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  4. Recombinant Mouse Transcription factor HES-7 (Hes7)

Recombinant Mouse Transcription factor HES-7 (Hes7)

  • 货号：
    CSB-YP806395MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806395MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806395MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806395MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806395MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hes7
  • Uniprot No.：
  • 别名：
    Hes7; Transcription factor HES-7; mHes7; Hairy and enhancer of split 7; bHLH factor Hes7
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-225
  • 氨基酸序列
    MVTRERAENR DGPKMLKPLV EKRRRDRINR SLEELRLLLL ERTRDQNLRN PKLEKAEILE FAVGYLRERS RVEPPGVPRS PGQDAEALAS CYLSGFRECL LRLAAFAHDA SPAARSQLFS ALHGYRRPKP PRPEAVDPGL PAPRPPLDPA SPILGPALHQ RPPVHQGPPS PRLAWSPSHC SSRAGDSGAP APLTGLLPPP PPPYRQDGAP KAPSLPPPAF WRPWP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.
  • 基因功能参考文献：
    1. Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis PMID: 29437832
    2. Lengthening the third Hes7 intron by 10 or 20 kb disrupts accurate RNA splicing and inactivates the gene. PMID: 22326607
    3. Fgf signaling was found to be a primary target for hypoxia, which causes phenotypic variations of heterozygous mutations in Hes7 or Mesp2, suggesting gene-environment interaction through this signaling. PMID: 23465881
    4. Hes7 is a key regulator of the pace of the segmentation clock. PMID: 23219549
    5. Tbx6 and the Wnt pathway cooperatively regulate proper Hes7 expression. PMID: 23326414
    6. introns lead to an approximately 19-min delay in the Hes7 gene expression, and mathematical modeling suggested that without such a delay, Hes7 oscillations would be abolished PMID: 21300886
    7. there are differential axial requirements for lunatic fringe and Hes7 transcription during mouse somitogenesis PMID: 19956724
    8. periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock PMID: 12783854
    9. instability of Hes7 is essential for sustained oscillation and for its function as a segmentation clock PMID: 15170214
    10. Lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis by forming negative feedback loop PMID: 16342160
    11. Hes7 oscillation is initiated by Fgf signaling and propagated/maintained anteriorly by Notch signaling. PMID: 17681139
    12. repressor functionality and the half-life of the protein is crucial for oscillatory expression of Hes7 and regular somite formation PMID: 17983837
    13. These results indicate that common amino acid residues are involved in both the transcriptional repressor activity and the instability of Hes7 protein, suggesting of a critical link between the transcription and degradation control. PMID: 18477475
    14. Sprouty4 does not oscillate in Hes7-null mutant mouse embryos, and Hes7 can inhibit FGF-induced transcriptional activity of the Sprouty4 promoter. PMID: 19440349

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:84653

    STRING: 10090.ENSMUSP00000024543

    UniGene: Mm.98505