Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Transcription factor 4 (Tcf4)

Recombinant Mouse Transcription factor 4 (Tcf4)

  • 货号：
    CSB-YP023302MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023302MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023302MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023302MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023302MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tcf4
  • Uniprot No.：
  • 别名：
    Tcf4; Itf2; Sef2; Transcription factor 4; TCF-4; Class A helix-loop-helix transcription factor ME2; Immunoglobulin transcription factor 2; ITF-2; MITF-2; SL3-3 enhancer factor 2; SEF-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-670
  • 氨基酸序列
    MHHQQRMAAL GTDKELSDLL DFSAMFSPPV SSGKNGPTSL ASGHFTGSNV EDRSSSGSWG TGGHPSPSRN YGDGTPYDHM TSRDLGSHDN LSPPFVNSRI QSKTERGSYS SYGRENVQGC HQQSLLGGDM DMGNPGTLSP TKPGSQYYQY SSNNARRRPL HSSAMEVQTK KVRKVPPGLP SSVYAPSAST ADYNRDSPGY PSSKPAASTF PSSFFMQDGH HSSDPWSSSS GMNQPGYGGM LGNSSHIPQS SSYCSLHPHE RLSYPSHSSA DINSSLPPMS TFHRSGTNHY STSSCTPPAN GTDSIMANRG TGAAGSSQTG DALGKALASI YSPDHTNNSF SSNPSTPVGS PPSLSAGTAV WSRNGGQASS SPNYEGPLHS LQSRIEDRLE RLDDAIHVLR NHAVGPSTAV PGGHGDMHGI MGPSHNGAMG SLGSGYGTSL LSANRHSLMV GAHREDGVAL RGSHSLLPNQ VPVPQLPVQS ATSPDLNPPQ DPYRGMPPGL QGQSVSSGSS EIKSDDEGDE NLQDTKSSED KKLDDDKKDI KSITRSRSSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDAANHMGQM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.
  • 基因功能参考文献：
    1. The findings reveal the commitment and flexibility of E2-2high progenitor differentiation and imply that pertinent tuning machinery is present in the gut microenvironment. PMID: 29106601
    2. TCF4 is broadly expressed in cortical and subcortical structures in the developing and adult mouse brain. Tcf4 haploinsufficiency in mice replicated structural brain anomalies observed in Pitt-Hopkins syndrome patients. PMID: 29588831
    3. Our findings indicate that H2O2 inhibits NaV1.5 expression by activating the Wnt/b-catenin signaling and beta-catenin interacts with TCF4 to transcriptionally suppress cardiac NaV1.5 expression. PMID: 27068063
    4. Hdac2 isoform-selective knockdown was sufficient to rescue memory deficits in Tcf4(+/-) mice. PMID: 27568567
    5. We report that TCF4 comprises two transcriptional isoforms, both of which are required for optimal plasmacytoid DC development in vitro PMID: 27986456
    6. these data identified E2A and E2-2 as central regulators of B cell immunity. PMID: 27261530
    7. down-regulation of Id3 in B cells is essential for releasing E2A and E2-2, which in a redundant manner are required for antigen-induced B cell differentiation. PMID: 27217539
    8. TCF-4 as a co-activator of p65 in the potentiation of proinflammatory cytokine production in macrophages and aggravation of high-fat diet induced chronic inflammation and insulin resistance in mice. PMID: 27129186
    9. Data indicate that upregulation of E2-2 protein markedly attenuated the inhibitor of DNA-binding 1 (ID1)-mediated increase in endothelial progenitor cells (EPCs) proliferation and migration. PMID: 27635429
    10. This study demonstrated that Tcf4tg mice displayed significantly delayed conditioning when pre-exposed to the conditioned stimulus (12 kHz) but not when pre-exposed to a different stimulus PMID: 26404636
    11. We conclude that E2-2 inhibited EPC proliferation via suppressing their autophagy, and E2-2 regulated EPC autophagy by mediating the expression of ATG7. PMID: 26986900
    12. demonstrated that Id1 and E2-2 are critical regulators of EPCs function in vitro. Id1 interacts with E2-2 and relieves the E2-2-mediated repression of FGFR1 and VEGFR2 expression to modulate EPCs functions PMID: 26476925
    13. TCF4 is a regulator of neuronal intrinsic excitability in part by repression of Kcnq1 and Scn10a. PMID: 26971948
    14. A disease-promoting role of E2-2 dependent pDCs in the pancreas. PMID: 26624013
    15. Genetic disruption of Itf2 on the Apc(Min/+) background results in earlier death and a significant increase in tumor number and size in the small intestine. PMID: 25869068
    16. Results show that binding of the transcription factor Tcf4 correlates with hypo-methylation and demonstrate that Tcf4 is one of the factors contributing to formation of differentially methylated regions. PMID: 23714178
    17. Wnt/beta-catenin stimulation may repress BACE1 transcription via binding of TCF4 to BACE1 gene, and therefore, activation of the Wnt pathway may hold the key to new treatments of Alzheimer disease PMID: 25384422
