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  4. Recombinant Mouse Transcription factor 7 (Tcf7)

Recombinant Mouse Transcription factor 7 (Tcf7)

  • 中文名称：
    小鼠Tcf7重组蛋白
  • 货号：
    CSB-YP023305MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tcf7重组蛋白
  • 货号：
    CSB-EP023305MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tcf7重组蛋白
  • 货号：
    CSB-EP023305MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tcf7重组蛋白
  • 货号：
    CSB-BP023305MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tcf7重组蛋白
  • 货号：
    CSB-MP023305MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tcf7; Tcf-1; Tcf1Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-419
  • 氨基酸序列
    MPQLDSGGGG AGRGDDLGAP DELLAFQDEG EEQDDKNRDS PVGPERDLAE LKSSLVNESE GAAAGAGVPG PGVRVHGEAE GAPEALGREH TSQRLFPDKL PESLEDGLKA PECTSGMYKE TVYSAFNLLM PYPPASGAGQ HPQPQPPLHN KPGQPPHGVP QLSPLYEHFS SPHPTPAPAD ISQKQGVHRP LQTPDLSGFY SLTSGSMGQL PHTVSWPSPP LYPLSPSCGY RQHFPAPTAA PGAPYPRFTH PSLMLGSGVP GHPAAIPHPA IVPSSGKQEL QPYDRNLKTQ AEPKAEKEAK KPVIKKPLNA FMLYMKEMRA KVIAECTLKE SAAINQILGR RWHALSREEQ AKYYELARKE RQLHMQLYPG WSARDNYGKK KRRSREKHQE STTGGKRNAF GTYPEKAAAP APFLPMTVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages. Inhibits the developmental program of IL17A effector gamma-delta T-cell subsets via regulating the transcription of T-cell lineage effector proteins. Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.
  • 基因功能参考文献：
    1. Naive CD8+ T Cell TCF7 expression is FOXO1 independent. PMID: 28973925
    2. the inhibition of beta-catenin's TCF-dependent transcriptional activity, independent of its protein expression level, retains the naive ground state pluripotency in mouse embryonic stem cells. PMID: 28577307
    3. The balance between CD4(+) cytotoxic T cell and follicular helper T(Tfh) differentiation heavily depends on the class of infecting virus and is jointly regulated by the Tfh-related transcription factors Bcl6 and Tcf7 (encoding TCF-1) and by the expression of the inhibitory receptors PD-1 and LAG3. PMID: 27806296
    4. data reveal that T cell factor 1 (Tcf1) long and short isoforms have distinct, yet complementary, functions and may represent an evolutionarily conserved means to ensure proper programming of CD8(+) and CD4(+) T cell responses to viral infection PMID: 28652395
    5. Tcf7 levels are lower in islets taken from diabetic mice. Knockdown of TCF7 in islets impairs the cytoprotective responsiveness to GIP and enhances the magnitude of apoptotic injury. PMID: 26642437
    6. TCF-1-deficient CD4+ CD8+ double positive thymocytes fail to undergo TCR alpha Valpha14-Jalpha18 rearrangement and produce significantly fewer Natural killer T cells. PMID: 25536344
    7. The data suggest that miR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells. PMID: 26006243
    8. this study proposes a regulatory role for TCF7 in limiting access to the Treg lineage PMID: 26324778
    9. Our results support a critical role for miR-22-3p and its target, Tcf7, in the pathogenesis of diabetes by upregulating gluconeogenesis. PMID: 26193896
    10. TCF-1 was essential for both the initiation of T(FH) differentiation and the effector function of differentiated T(FH) cells during acute viral infection. TCF-1 promoted Bcl-6 expression but repressed Blimp1 expression. PMID: 26214740
    11. Chromatin immunoprecipitation sequencing revealed the transcription factor Tcf7 and the chemokine receptor Ccr7 as Foxo1-bound target genes, which have critical functions in central-memory T cell differentiation and trafficking. PMID: 23932570
    12. Our data support that the loss of Tcf7 diminishes hematopoietic stem cell function. PMID: 23212152
