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  4. Recombinant Mouse Transcription factor MafA (Mafa)

Recombinant Mouse Transcription factor MafA (Mafa)

  • 货号：
    CSB-YP806526MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806526MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806526MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806526MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806526MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mafa
  • Uniprot No.：
  • 别名：
    MafaTranscription factor MafA; Pancreatic beta-cell-specific transcriptional activator; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-359
  • 氨基酸序列
    MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL SSTPLSTPCS SVPSSPSFCA PSPGTGGGAG GGGSAAQAGG APGPPSGGPG TVGGASGKAV LEDLYWMSGY QHHLNPEALN LTPEDAVEAL IGSGHHGAHH GAHHPAAAAA YEAFRGQSFA GGGGADDMGA GHHHGAHHTA HHHHSAHHHH HHHHHHGGSG HHGGGAGHGG GGAGHHVRLE ERFSDDQLVS MSVRELNRQL RGFSKEEVIR LKQKRRTLKN RGYAQSCRFK RVQQRHILES EKCQLQSQVE QLKLEVGRLA KERDLYKEKY EKLAGRGGPG GAGGAGFPRE PSPAQAGPGA AKGAPDFFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.
  • 基因功能参考文献：
    1. Data suggest that hepatocytes can be reprogrammed into insulin-producing cells in vivo by transfection of neurogenin-3, Pdx1, and MafA genes using non-viral hydrodynamics injection; this procedure was used in treatment of streptozotocin diabetes; fasting blood glucose was reduced to normal. (Pdx1 = pancreatic and duodenal homeobox 1; MafA = v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A) PMID: 28100871
    2. Mafa was found to both potentiate the ability of Pdx1 to induce beta-cell formation from Ngn3-positive endocrine precursors and enable Pdx1 to produce beta-cells from alpha-cells. PMID: 28223284
    3. is necessary for the maintenance of normal insulin levels even in embryos. PMID: 25912440
    4. endogenous small-Maf factors negatively regulate beta-cell function by competing for MafA binding, and thus, the inhibition of small-Maf activity can improve beta-cell function PMID: 25763640
    5. Data, including data from studies using transgenic/knockout mice, confirm the expression of Mafa and Mafb is specific to insulin-secreting cells of the pancreas. PMID: 25273397
    6. These results suggest that MafA regulates the postnatal proliferation of beta-cells via prolactin signaling. PMID: 25126749
    7. results illustrate how consequential the reduction in Mafa activity is to islet beta-cell function under pathophysiological conditions. PMID: 25645923
    8. Results not only clearly define why MafA is an essential transcriptional regulator of islet beta-cells, but also why cell maturation involves coordinated actions with MafB. PMID: 24520122
    9. MAFA, MAFB, NKX6.1, and PDX1 activity provides a gauge of islet beta cell function, with loss of MAFA (and/or MAFB) representing an early indicator of beta cell inactivity PMID: 23863625
    10. under oxidative and nonoxidative conditions p38 MAPK directly binds to MafA and triggers MafA degradation via ubiquitin proteasomal pathway. PMID: 23660596
    11. In this study, we tried to establish an effective method of differentiation through the protein transduction of three transcription factors (Pdx1, NeuroD, and MafA) important to pancreatic beta cell development. PMID: 24292793
    12. We found that forcing MafA transgene expression, out of its normal developmental context, in Ngn3(+) endocrine progenitors blocked endocrine differentiation and prevented the formation of hormone(+) cells. PMID: 24183936
    13. We identified MAFA as a target that is downregulated by miR-204. The newly identified TXNIP-miR-204-MAFA-insulin pathway may contribute to diabetes progression. PMID: 23975026
    14. Findings suggest that Onecut1 suppresses MafA gene expression through the Foxa2-binding site. PMID: 23775071
    15. Characterization of an apparently novel beta-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. PMID: 23269676
    16. MicroRNA-30d induces insulin transcription factor MafA and insulin production by targeting mitogen-activated protein 4 kinase 4 (MAP4K4) in pancreatic beta-cells. PMID: 22733810
    17. Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
    18. Results indicate that Pdx1 and MafA drive DE progenitors towards insulin-producing cells. PMID: 22563503
    19. mechanism of transdifferentiation of pancreatic exocrine cells to beta (beta) cells in vivo by MafA PMID: 22150363
    20. Data show that when injected in utero into living mouse embryos, TAT-MafA significantly up-regulates target genes and induces enhanced insulin production as well as cytoarchitectural changes consistent with faster islet maturation. PMID: 21857924
    21. PA28gamma stimulates MAFA degradation through a novel molecular mechanism that is distinct from that for the degradation of p21 PMID: 21830322
