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  4. Recombinant Mouse Transcription factor MafB (Mafb)

Recombinant Mouse Transcription factor MafB (Mafb)

  • 中文名称：
    小鼠Mafb重组蛋白
  • 货号：
    CSB-YP013315MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mafb重组蛋白
  • 货号：
    CSB-EP013315MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mafb重组蛋白
  • 货号：
    CSB-EP013315MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mafb重组蛋白
  • 货号：
    CSB-BP013315MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mafb重组蛋白
  • 货号：
    CSB-MP013315MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mafb
  • Uniprot No.：
  • 别名：
    Mafb; Krml; Maf1Transcription factor MafB; Maf-B; Kreisler; Segmentation protein Kr; Transcription factor Maf-1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-323
  • 氨基酸序列
    MAAELSMGQE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ PAGSVSSTPL STPCSSVPSS PSFSPTEPKT HLEDLYWMAS NYQQMNPEAL NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV THDDLGQHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHATAAATA AGGNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK LANSGFREAG STSDSPSSPE FFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain.
  • 基因功能参考文献：
    1. MafB appears to play an important role in secondary hyperparathyroidism by regulation of parathyroid hormone and cyclin D2 expression. PMID: 28964572
    2. The expression of the first protein in the classical complement pathway C1q is important for mediating efferocytosis and is reduced in Mafb-deficient macrophages. PMID: 29167450
    3. present findings indicate that MafB enhances efferocytosis by regulating Axl expression in RAW264.7 macrophages PMID: 29030012
    4. Therefore, MafB promotes differentiation in postmitotic keratinocytes and simultaneously has potential to promote growth when ectopically expressed in undifferentiated basal keratinocytes. PMID: 28418611
    5. MAFB localized in Leydig and Sertoli cells in testes at E18.5 while it localized in Leydig cells, Sertoli cells, and pachytene spermatocytes in adult testes. Examination revealed that MAFB-deficient testes developed normally by E18.5, and spermatogenesis was not disrupted in adult mice. MAFB MAFB might be critical for phagocytosis activity of Sertoli cells. PMID: 29324782
    6. MafB controls endothelial sprouting in vitro and in vivo in postnatal retinal angiogenesis. PMID: 28959057
    7. Macrophage-specific inhibition of MafB may destabilize atherosclerotic plaques in advanced lesions PMID: 27214395
    8. a protective role of oxLDL-associated LPS on beta-cell function during hyperlipidemia by inducing miR-155-5p, which prevents the upregulation of MafB and beta-to-alpha-cell reprogramming. PMID: 28970282
    9. Increased mafB at the common myeloid progenitor stage steers their lineage away from the megakaryocyte erythrocyte progenitor production and drives the terminal fate of these progenitors to form macrophages vs. dendritic cells, causing anemia, monocytosis, and dendritic cell loss. It is a transcriptional activator of M-CSFR and a repressor of transferrin receptors, promoting macrophages and plasmacytoid dendritic cells. PMID: 26992433
    10. An elevation of MSR1 levels in infiltrating myeloid cells observed 3 d after experimental stroke was dependent on the transcription factor Mafb. Mafb deficiency in infiltrating myeloid cells caused impaired clearance of DAMPs, more severe inflammation, and exacerbated neuronal injury in a murine model of ischemic stroke. PMID: 28394332
    11. a Mafb-driven Cre strain was generated to determine whether any dendritic cells (DCs) identified by Zbtb46-green fluorescent protein expression originate from a Mafb-expressing population. PMID: 27810926
    12. MafB deletion in maternal beta-cells also produced GDM, with inadequate beta-cell expansion accompanied by failure to induce PRLR-dependent target genes regulating beta-cell proliferation. These results unveil molecular roles for PRLR signaling in orchestrating the physiologic expansion of maternal beta-cells during pregnancy. PMID: 27217483
    13. MafB play an important role in promoting hindbrain choroid plexus development during embryogenesis PMID: 28025141
    14. Meanwhile, the observed reduction in the phagocytic activity of the MafB-shRNA-expressing cells was attenuated by ectopic expression of Fcgr3. Together, the results presented here indicate that MafB influences the phagocytic activity of macrophages by promoting Fcgr3, but not Fcgr2b, expression. PMID: 27847322
    15. Loss of MAFB Function Causes Duane Syndrome, Aberrant Extraocular Muscle Innervation, and Inner-Ear Defects. PMID: 27181683
    16. MafB and c-Maf have different expression patterns in macrophages, suggesting differences in function. PMID: 26996125
    17. MAFB is an important transcription factor involved in lymphangiogenesis. PMID: 26549461
    18. Study provides insights to elucidate mechanisms of gene regulation through androgen response elements present in Mafb 3'UTR for a better understanding of the processes of urethral masculinization. PMID: 26636186
    19. It contributes to the development of hyperglycemia in the Mafa-deficient background in adults as well as in embryos. PMID: 25912440
    20. MafB is only essential to islet alpha-cell activity and not beta-cell. PMID: 26554594
    21. findings represent the first evidence that the macrophage-affiliated MafB transcription factor participates in the acceleration of atherogenesis PMID: 24445679
    22. Data, including data from studies using transgenic/knockout mice, confirm the expression of Mafa and Mafb is specific to insulin-secreting cells of the pancreas. PMID: 25273397
