
  • 货号：
    CSB-EP009276MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gata3重组蛋白
  • 货号：
    CSB-BP009276MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gata3重组蛋白
  • 货号：
    CSB-MP009276MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gata3; Gata-3Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-443
  • 氨基酸序列
    MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression.
  • 基因功能参考文献：
    1. The enhanced Th1 and Th2 immune response in K + O immunized mice was also supported by the increased expression of Tbx21 and GATA-3 transcription factors in splenocytes. This corroborated with increased BLIMP-1 and Oct-2 protein expression. PMID: 29486619
    2. Transcription factor GATA3 is responsible for the ER stress-induced TRIP-Br2 expression in visceral fat. PMID: 27109496
    3. We have identified ZPO2 as a negative regulator of GATA3, thus providing an alternative mechanism that results in a reduction in or perhaps even loss of GATA3 during breast cancer development. PMID: 28258171
    4. Findings suggest that VEGF gene expression can be suppressed by TNFSF15-stimulated activation of the JNK-GATA3 signaling pathway which gives rise to up-regulation of miR-29b. PMID: 27589684
    5. RHS6 is a critical regulatory element for allergic airway inflammation and for coordinate regulation of Th2 cytokine genes by recruiting GATA3, SATB1, and IRF4. PMID: 27878828
    6. the model of mutually antagonistic differentiation programs driven by mutually exclusively expressed T-bet or GATA-3 does not completely explain natural CD4 T cell priming outcomes PMID: 29088218
    7. MIF-deficient mice have reduced Nippostrongylus brasiliensis burden and mounted an enhanced type 2 immune response, including increased Gata3 expression and IL-13 production in the mesenteric lymph nodes PMID: 27049059
    8. study demonstrates a role for HOX5 proteins in controlling lung inflammation during a model of allergic asthma. The increased inflammation in the Hox5-deficient mice was mediated by increased Th2 cell development, which was associated with regulation of Gata3 expression PMID: 28576978
    9. Treg cell accumulation in intestinal tumours from APC(min/+) mice was exclusively due to the increase in KLRG1(+) GATA3(+) Treg cells. PMID: 28437001
    10. GATA3 abundance regulates the recombination propensity at the Tcrb locus. PMID: 28320875
    11. priming of T helper cells by IL-6-deficient antigen-presenting dendritic cells preferentially leads to accumulation of a subset of Follicular helper T cells characterized by high expression of GATA3 and IL-4. PMID: 27474166
    12. Gata3 was first expressed in all developing neurons, followed by a decrease in type I primary auditory neurons as they matured. PMID: 28118374
    13. Mediated by GATA3 through targeting promoter region of ORMDL3. PMID: 28031110
    14. these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias. PMID: 27870920
    15. Results provide evidence for a critical role for GATA3 in the maintenance of mesangial cells and its absolute requirement for prevention of glomerular disease. PMID: 27296697
    16. It is proposed that the interactions between ANXA1 and GATA-3 may provide clues to understand the immunosuppression and have potential as new therapeutic targets in immunotherapy after sepsis. PMID: 27833268
    17. GATA3 is expressed in both prostate basal progenitor and luminal cells and loss of GATA3 leads to a mislocalization of PRKCZ, resulting in mitotic spindle randomization during progenitor cell division. PMID: 28285879
    18. expression of wild type GATA-3 activated OPG mRNA and protein expression, while the expression of a dominant negative mutant of GATA-3 or a GATA-3 shRNA construct reduced OPG mRNA and protein levels PMID: 26216189
    19. The Tce1 enhancer is both necessary and sufficient for critical aspects of Gata3 T cell-specific transcriptional activity. PMID: 26808502
    20. These results suggest that Oct-1 and GATA-3/Oct-2 direct Th2 cytokine gene expression in a cooperative manner. PMID: 26840450
    21. Cytokines analysis in peritoneal fluid showed that IFN-gamma was significantly increased in chlorhexidine gluconate (CG)-injected T-bet Tg mice and that IL-13 was significantly increased in CG-injected GATA-3 Tg mice PMID: 26156402
    22. our results demonstrate that GATA3 is a critical regulator for NK cell terminal maturation and egress out of the bone marrow PMID: 26773150
    23. Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment. PMID: 26595886
    24. Data indicate that overexpression of micrRNA miR-135a alters transcription factor T-bet and GATA binding protein 3 (GATA-3) mRNA expression in allergic rhinitis (AR). PMID: 26418311
