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  4. Recombinant Mouse Transcription factor 12 (Tcf12)

Recombinant Mouse Transcription factor 12 (Tcf12)

  • 中文名称：
    小鼠Tcf12重组蛋白
  • 货号：
    CSB-YP023293MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tcf12重组蛋白
  • 货号：
    CSB-EP023293MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tcf12重组蛋白
  • 货号：
    CSB-EP023293MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tcf12重组蛋白
  • 货号：
    CSB-BP023293MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tcf12重组蛋白
  • 货号：
    CSB-MP023293MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tcf12; Alf1; MebTranscription factor 12; TCF-12; Class A helix-loop-helix transcription factor ME1; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-706
  • 氨基酸序列
    MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV EQQLHEHLQD AMSFLKDVCE QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP TSHSDIHSLL GPSHNASIGN LNSNYGGSSL VTNSRSASMV GTHREDSVSL NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT EIKTENKEKD ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV ILSLEQQVRE RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST NPMGHL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
  • 基因功能参考文献：
    1. Study results place HEB at the top of the known gene network that regulates gammadeltaT17 cell programming. PMID: 29222418
    2. Tcf12 is a transcription factor highly expressed in the nuclei of stem cells and its downregulation plays an essential role in osteoblast differentiation. PMID: 27574032
    3. we identified transcription factor TCF12 as a new target of miR-211 in oral squamous cell carcinoma PMID: 27221705
    4. HEB is a fundamental link between Nodal signalling, the derepression of a specific class of poised promoters during differentiation, and lineage specification in mouse embryonic stem cells PMID: 25775035
    5. severe bilateral coronal synostosis occurs in mice with 50% of the wild-type dosage of both the Tcf12 and Twist1 genes highlights the key role of TCF12 acting with TWIST1 in the normal development of the coronal sutures PMID: 23354436
    6. Deficiency in the E proteins, E2A and HEB, led to increased frequency of terminally differentiated effector KLRG1(hi) CD8(+) T cells in mice during infection, and decreased generation of longer-lived memory-precursor cells during the immune response. PMID: 22585759
    7. Deletion of HEB and E2A in DP thymocytes specifically blocked the development of CD4(+) lineage T cells. Furthermore, deletion of the E protein inhibitors Id2 and Id3 allowed CD4(+) T cell development but blocked CD8(+) lineage development. PMID: 22425249
    8. the earliest event in B-cell specification involves the induction of FOXO1 expression and requires the combined activities of E2A and HEB PMID: 21972416
    9. These results showed a new set of interactions between HEB, Notch1, and GATA3 that regulate the T-cell fate choice in developing thymocytes. PMID: 21189289
    10. HEBAlt enforces T-lineage cell development during hematopoiesis.One role of HEBAlt may be to suppress myeloid development in developing T-cell precursors. PMID: 21115673
    11. Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt. PMID: 21061441
    12. HEBAlt can suppress B cell or myeloid potential in a context-specific manner, which suggests a role for this factor in maintaining T lineage priming prior to commitment. PMID: 20826759
    13. HEB is a specific and essential factor in T-cell development and in the generation of the iNKT cell lineage. PMID: 20154672
    14. the dosage of HEB determines pT alpha gene expression in immature T cells PMID: 12566462
    15. the Tcf12 gene may be involved in the control of proliferating neural stem cells and progenitor cells PMID: 15018808
    16. repression of E47/HEB by tal1/scl induces leukemia PMID: 15193261
    17. dHAND/E-protein (E2A, ME2, and ALF1) heterodimers have distinct DNA binding specificities PMID: 15351717
    18. A new transcription factor HEBAlt is specifically required for the efficient generation of early T lymphocyte precursors. PMID: 16785505
    19. Data show that upon induction of differentiation, a MyoD-HEB beta complex binds to the E1 E-box of the myogenin promoter, leading to transcriptional activation. PMID: 16847330
    20. ME1 mRNA is first detected in the jejunum and ileum two weeks after birth when the number of Paneth cells starts to increase. PMID: 17405739
    21. These results suggest that E2A and HEB are not only critical for T cell differentiation but also necessary to retain developing thymocytes in cell cycle arrest before pre-TCR expression. PMID: 17442955
    22. HEB and E2A as the gatekeepers that maintain cells at the DP stage of development until a functional alphabetaTCR is produced. PMID: 18093538
    23. The expression of E2-2, E2A, HEB as well as Id mRNA during T cell development, was analyzed. PMID: 18384878
    24. key residues in AML1-ETO for HEB association were mutated and these mutations do not impair the ability of AML1-ETO to enhance the clonogenic capacity of primary mouse bone marrow cells PMID: 19204326

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:21406

    STRING: 10090.ENSMUSP00000034755

    UniGene: Mm.171615