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  4. Recombinant Mouse Transcription factor E2F1 (E2f1)

Recombinant Mouse Transcription factor E2F1 (E2f1)

  • 货号：
    CSB-YP733786MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP733786MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP733786MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP733786MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP733786MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E2f1; Transcription factor E2F1; E2F-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-430
  • 氨基酸序列
    MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Positively regulates transcription of RRP1B.
  • 基因功能参考文献：
    1. E2f1 mediates high glucose-induced neuronal death in cultured mouse retinal explants. PMID: 28825879
    2. p63alpha protein up-regulates heat shock protein 70 expression via E2F1 transcription factor 1, promoting Wasf3/Wave3/MMP9 signaling and bladder cancer invasion PMID: 28794159
    3. The evidence has been presented that the retinoblastoma protein utilizes a cell-cycle-independent interaction with E2F1 to recruit EZH2 to diverse repeat sequences. PMID: 27889452
    4. germ-line loss of E2f1 or E2f3b, but not E2f3a, protected mice against hepatocellular carcinoma PMID: 28134624
    5. E2F1 hinders skin wound healing by suppressing VEGF expression, neovascularization, and macrophage recruitment. Strategies that target E2F1 may enhance wound healing. PMID: 27490344
    6. TERT has a role in neointima formation through epigenetic regulation of proliferative E2F1 target gene expression in smooth muscle cells. PMID: 27932351
    7. Expression of Kv10.1 driven by phosphorylated Rb/E2F1 contributes to G2/M progression of cancer and non-transformed cells. PMID: 27029528
    8. Spinal cord injury-induced activation of E2F1-2 mediates cell cycle activation, contributing to gliopathy and neuronal/tissue loss associated with motor impairments and post-traumatic hyperesthesia. PMID: 26505089
    9. In the initial stage of myocyte differentiation, transcription of the YB-1 gene was regulated by E2F1 and Sp1, and was then gradually replaced under the control of both MyoD and myogenin. PMID: 26279143
    10. Protease Omi facilitates neurite outgrowth by cleaving the transcription factor E2F1 in differentiated neuroblastoma cells; E2F1 is a substrate of Omi. PMID: 26238290
    11. accumulating E2f1 progressively recruits a Pontin/Reptin complex to open the chromatin conformation at E2f target genes and amplifies the E2f transcriptional response. PMID: 26639898
    12. A role for E2F1 and E2F2 as suppressors of replicative stress in differentiating cells, and the existence of a robust E2F-p53 regulatory axis in tissue homeostasis enabling and tumorigenesis preventing is shown. PMID: 25656653
    13. E2F1 promotes apoptosis of spermatogonia during the first wave of spermatogenesis, is involved in the maintenance of the spermatogonial stem cell pool in the adult and inhibits apoptosis of the meiotic germ cells. PMID: 26311345
    14. Data (including data from studies in knockout/transgenic mice) suggest CDKN1B-RB1-E2F1 (cyclin-dependent kinase inhibitor 1B-retinoblastoma 1-E2F1) pathway is essential for SSC (spermatogonial stem cell) self-renewal/protection against genomic damage. PMID: 25959802
    15. Conditional knockout of Poh1 alleles results in reduced E2F1 expression in primary mouse liver cells. PMID: 26510456
    16. E2F1 negatively regulates both Ogt and Mgea5 expression in an Rb1 protein-dependent manner. PMID: 26527687
    17. Interplay between E2F1, miR421 and Pink1 regulates mitochondrial morphology and cardiomyocyte death. E2F1 activates miR421 expression. E2F1 knockdown suppresses mitochondrial fragmentation, apoptosis and myocardial infarction by reducing miR421 levels. PMID: 26184432
    18. study identifies a novel signaling pathway composed of E2F1, miR-30b and CypD that regulates myocardial necrosis. PMID: 25301066
    19. limits cardiac neovascularization and functional recovery after myocardial infarction by suppressing VEGF and PlGF up-regulation through p53-dependent and -independent mechanisms, respectively PMID: 25341896
    20. Rb-deficient cells hijack and redeploy Myc and E2f3 from an S-G2 program essential for normal cell cycles to a G1-S program that re-engages ectopic cell cycles, exposing an unanticipated addiction of Rb-null cells on Myc. PMID: 26192440
    21. Kbtbd5 regulates skeletal muscle myogenesis through the regulation of E2F1-DP1 activity PMID: 25940086
    22. The release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis. PMID: 25257171
    23. Tudor-SN is a potential substrate of G1/S phase Cyclin-Dependent Kinases, and promotes cell cycle progression by facilitating E2F-1-mediated gene transcription. PMID: 25627688
