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  4. Recombinant Mouse Tubulin beta-3 chain (Tubb3)

Recombinant Mouse Tubulin beta-3 chain (Tubb3)

  • 货号：
    CSB-YP875352MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP875352MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP875352MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP875352MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP875352MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tubb3; Tubulin beta-3 chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-450
  • 氨基酸序列
    MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPSGNYVGDS DLQLERISVY YNEASSHKYV PRAILVDLEP GTMDSVRSGA FGHLFRPDNF IFGQSGAGNN WAKGHYTEGA ELVDSVLDVV RKECENCDCL QGFQLTHSLG GGTGSGMGTL LISKVREEYP DRIMNTFSVV PSPKVSDTVV EPYNATLSIH QLVENTDETY CIDNEALYDI CFRTLKLATP TYGDLNHLVS ATMSGVTTSL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTARGSQQ YRALTVPELT QQMFDAKNMM AACDPRHGRY LTVATVFRGR MSMKEVDEQM LAIQSKNSSY FVEWIPNNVK VAVCDIPPRG LKMSSTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEGEMYEDD DEESEAQGPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.
  • 基因功能参考文献：
    1. Here, we report that dissociation of UNC5C and polymerized TUBB3, the highly dynamic beta-tubulin isoform in neurons, is essential for netrin-1/UNC5C-promoted axon repulsion. These results not only provide a working model of direct modulation of MTs by guidance cues in growth cone navigation but also help us to understand molecular mechanisms underlying developmental brain disorders associated with TUBB3 mutations. PMID: 28483977
    2. Tubb3 could be regulated by both Akt and Erk pathways. PMID: 26373733
    3. It findings reveal that expression of relatively low levels of TUBB3 in mitotic cells can be detrimental for their cytokinesis and underscore the importance of CIT-K in counteracting this event. PMID: 26586574
    4. Results suggest that down syndrome cell adhesion molecule collaborates with deleted in colorectal cancer to regulate microtubule dynamics via direct binding to dynamic TUBB3 in Netrin-1-induced axon branching. PMID: 25754961
    5. Study shows the critical and specific role of Tubb3 on the stereotyped morphological changes and polarization processes that are required for initiating radial migration to the cortical plate. PMID: 24179174
    6. this study reports that TUBB3, the most dynamic beta-tubulin isoform in neurons, directly interacts with the netrin receptor DCC, and that netrin-1 induces this interaction in primary neurons. PMID: 23641072
    7. Access to a running wheel for 48h induced plastic changes in the expression of astrocyte beta-tubulin (III). Expression was increased in the cerebellum while much smaller increases were observed in the other motor regions. PMID: 23201358
    8. interaction between alpha-syn and beta-III tubulin plays an important role in neuronal alpha-syn accumulation in an multiple system atrophy mouse model. PMID: 22227187
    9. Expression of Tubb3, a beta-tubulin isotype, is regulated by androgens in mouse and rat Sertoli cells. PMID: 21734264
    10. these findings indicate that TGF-beta stimulated aberrant expression of class III beta-tubulin via activation of the ERK signaling pathway. PMID: 22037456
    11. Endotatin can inhibit the expression of Tubulin-beta in retinal neovascularization. PMID: 20067108
    12. High betaIII-tubulin is associated with tumorigenesis in non-small cell lung cancer. PMID: 20501838
    13. Findings demonstrate that normal TUBB3 is required for axon guidance and maintenance in mammals. PMID: 20074521
    14. TNF produced by activated glial cells in inflammatory or degenerative neurological diseases acts on neurites by acting on the kinesin-tubulin complex and inhibits axonal mitochondria and synaptophysin transport via JNK. PMID: 17068110
    15. Transgenic mouse embryos overexpressing Wnt7a specifically in nestin-positive neural stem/progenitor cells displayed a delay in neuronal differentiation, assayed by beta-tubulin III expression. PMID: 17174942
    16. A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein. PMID: 17868115
    17. The expression of class III beta-tubulin was not related to retinal cell morphology or cell function, but rather to the cell lineage, suggesting that the expression of beta-tubulin III may be due to the retinal cell specific developmental program. PMID: 17900541

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium.
  • 蛋白家族：
    Tubulin family
  • 组织特异性：
    Expressed in the external granular layer cells of the cerebellar cortex (at protein level).
  • 数据库链接：

    KEGG: mmu:22152

    STRING: 10090.ENSMUSP00000071134

    UniGene: Mm.40068