    22. Compromised MafA expression contributes to beta-cell dysfunction in maturity onset diabetes of young mice. MafA mutant mice manifested symptoms associated with human type 2 diabetes. PMID: 21193557
    23. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    24. Data show that SUMOylation is a functional post-translational modification of MafA that negatively regulates its transcriptional and transforming activities. PMID: 20718938
    25. The bZIP transcription factor MafA is identified as the first specific marker of a subpopulation of "early c-ret" positive neurons characterized by medium-to-large diameters. PMID: 20213756
    26. Insight into the sequential manner by which MafA regulates islet beta-cell formation and maturation in mice. PMID: 20627934
    27. Polymorphisms of MafA associate with reduced expression of insulin in the thymus and susceptibility to type 1 diabetes in the NOD mouse as well as human type 1 diabetes. PMID: 20682694
    28. Correct spatial and inducible regulation of Mafa involves cross talk between trans-acting factors binding to multiple conserved cis elements located within distinct regions of the promoter. PMID: 20584984
    29. It is likely that the augmented expression of c-Jun in diabetic islets decreases MafA expression and thereby reduces insulin biosynthesis, which is often observed in type 2 diabetes. PMID: 20424231
    30. a novel relationship between the phosphoamino acid-rich transactivation and b-Zip domains in controlling MafA DNA-binding activity. PMID: 20208071
    31. Using conditional MafA knockout mice, it is demonstrated that MafA identifies the early ret-expressing sensory neurons in the dorsal root ganglia. PMID: 20213756
    32. Our results provide evidence that genetic interactions involving Ret and MafA progressively promote the differentiation and diversification of LTMs. PMID: 20064392
    33. MafA is a beta-cell-specific and glucose-regulated transcriptional activator for insulin gene expression and thus may be involved in the function and development of beta-cells as well as in the pathogenesis of diabetes PMID: 12368292
    34. These results strongly suggested that MafA was the beta-cell-enriched component of the RIPE3b1 activator PMID: 12917329
    35. mafA has a role in regulating insulin gene expression in the liver PMID: 15664997
    36. These results show that MafA is a key regulator of glucose-stimulated insulin secretion in vivo. PMID: 15923615
    37. FoxO1 protects against pancreatic beta cell failure through Neurod and Mafa induction. PMID: 16154098
    38. Results describe the chronological sequence of binding of MafA, E47/beta2 and PDX-1 to the insulin promoter in living beta-cells. PMID: 16412423
    39. MafB may have a dual role in regulating embryonic differentiation of both beta- and alpha-cells while MafA may regulate replication/survival and function of beta-cells after birth. PMID: 16580660
    40. Data show that FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site. PMID: 16847327
    41. MafA expression mediated by high glucose requires flux through the hexosamine biosynthetic pathway and the O-linked glycosylation of an unknown protein(s) by UDP-N-acetylglucosaminyl transferase PMID: 17142462
    42. Ripe3b1 induces islet cell differentiation in hepatic progenitor cells. PMID: 17239820
    43. Results suggest possible role of mafA in the pathophysiological states of impaired metabolic responses or inflammatory reactions. PMID: 17346669
    44. MafA has a critical role in islet beta-cell function PMID: 17636040
    45. Data show that MafA is constitutively phosphorylated by GSK3, and suggest a new glucose-sensing signaling pathway in islet beta cells that regulates insulin gene expression through the regulation of MafA protein stability. PMID: 17682063
    46. MafA-dependent activation of insulin II gene promoters was inhibited in the presence of Smad2/Smad4 or Smad3/Smad4 and a constitutively active TGF-beta type I receptor and this inhibition was dependent upon the presence of MafA. PMID: 17927952
    47. Significance of large Maf proteins to Pdx1 expression in beta cells, and in particular MafB during pancreatic development. PMID: 18522939
    48. mobility of MafA S65A was profoundly affected upon SDS-PAGE, with the S65E and S65D mutants influenced less due to their ability to serve as substrates for glycogen synthase kinase 3 PMID: 19004825
    49. study suggests that modification of MafA by SUMO modulates gene transcription and thereby beta cell function PMID: 19029092
    50. p38 MAPK is a major regulator of MafA protein stability under oxidative stress PMID: 19407223

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, Maf subfamily
  • 组织特异性：
    Expressed in brain, lung, spleen, pancreas and kidney. In the pancreas, expressed in the insulin-producing beta-cells of the islets of Langerhans (at protein level). Also expressed in the eye.
  • 数据库链接：

    KEGG: mmu:378435

    STRING: 10090.ENSMUSP00000054226

    UniGene: Mm.309589