    13. TCF-1 mediates repression of Notch pathway in T lineage-committed early thymocytes. PMID: 23660861
    14. These results are reminiscent of the critical roles of TCF-1 in early T cell development. PMID: 23601684
    15. The repression of syndecan-2 by Wnt/beta-catenin/TCF signaling contributes to the resistance of osteosarcoma cells to doxorubicin. PMID: 22550000
    16. TCF-1-deficient (Tcf7(-/-)) mice developed aggressive T cell malignancy. PMID: 23103132
    17. TCF-1 and lymphoid enhancer-binding factor (LEF)-1 are essential for generation of memory precursors and functional memory CD8+ T cells. PMID: 22875805
    18. Together with RUNX1, TCF7 regulates a network of transcription factors that characterize the CD34+ cell state. PMID: 22412390
    19. TCF1 and beta-catenin regulate the generation of PLZF-expressing thymocytes and thereby facilitate the generation of memory-like CD8 T cells in the thymus. PMID: 22492686
    20. The TCF-1-mediated repression of IL-17 is critical for peripheral T cells to generate balanced immune responses. PMID: 21935461
    21. Tcf-1 is shown to be a gatekeeper of T-cell fate and Tcf-1 is directly activated by Notch signals. PMID: 22109558
    22. our data demonstrated that TCF-1 enhances CD4(+)CD8(+) double-positive thymocyte survival through transcriptional upregulation of RORgammat PMID: 22039299
    23. showed that TCF-1 deficiency limited proliferation of CD8(+) effector T cells and impaired their differentiation toward a central memory phenotype PMID: 20727791
    24. show that mice lacking T cell factor 1 (Tcf-1), a nuclear effector of the canonical Wingless/Integration 1 (Wnt) signaling pathway, mount normal effector and effector memory CD8 T cell responses to infection with lymphocytic choriomeningitis virus. PMID: 20457902
    25. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. PMID: 12731066
    26. A role of the constitutive activation of beta-catenin/Tcf signaling in skin carcinogenesis is discussed. PMID: 15765534
    27. cyclooxygenase-2/PGE2 may exert pro-oncogenic actions through stimulating the beta-catenin/T cell factor-mediated transcription, which plays critical roles in colorectal carcinogenesis PMID: 15899904
    28. TCF/beta-catenin pathway enhances CD4 expression in vivo by recruiting TCF-1 to stimulate CD4 enhancer activity. PMID: 16585583
    29. vascular endothelial growth factor expression is induced through the glucocorticoid receptor-related phosphatidylinositol 3-kinase/Akt and beta-catenin/T-cell factor-dependent pathway in human endothelial cells PMID: 17008323
    30. pro-osteogenic action of beta-catenin requires interaction with BMP signaling, but not Tcf/Lef transcriptional activity PMID: 18247340
    31. Data demonstrate marked changes in gene expression after Wnt3a stimulus, and show that NIH3T3 fibroblasts expressing a fusion protein of beta-catenin and TCF7 resulted in the identification of 92 genes as putative TCF targets. PMID: 19563800
    32. TCF-1 initiates T(H)2 differentiation of activated CD4(+) T cells by promoting GATA-3 expression and suppressing IFN-gamma expression. PMID: 19648923

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  • 相关疾病：
    Defects in Tcf7 may allow the formation of epithelial tumors.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TCF/LEF family
  • 组织特异性：
    T-cell specific. Expressed in triple negative 2 subpopulations of T-cells and both the gamma-delta and alpha-beta T-cell lineages. Expressed in Il7 receptor positive innate-like T-cells in the mesenteric lymph nodes and spleen (at protein level).
  • 数据库链接：

    KEGG: mmu:21414

    STRING: 10090.ENSMUSP00000084055

    UniGene: Mm.31630