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  4. Recombinant Mouse Transient receptor potential cation channel subfamily V member 5 (Trpv5)

Recombinant Mouse Transient receptor potential cation channel subfamily V member 5 (Trpv5)

  • 中文名称：
    小鼠Trpv5重组蛋白
  • 货号：
    CSB-YP025097MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Trpv5重组蛋白
  • 货号：
    CSB-EP025097MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Trpv5重组蛋白
  • 货号：
    CSB-EP025097MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Trpv5重组蛋白
  • 货号：
    CSB-BP025097MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Trpv5重组蛋白
  • 货号：
    CSB-MP025097MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Trpv5
  • Uniprot No.：
  • 别名：
    Trpv5; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC1; Osm-9-like TRP channel 3; OTRPC3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-723
  • 氨基酸序列
    MGAKTPWIQLQKLLNWWVRDQDWNQHVDQLHMLQQKSIWESPLLRAAKENDMCTLKKLQH DQNCDFRQRGALGETALHVAALYDNLDAAIMLMEAAPYLVTESTLCEPFVGQTALHIAVM NQNVNLVRALLARGASASARATGSAFHRSSHNLIYYGEHPLSFAACVGSEEIVRLLIEHG ADIRAQDSLGNTVLHILVLQPNKTFACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLA GVEGNTVMFQHLMQKRKRIQWSFGPLTSSLYDLTEIDSWGEELSFLELVVSSKKKEARQI LEQTPVKELVSLKWKKYGQPYFCLLGALYIFYMVCFTTCCVYRPLKFRDANRTHVRDNTI MEQKSLQEAYVTYQDKIRLVGELVTVIGAVIILLLEIPDIFRVGASRYFGQTVLGGPFHV IIITYASLVLLTMAMRLTNVNGEVVPMSMALVLGWCSVMYFARGFQMLGPFTIMIQKMIF GDLLRFCWLMAMVILGFASAFYIIFQTEDPDNLGEFSDYPTAMFSTFELFLTIIDGPANY RVDLPFMYSVTYATFAIIATLLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERK MPRFLWPRSGICGCEYGLGDRWFLRVEHHQEQNPYRVLRYVEAFKSSDKEEVQEQLSEKQ PSGTETGTLARGSVVLQTPPLSRTTSLSSNSHRGWEILRRNTLGHLNLGLDPGEGDGEEI YQF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
  • 基因功能参考文献：
    1. life-long challenge of Ca2+ homeostasis present in Trpv5-/- mice causes accelerated bone aging and a low cortical and trabecular bone mass phenotype PMID: 27102152
    2. beta-gal is present in the pro-urine from where it is thought to stimulate TRPV5 activity. PMID: 26747426
    3. A novel primary cell model with TRPV5-dependent Ca(2+) transport characteristics was successfully established. PMID: 24557712
    4. Phosphorylation of beta1-AR by PKA stimulates active Ca(2+)influx through TRPV5. PMID: 24828496
    5. The expression of TRPV5 channel is regulated by estrogen via estrogen receptor in osteoblasts. PMID: 24150765
    6. Uromodulin upregulates TRPV5 by decreasing caveolin-1 dependent endocytosis of TRPV5. PMID: 23466996
    7. In colitis in mice, tumor necrosis factor and interferon-gamma reduced expression of Klotho, which otherwise would protect TRPV5 from hypersialylation and cytokine-induced TRPV5 endocytosis, UBR4-dependent ubiquitination, degradation, and Ca(2+) wasting. PMID: 23747339
    8. The TRPV5 S682P mutant is a functionally significant factor in autosomal dominant hypercalciuria. PMID: 23383183
    9. promoter driven Cre recombinase expression will be useful for inducing DCT2 and CNT specific gene silencing of various channels, pumps, carriers, and receptors PMID: 21290301
    10. Calcitonin augments the renal reabsorptive capacity for Ca(2+). This increase is likely to occur independently of TRPV5. PMID: 19965988
    11. TrpV5 mediates basal renal calcium absorption in null mutant mice lacking calcium channel beta3 subunits (CaVbeta3) and multimeric calcium channel entry via distal convoluted renal tubules. PMID: 19767875
    12. found in stria vascularis, organ of Corti, vestibular sensory cells and dark cells, and less intensely in the spiral and vestibular ganglion cells, in both old and young mice PMID: 19922080
    13. the key function of TRPV5 is in active Ca2+ reabsorption and it has an essential role in Ca2+ homeostasis PMID: 14679186
    14. demonstration of the significance of SGK1 and NHERF2 as TRPV5 modulators which are likely to participate in the regulation of calcium homeostasis by 1,25(OH)2D3 PMID: 15665527
    15. Hypervitaminosis D is of crucial importance to maintain normocalcemia in impaired Ca(2+) reabsorption in TRPV5 knockout mice. PMID: 16148038
    16. TRPV5 is stimulated by klotho; klotho hydrolyzes extracellular sugar residues on TRPV5, entrapping the channel in the plasma membrane PMID: 16239475
    17. These data demonstrated the important role of Rab11a in the trafficking of TRPV5 and TRPV6. PMID: 16354700
    18. binding site for NHERF4 localized in a conserved region in the carboxyl terminus of TRPV5 and was distinct from the binding site of the PDZ protein NHERF2. NHERF4 predominantly localized at the plasma membrane of X. PMID: 16565876
    19. downregulation of TRPV5 and TRPV6 is likely involved in the impaired Ca2+ (re)absorption during aging PMID: 16705151
    20. One aspect of vestibular dysfunction in Pendred syndrome is a pathological elevation of endolymphatic [Ca(2+)] due to luminal acidification and consequent inhibition of TRPV5/6-mediated Ca(2+) absorption. PMID: 17200157
    21. Unraveling molecular mechanisms of TRPV5 channel regulation by antiaging hormone klotho, tissue kallikrein & extracellular pH demonstrated existence of novel regulatory mechanisms of active calcium reabsorption acting from tubular lumen[review] PMID: 17565273
    22. Klotho participates in specific removal of alpha2,6-linked sialic acids and regulates cell surface retention of TRPV5 through this activity PMID: 18606998

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV5 sub-subfamily
  • 组织特异性：
    Detected in kidney cortex (at protein level).
  • 数据库链接：

    KEGG: mmu:194352

    STRING: 10090.ENSMUSP00000031901

    UniGene: Mm.135734