    18. study suggests that CREPT acts as an activator to promote transcriptional activity of the beta-catenin.TCF4 complex in response to Wnt signaling. PMID: 24982424
    19. Dendritic cell-specific Tcf4 haplodeficiency ameliorated systemic lupus erythematosus-like disease caused by the overexpression of the endosomal RNA sensor Tlr7. PMID: 25180061
    20. beta-catenin patterns zonal liver metabolism together with Tcf-4, Hnf-4alpha, and xenobiotic nuclear receptors. PMID: 24214913
    21. Zac1 interacts with two cis-regulatory elements in the Tcf4 gene locus. PMID: 24396065
    22. The data of this study indicated that the reduced cognitive performance in Tcf4tg mice includes deficits at the level of attention and behavioral anticipation. PMID: 23069005
    23. increased beta-catenin/Tcf4 levels i) correlated with reduced TRalpha1 transcriptional activity on its target genes and, ii) were likely responsible for the shift of TRalpha1 binding on WNT targets PMID: 22509275
    24. We show that forced expression of Tcf4 or a stabilised beta-catenin mutant is sufficient to promote macrophage differentiation in response to GM-CSF and that GM-CSF can regulate beta-catenin stability, most likely via GSK3b. PMID: 22099176
    25. miR-155 directly suppresses the expression of the transcription factor TCF4. PMID: 21460854
    26. continuous expression maintains the cell fate of mature plasmacytoid dendritic cells PMID: 21145760
    27. published beta-catenin coactivators p300 and BRG1 displayed a more pleiotropic target gene expression profile controlling Wnt and other pathways. tcf4, mllt10/af10, and dot1l are co-expressed in Wnt-driven tissues in zebrafish PMID: 21103407
    28. Unlike other intrinsic regulators of fiber type, low levels of Tcf4 in myogenic cells promote both slow and fast myogenesis, thereby promoting overall maturation of muscle fiber type. PMID: 21177349
    29. These observations provide insight for the first time into the physiological function of TCF4 in the adult brain and its possible contributions to neuropsychiatric disease condition. PMID: 20434134
    30. analysis of control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines PMID: 19924301
    31. KLF4 directly interacts with TCF4 independently of beta-catenin and that KLF4 and TCF4 are expressed in similar patterns within the large intestine PMID: 19901072
    32. E2-2 is required for optimal expansion of pre-B cells, E2-2 modulates the follicular vs marginal zone B-cell populations. PMID: 17082585
    33. VMD2 is regulated by the MITF-TFE family through two E-boxes, with E-box 1 required for a direct interaction of MITF-TFE factors and E-box 2 for binding of the as yet unidentified factors PMID: 17085443
    34. Tcf4 interacts with Math1 to regulate differentiation of a specific subset of neuronal progenitors PMID: 17878293
    35. The bHLH transcription factors, splice variants, ITF-2A and ITF-2B, were identified as the Id1 and Id2 interacting protein during pregnancy. PMID: 18519032
    36. SMRT and NCoR have important roles in the regulation of beta-catenin-TCF4-mediated gene transcription PMID: 18632669
    37. These results identify E2-2 as a specific transcriptional regulator of the plasmacytoid dendritic cells lineage in mice and humans and reveal a key function of E proteins in the innate immune system. PMID: 18854153
    38. The involvement of AR, beta-catenin, and TCF-4 pathway during androgen action to activate a number of Wnt target genes, including Fst, and cross communication with the Smad signaling pathway during myogenesis. PMID: 18948405
    39. Data show that GFP directly reports Tcf4 activation in vivo and tracks the predicted increases in Tcf4 activation that result from Apc inactivation. PMID: 19263440
    40. The identification of class I basic Helix-Loop-Helix factor E2-2 (TCF4/ITF2) as a new epithelial-mesenchymal transition regulator is reported. PMID: 19295128
    41. Studies present the precise distribution of gene expression of canonical Wnt pathway transcriptional regulators, Tcf1, Lef1, Tcf3, Tcf4 and beta-catenin between PMID: 19303461
    42. embryonic progenitor cells and postnatal hair follicle stem cells coexpress Tcf3 and Tcf4, which can act as transcriptional activators or repressors. PMID: 19718027

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in the cerebral cortex, Purkinje and granule cell layers of the cerebellum, olfactory neuroepithelium, pyramidal cells of hippocampal layers CA1-CA4, and in the granular cells of the dentate gyrus.
  • 数据库链接：

    KEGG: mmu:21413

    STRING: 10090.ENSMUSP00000077577

    UniGene: Mm.4269