    25. PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells PMID: 26361334
    26. The results of the present study suggested that the therapeutic mechanism by which afzelin effectively treats asthma is based on reduction of Th2 cytokine via inhibition of GATA-binding protein 3 transcription factor. PMID: 25738969
    27. that loss of one Gata3 allele leads to diminished expansion (and compromised development) of immature T cells as well as aberrant induction of myeloid transcription factor PU.1. PMID: 26385963
    28. We herein identified a mechanism whereby the methylation of Gata3 at Arg-261 regulates the transcriptional activation of the Il5 gene in Th2 cells. PMID: 25861992
    29. Itm2a is a target gene of GATA-3 that plays a minimal role in regulating the development and function of T cells PMID: 24831988
    30. Data propose that control of GATA3 levels by Fbw7 contributes to the fine-tuning of T-cell development. PMID: 24820417
    31. miR-183, though negatively regulated by Gata3, enhances 3T3-L1 preadipocyte differentiation and adipogenesis by targeting LRP6. PMID: 24556500
    32. the pattern of GATA-3 expression sequentially produces B lineage exclusion, T lineage progression, and myeloid-lineage exclusion for commitment. PMID: 25172496
    33. Inhibition of GATA3 activity or blockade of GATA3 expression may attenuate the interleukin-13 mediated asthma phenotypes. PMID: 24363163
    34. role for Nkx2-5 and Gata3 in the ontogeny of the smooth muscle gastric ligaments; gastric ligaments coexpress Gata3, Nkx2-5 and Sox9; germline loss of Gata3 or conditional deletion of Nkx2-5 abrogates Sox9 expression and impairs gastric ligament smooth muscle development PMID: 24970776
    35. GATA-3 overexpression in T-cells and IL-13 might play important roles in the development of dextran sulfate sodium-induced colitis. PMID: 24770638
    36. Mechanistically, JARID1B was required for GATA3 recruitment to the Foxa1 promoter to activate Foxa1 expression. PMID: 24802759
    37. This study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. PMID: 24674670
    38. The transcription factor GATA3 is critical for the development of all IL-7Ralpha-expressing innate lymphoid cells. PMID: 24631153
    39. Gata3 plays a generalized role in innate lymphoid cell (ILC) lineage determination and is critical for the development of gut RORgammat(+) ILC3 subsets that maintain mucosal barrier homeostasis. PMID: 24419270
    40. Enforced Gata3 expression is sufficient to enhance Th2 and group 2 innate lymphoid cell activity, and leads to increased susceptibility to allergic airway inflammation after exposure to inhaled harmless antigens that otherwise induce antigen tolerance. PMID: 24415780
    41. Results show the essential role of Gata3 in specifying the prosensory domain in the cochlea and in regulating the survival of spiral ganglion neurons, thus identifying a molecular mechanism underlying human HDR syndrome. PMID: 23666531
    42. IL-33 or a combination of IL-2 and IL-25 induced GATA3 phosphorylation. Both p38 and GATA3 are critical for the proliferation and productionon of IL-5 and IL-13. GATA3 plays a pivotal role in the effector functions of mature natural helper cells. PMID: 23851685
    43. Gata3 is independently required for the development of a pyloric outer longitudinal muscle fascicle, which is required for pyloric sphincter morphogenesis in mice. PMID: 24120474
    44. Gata3 inactivation in Pten-deficient prostates accelerates tumor invasion. PMID: 23428429
    45. a core regulatory subcircuit composed of Pax2/8, Gata3 and Lim1 turns on a deeper layer of transcriptional regulators while activating effector genes responsible for cell signaling and tissue organization. PMID: 23920117
    46. GATA-3-deficient T lymphocytes have defective responses to viral infection and alloantigen. PMID: 23708251
    47. GATA-3 regulates the self-renewal of long-term hematopoietic stem cells. PMID: 23974957
    48. results presented indicate that the jal mutation is a mutant allele of the Gata3 gene on mouse Chromosome 2 PMID: 23659281
    49. These results identify Gata3 as a critical early regulator of group 2 lymphoid cell development, thereby extending the paradigm of Gata3-dependent control of type 2 immunity to include both innate and adaptive lymphocytes. PMID: 23733962
    50. Macelignan attenuates the severity of murine experimental asthma by suppressing IL-4 producing CD4+Th cells through inhibition of GATA-3 activation. PMID: 23643632

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    T-cell specific.
  • 数据库链接：

    KEGG: mmu:14462

    STRING: 10090.ENSMUSP00000100041

    UniGene: Mm.313866