    23. Mafb-deficient microglia cultured with GM-CSF barely extended their membrane protrusions, probably due to abnormal activation of RhoA, a key regulator of cytoskeletal remodeling. MafB is a negative regulator of GM-CSF signaling in microglia. PMID: 25998393
    24. high-resolution structures of the transcription factor MafB as a homodimer and as a heterodimer with c-Fos bound to variants of the Maf-recognition element PMID: 24530283
    25. Mafb is expressed in male genital tubercle (GT) mesenchyme. Exogenous androgen induces its mesenchymal expression in female GTs. AR signaling is necessary for its expression. Mafb KO male GTs have defective embryonic urethral formation. PMID: 25362053
    26. Mafb has a protective role in diabetic nephropathy through regulation of slit diaphragm proteins, antioxidative enzymes, and Notch pathways in podocytes. PMID: 24722438
    27. MAFA, MAFB, NKX6.1, and PDX1 activity provides a gauge of islet beta cell function, with loss of MAFA (and/or MAFB) representing an early indicator of beta cell inactivity PMID: 23863625
    28. Reduced number and morphofunctional change of alveolar macrophages in MafB gene-targeted mice. PMID: 24040127
    29. The efficiency of the MafB-induced multiple myeloma reprogramming of normal hematopoietic stem/progenitor cells to terminally differentiated malignant plasma cells is enhanced by p53 deficiency. PMID: 22983007
    30. the haematopoietic progenitor population can be the target for transformation in MafB-associated plasma cell neoplasias PMID: 22903061
    31. The data showed that Men1 inactivation led to MafB reexpression in mouse beta cells in vivo, and provides evidence that deregulated ectopic MafB expression may have a hitherto unknown role in adult beta cell proliferation and Men1-related tumorigenesis. PMID: 22120711
    32. The homozygous kreisler mutation eliminates most of rhombomere 5 and mis-specifies rhombomere 6, abolishes an early decrease in respiratory frequency within 10 min of hypoxia and an intrinsic hypoxic activation. PMID: 21839147
    33. High MafB following experimental burn plays an inhibitory role in monocyte-derived dendritic cell differentiation by regulating monocyte-colony factor receptor (M-CSFR) and GATA-1 expression. PMID: 21984745
    34. results suggest that MafB regulates later steps of parathyroid development, that is, separation from the thymus and migration toward the thyroid. MafB also regulates the expression of PTH in cooperation with Gcm2 PMID: 21713993
    35. Findings identify Mafb as one of the earliest direct targets of Cdx1 and show that Cdx1 plays a direct role in early hindbrain patterning. PMID: 21098558
    36. Insight into the sequential manner by which MafB regulates islet beta-cell formation and maturation in mice. PMID: 20627934
    37. MafB acts as a metastable switch tocontrol expression of IFN-beta. PMID: 20581830
    38. expressed in the inner root sheath layers in a lineage-restricted manner and are involved in hair morphogenesis PMID: 20060689
    39. Requirement for the downregulation of segmental kreisler expression prior to neuronal differentiation reflects the stage-specific roles of this gene PMID: 11880356
    40. Kreisler acts during the final stages of glomerular . The levels of Podocin, the gene mutated in NPHS2, and Nephrin, the gene mutated in NPHS, are slightly reduced in kr(enu)/kr(enu) podocytes. PMID: 12217315
    41. determination of dual function of Mafb in both segmentation and specification of anteroposterior identity in the hindbrain PMID: 12490204
    42. Kreisler regulates maintenance and expansion of rhombomere (r) 5 and specification of r6 but is not required for r3 development. PMID: 12701106
    43. Because MafA, MafB, and c-Maf were each capable of specifically binding to and activating insulin C1 element-mediated expression, our results suggest that all of these factors play a role in islet beta-cell function. PMID: 12917329
    44. In Mafb(-/-) mice the preBotzinger Complex, but not major neuromodulatory groups, presents severe anatomical defects with loss of cellularity, suggesting an essential role of MafB in central respiratory control PMID: 14513037
    45. Low-density lipoprotein receptor-related protein intracellular domain co-localizes with MafB to the nucleus and negatively regulates its transcriptional activity PMID: 15135046
    46. These data indicate that maf-B mRNA is expressed during development of the eyelid closure in mice. PMID: 15597194
    47. Initiation of Kreisler expression has direct requirement for vHNF1. PMID: 16274963
    48. The data also identify Gbx2, Dlx5, Wnt2b and Otx2 as key otic genes ultimately affected by perturbation of the kr/mafB-hindbrain pathway. PMID: 16325169
    49. Within the adult pancreas, MafB is only expressed in islet alpha-cells and contributes to cell type-specific expression of the glucagon gene through activation of a conserved control element found between nucleotides -77 to -51. PMID: 16443760
    50. MafB may have a dual role in regulating embryonic differentiation of both beta- and alpha-cells while MafA may regulate replication/survival and function of beta-cells after birth. PMID: 16580660

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, Maf subfamily
  • 组织特异性：
    Expressed in pancreatic alpha-cells (glucagon-positive cells), in podocytes of the kidney and macrophages (at protein level). Most abundant in kidney, gut, lung and brain.
  • 数据库链接：

    KEGG: mmu:16658

    STRING: 10090.ENSMUSP00000096728

    UniGene: Mm.330745