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  4. Recombinant Mouse Twist-related protein 1 (Twist1)

Recombinant Mouse Twist-related protein 1 (Twist1)

  • 中文名称：
    小鼠Twist1重组蛋白
  • 货号：
    CSB-YP025358MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Twist1重组蛋白
  • 货号：
    CSB-EP025358MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Twist1重组蛋白
  • 货号：
    CSB-BP025358MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Twist1重组蛋白
  • 货号：
    CSB-MP025358MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Twist1
  • Uniprot No.：
  • 别名：
    Twist1; Twist; Twist-related protein 1; M-twist
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-206
  • 氨基酸序列
    MMQDVSSSPV SPADDSLSNS EEEPDRQQPA SGKRGARKRR SSRRSAGGSA GPGGATGGGI GGGDEPGSPA QGKRGKKSAG GGGGGGAGGG GGGGGGSSSG GGSPQSYEEL QTQRVMANVR ERQRTQSLNE AFAALRKIIP TLPSDKLSKI QTLKLAARYI DFLYQVLQSD ELDSKMASCS YVAHERLSYA FSVWRMEGAW SMSASH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer.
  • 基因功能参考文献：
    1. Overexpression of Twist1 in mouse muscle progenitor cells, either constitutively during development or inducibly in adult animals, caused severe muscle atrophy with features reminiscent of cachexia. PMID: 29920276
    2. This study evaluated the role of Twist1 in the expression of other epithelial-mesenchymal transition transcription factors in tumor cells, including tumor progression, intravasation, and metastasis. PMID: 29073077
    3. The s demonstrate that Twist1 serine (Ser) 42 phosphorylation is required for endothelial-to-mesenchymal transition through TGF-beta-Smad signaling in vitro and in the mouse lung gel implantation system. PMID: 28915063
    4. Overall, hypoxia-induced activation of Twist/miR-214/E-cadherin axis is involved in the EMT of TECs, and anti-miR-214 may be an attractive strategy to ameliorate the progression of renal fibrosis. PMID: 29277613
    5. molecular and cellular processes that regulate dural Cerebral vein development in mammals and describe venous malformations in humans with craniosynostosis and TWIST1 mutations that are recapitulated in mouse models, are reported. PMID: 28844842
    6. Methyltransferase G9A Regulates Osteogenesis via Twist Gene Repression in mice. PMID: 28644763
    7. this study shows that loss of Twist1 in collagen-producing cells leads to increased bleomycin-induced pulmonary fibrosis, which is mediated by increased expression of CXCL12 PMID: 28179498
    8. RNF8-promoted Twist ubiquitination is required for Twist localization to the nucleus for subsequent epithelial-mesenchymal transition and cancer stem cells functions, thereby conferring chemoresistance. PMID: 27618486
    9. These results indicate that Twist1 Ser42 phosphorylation contributes to the pathogenesis of bleomycin-induced pulmonary fibrosis through angiopoietin-Tie2 signaling. PMID: 27281171
    10. the mesenchymal properties of the cranial mesoderm are likely to be regulated by a network of TWIST1 targets that influences the extracellular matrix and cell-matrix interactions, and collectively they are required for the morphogenesis of the craniofacial structures. PMID: 27546376
    11. TWIST1 expression promotes developmental angiogenesis by inducing endothelial cell proliferation and migration. PMID: 27245171
    12. There was a possible regulatory link between Twist 1 and PPARgamma in 3T3-L1 mature adipocytes. This regulatory link enhanced the regulation of PPARgamma and may be a functional mechanism of Twist 1 regulation of adipocyte physiology and pathology PMID: 27825360
    13. ur study revealed the dynamic Twist localization within the early stage of embryo. The results are discussed in terms of potential roles of Twist1 in the processes of lineage segregation, hatching, and implantation in post-compaction embryos and in blastocysts. PMID: 27544279
    14. Twist1, a target of canonical Wnt/beta-catenin signaling, also functions to maintain Wnt responsiveness and is a key effector for cranial bone fate selection and dermal condensation. PMID: 26677825
    15. Findings indicate that Twist1 has a novel role in epithelial carcinogenesis by regulating proliferation of keratinocytes, including keratinocyte stem cells during tumor promotion. PMID: 26013710
    16. Data show that administration of MVA-TWIST/TRICOM vaccine induces Twist transcription factor-specific T-cell responses. PMID: 26317648
    17. studies suggested that the Twist1 transcription in GC cells might be regulated through potential cooperation of DNA methylation, histone modification in complex with Sp1 binding to CpG-rich regions within the exon 1 region PMID: 26695186
    18. Study shows that Twist1 plays a pivotal role in the TNF mediated suppression of E-box dependent transactivation of Period genes and Dbp. Thereby Twist1 may provide a link between the immune system and the circadian timing system. PMID: 26361389
    19. Hypoxia models showed the mechanism by which hypoxia promoted Twist1 and Bmi1 expression and led to vasculogenic mimicry formation. PMID: 26202447