    24. oncogenic activities of E2F1 and E2F3 in ErbB2- or Myc-triggered mammary tumorigenesis, and a tumor suppressor role of E2F2 in Myc-mediated mammary tumorigenesis PMID: 24276244
    25. E2F1 regulates cellular senescence by inhibiting FOXO3. PMID: 25344604
    26. ERBB2-dependent maintenance of E2F-1 expression is an important molecular conduit required to sustain a genetic program necessary for adult cardiovascular homeostasis and function and for the adaptive response of the heart to stress. PMID: 25246633
    27. Our present study reveals a novel autophagic regulating model that is composed of E2F1, miR-325 and ARC. Modulation of their levels may provide a new approach for tackling cardiac failure. PMID: 24531537
    28. showed that the D326V missense pRb bound to E2F1 but failed to interact with E2F2/3 PMID: 25088905
    29. E2F1 activated early growth response (Egr)-1 expression in a biphasic fashion as described in both human and mouse hepatocytes. PMID: 24619556
    30. MiR-383 promoted the expression of miR-320 which regulated granulosa cell functions by targeting E2F1 and SF-1 proteins. PMID: 24828505
    31. In Rb1-deficient pituitary tumorigenesis, Skp2 deletion or p27T187A mutation converts E2F1's role from proliferative to apoptotic. PMID: 24632684
    32. E2F1 promotes DNA repiar and suppresses tumor development. PMID: 24741006
    33. Our study demonstrates that E2F1 contributes to physiologic brain structure and function PMID: 24460902
    34. Data indicate that cultured Sfpi1(BN/BN) cells expressed elevated messenger RNA transcript and protein levels of E2F1, an important regulator of cell cycle entry. PMID: 24316397
    35. AMPKalpha2 significantly contributed to stabilization and activation of E2F1 by doxorubicin, forming a positive signal amplification loop. PMID: 24398673
    36. these data identify E2F1 as a key transcription factor modulating the expression of chromatin components in oligodendrocyte progenitor cells during the transition from proliferation to differentiation. PMID: 24453336
    37. Ei24 is a novel E2F1 target gene contributing to the survival of p53-deficient cells upon UVC irradiation and thus may have a potential significance as a therapeutic target of certain chemotherapy for treating p53-deficient tumors. PMID: 24014029
    38. These results suggest a model whereby Rb-E2f interactions are sufficient to maintain gene repression irrespective of LXCXE-dependent chromatin remodeling. PMID: 23574720
    39. Rb/E2F1 axis exerts essential functions not only in maintaining epidermal homoeostasis, but also in suppressing tumour development in epidermis PMID: 22890321
    40. pRb-E2F1 is an obesity suppression mechanism in arcuate nucleus POMC neurons and high fat diet-AMPK inhibits this mechanism by phosphorylating pRb in this location. PMID: 23403926
    41. This study provides evidence that the histone modification and E2F1 binding are integral parts of the mechanism for Lhx2 gene expression. PMID: 23036195
    42. These findings suggest that Brd2 is required for cell cycle exit and neuronal differentiation of neuroepithelial cells through the E2F1 pathway during mouse CNS development. PMID: 22885183
    43. The results identify E2F8 as a repressor and E2F1 as an activator of a transcriptional network controlling polyploidization in mammalian cells. PMID: 23064264
    44. E2f1-/-;mdx mice demonstrated a strong reduction of physiopathological signs of Duchenne muscular dystrophy. PMID: 22678059
    45. Cardiac hypertrophy can be induced in an E2F1-independent fashion and suggest that in contrast to previous reports, targeting E2F1 may not be a good therapeutic approach. PMID: 21738823
    46. Rb/E2F is required to coordinate the transition between proliferation and differentiation by controlling key aspects of differentiation through regulation of the Dlx1/Dlx2 bigene cluster. PMID: 22699903
    47. Data suggest that nuclear translocation of cardiac E2F1 is enhanced by high-fat diet (HFD) concomitant with induction of pyruvate dehydrogenase kinase 4 (PDK4); HFD may initiate early cardiac metabolic alterations through cyclin D1/E2F1/PDK4 axis. PMID: 22569253
    48. Transgenic-mtTFB1 mice exhibit enhanced 12S rRNA methylation in multiple tissues, increased E2F1 and apoptosis in the stria vascularis and spiral ganglion neurons of the inner ear, and progressive E2F1-dependent hearing loss. PMID: 22341444
    49. JAZ plays a dual role in cell cycle regulation by both repressing E2F1 transcriptional activity and activating p53 to facilitate efficient growth arrest in response to cellular stress PMID: 21715977
    50. E2F-1 and FoxO-1a belong to a complex transcriptional network that may modulate myocardial cell death during I/R injury. PMID: 21964190

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 数据库链接：

    KEGG: mmu:13555

    STRING: 10090.ENSMUSP00000099434

    UniGene: Mm.18036