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  4. Recombinant Mouse Transcriptional enhancer factor TEF-1 (Tead1)

Recombinant Mouse Transcriptional enhancer factor TEF-1 (Tead1)

  • 中文名称：
    小鼠Tead1重组蛋白
  • 货号：
    CSB-YP023363MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tead1重组蛋白
  • 货号：
    CSB-EP023363MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tead1重组蛋白
  • 货号：
    CSB-EP023363MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tead1重组蛋白
  • 货号：
    CSB-BP023363MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tead1重组蛋白
  • 货号：
    CSB-MP023363MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tead1; Tcf13; Tef-1; Tef1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSRDFHSKLK DQTAKDKALQ HMAAMSSAQI VSATAIHNKL GLPGIPRPTF PGGPGFWPGM IQTGQPGSSQ DVKPFVQQAY PIQPAVTAPI PGFEPTSAPA PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV HIGHANHSYS DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD DAGAFYGVSS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV YRINRSPMCE YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL LCMACVFEVS NSEHGAQHHI YRLVKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
  • 基因功能参考文献：
    1. The YAP/TEAD1 complex binds to DNA element and regulates the expression of genes involved in cell growth..our data demonstrate an important role of TEAD1 in early development in mice, and the floxed TEAD1 mouse model will be a valuable genetic tool to determine the temporal and tissue-specific functions of TEAD1. PMID: 29193599
    2. Wnt/beta-catenin signaling via Axin2 is required for myogenesis and, together with YAP/Taz and Tead1, active in IIa/IIx muscle fibers PMID: 27578179
    3. Overexpression of TEAD1 induced Treg cell differentiation. TEAD sequesters TAZ and inhibits TH17 development. PMID: 28504697
    4. YAP and TEAD1, key downstream effectors of the Hippo pathway, are specifically expressed in Muller cells. We also uncovered a deregulation of the expression and activity of Hippo/YAP pathway components in reactive Muller cells under pathologic conditions. PMID: 28384715
    5. Data show that TEAD family of transcription factors Tead1 and Tead4-regulated gene expression in differentiating primary myoblasts. PMID: 28178271
    6. Cells with reduced Tead activity became losers, whereas cells with increased Tead activity became super-competitors. Tead directly regulated Myc RNA expression, and cells with increased Myc expression also became super-competitors. PMID: 25588835
    7. The PDZ-binding motif of YAP is critical for YAP-mediated oncogenesis, and that this effect is mediated by YAP's co-activation of TEAD-mediated CTGF transcription. PMID: 24380865
    8. TEAD1 regulates C2C12 differentiation through negatively regulating the expression of Ccne1, which can explain the transition between proliferation and differentiation. PMID: 23220227
    9. TEAD1 is shown to be a mediator of skeletal muscle development. PMID: 21211055
    10. increased TEAD-1 can induce characteristics of cardiac remodeling associated with cardiomyopathy and heart failure. PMID: 20194497
    11. These results are consistent with two plausible models of cryptic MCAT enhancer regulation by Pur alpha, Pur beta, and MSY1 involving either competitive single-stranded DNA binding or masking of MCAT-bound transcription enhancer factor-1. PMID: 11751932
    12. Transcription enhancer factor 1 binds multiple muscle MEF2 and A/T-rich elements during fast-to-slow skeletal muscle fiber type transitions PMID: 12861002
    13. VITO-1, a new scalloped interaction domain-containing protein, binds to TEF1 in vitro and strongly stimulates transcription of a reporter plasmid together with TEF-1 PMID: 14762206
    14. p38 MAPKs regulate TEF-1 and C/EBPbeta transcriptional activity in the absence of environmental stress. PMID: 16492136
    15. Sveinsson's chorioretinal atrophy pathogenesis may be due to a loss-of-function of TEAD1 affecting the regulation of its target genes. PMID: 17689488
    16. RTEF-1 bound to the element-containing region within the smooth muscle alpha-actin promoter in myofibroblasts, whereas TEF-1 was bound to the same region in differentiated smooth muscle cells PMID: 17823374
    17. Tead1 and Tead2 are functionally redundant, use YAP as a major coactivator, and support notochord maintenance as well as cell proliferation and survival in development. PMID: 18332127
    18. TEAD-1 has a role in modulating slow muscle gene expression PMID: 18978355
    19. The primary cellular origin of circumpapillary dysgenesis of the pigment epithelium is within the choroid instead of the pigment epithelium. PMID: 19410955
    20. A transcription cofactor required for skeletal muscle differentiation that modifies DNA target selectivity of the Transcriptional Enhancer Factor-1 (TEF-1) transcription factors. PMID: 15287000

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    In developing skeletal muscle and myocardium, in mitotic neuroblasts both in the brain and spinal cord. At later stages of embryogenesis expressed in several developing structures such as the olfactory system, the intestine, and the kidney.
  • 数据库链接：

    KEGG: mmu:21676

    UniGene: Mm.24685