    20. Twist1 plays crucial roles in regulating tooth development and it may exert its functions through the FGF signaling pathway PMID: 26487719
    21. Overexpression and cooperation of Bcl-2 and Twist1 is associated with epithelial-mesenchymal transition and vasculogenic mimicry formation in hepatocellular carcinoma. PMID: 26318343
    22. These results indicate that tamoxifen can significantly accelerate Twist1 degradation to suppress cancer cell invasion and metastasis, regardless of the estrogen receptor status. PMID: 25892968
    23. overexpression of Id2 in primary alveolar epithelial cells promotes proliferation by inhibiting a retinoblastoma protein/c-Abl interaction leading to greater c-Abl activity. PMID: 25661109
    24. the levels of twist1 in circulating exosomes from fibrotic mice reflected fibrosis-induced changes in the liver itself PMID: 26229009
    25. Twist-1 regulate hematopoietic stem cell self-renewal and myeloid lineage development PMID: 25100001
    26. Notch1 inhibits chondrogenic differentiation of mesenchymal progenitor cells by binding the Twist1 promoter. PMID: 25596548
    27. Twist1 is required for skin tumor initiation and progression in a gene-dosage-dependent manner. PMID: 25575080
    28. in repeatedly activated murine Th1 cells, Twist1 and T-bet induce expression of microRNA-148a and control the differentiation and function of Th1 cells PMID: 25486906
    29. Findings define Twist1 as a compelling target to deprogram the tumor-supporting features of the cancer microenvironment. PMID: 25368021
    30. Twist 1 is a regulator of adipocyte gene expression although it is not likely to regulate differentiation. PMID: 25128964
    31. DEC2 regulates cellular function by modulating the expression of Twist1 PMID: 25446074
    32. Hyaluronan overproduction allows plastic cancer cell populations to revert to stem cell states via Twist and the TGF-beta-Snail signaling axis. PMID: 25077968
    33. Twist1(+/-) mice have a consistent pattern of craniofacial dysmorphology affecting all major regions of the skull. PMID: 24585549
    34. Twist1 is a key downstream effector of p62 in regulation of cell proliferation and migration PMID: 24927592
    35. TWIST reverses muscle cell differentiation through promoter binding and down-regulation of myogenin. PMID: 24188104
    36. Twist1 induces dramatic transcriptional changes in extracellular compartment and cell-matrix adhesion genes but not in cell-cell adhesion genes. PMID: 24590176
    37. Twist1 is not essential for maintaining an overall healthy condition in young and adult mice and that loss of function facilitates hair growth in adulthood. PMID: 23906809
    38. Twist and miR-34a may regulate the effect of tumor cells. PMID: 24129179
    39. The proto-oncogene TWIST1 is regulated by microRNAs. PMID: 23741524
    40. identify the transcription factor Twist1 as a component of a STAT3-induced feedback loop that controls IL-6 signals by directly repressing Il6ra. PMID: 23935104
    41. dissimilarities between Twist1(+/+) and Twist1(+/-) mice are not limited to a fused coronal suture but include differences in fusion of other sutures PMID: 22903506
    42. These data demonstrate that Twist1 functions in post-migratory neural crest cells to repress pro-neural factors and thereby regulate cell fate determination between ectodermal and mesodermal lineages. PMID: 23555309
    43. haploinsufficiency of TCF12 causes coronal synostosis in humans and that severe bilateral coronal synostosis occurs in mice with 50% of the wild-type dosage of both the Tcf12 and Twist1 genes highlights the key role of TCF12 acting with TWIST1. PMID: 23354436
    44. Findings indicate a role of Twist1 in maintaining the mesenchyme architecture and the progenitor state of the mesoderm, as well as mediating mesoderm-neural crest interactions in craniofacial development. PMID: 23261931
    45. Twist1 contributes to transcriptional transactivation in mesenchymal, but not in pancreatic, cells PMID: 23181698
    46. The findings suggest that Runx2 haploinsufficiency might result in excessive unbound Twist1 that can freely bind to E12 and enhance FGF signaling, thereby promoting the formation of extra teeth. PMID: 22972545
    47. TWIST1 can exert its function by direct DNA binding to activate valve specific gene expression. PMID: 22815831
    48. These findings provide insight into how beta-catenin signaling via Twist1 actively suppresses the formation of cartilage and promotes intramembranous ossification in the skull. PMID: 23095887
    49. PROX1 directly binds to proximal promoter of TWIST1 gene to repress its transcription PMID: 22982861
    50. the suppression of Twist1 to physiological levels is sufficient to cause Kras mutant lung tumors to undergo senescence and lose their neoplastic features PMID: 22654667

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Subset of mesodermal cells.
  • 数据库链接：

    KEGG: mmu:22160

    STRING: 10090.ENSMUSP00000040089

    UniGene: Mm.3280