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  4. Recombinant Mouse Tyrosine-protein kinase BTK (Btk)

Recombinant Mouse Tyrosine-protein kinase BTK (Btk)

  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase BTK(Btk),Yeast
  • 货号：
    CSB-YP002867MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase BTK(Btk),Yeast
  • 货号：
    CSB-EP002867MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase BTK(Btk),Yeast
  • 货号：
    CSB-EP002867MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase BTK(Btk),Yeast
  • 货号：
    CSB-BP002867MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Tyrosine-protein kinase BTK(Btk),Yeast
  • 货号：
    CSB-MP002867MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Btk; BpkTyrosine-protein kinase BTK; EC 2.7.10.2; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; Kinase EMB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-659
  • 氨基酸序列
    AAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVIPE KNPPPERQIP RRGEESSEME QISIIERFPY PFQVVYDEGP LYVFSPTEEL RKRWIHQLKN VIRYNSDLVQ KYHPCFWIDG QYLCCSQTAK NAMGCQILEN RNGSLKPGSS HRKTKKPLPP TPEEDQILKK PLPPEPTAAP ISTTELKKVV ALYDYMPMNA NDLQLRKGEE YFILEESNLP WWRARDKNGQ EGYIPSNYIT EAEDSIEMYE WYSKHMTRSQ AEQLLKQEGK EGGFIVRDSS KAGKYTVSVF AKSTGEPQGV IRHYVVCSTP QSQYYLAEKH LFSTIPELIN YHQHNSAGLI SRLKYPVSKQ NKNAPSTAGL GYGSWEIDPK DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIREGSMSED EFIEEAKVMM NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER FTNSETAEHI AQGLRLYRPH LASERVYTIM YSCWHEKADE RPSFKILLSN ILDVMDEES
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
  • 基因功能参考文献：
    1. CD40-mediated signals, likely from CD4 T cells, can mediate peripheral transitional B cell maturation independent of Btk and the non-canonical NF-kappaB pathway. PMID: 28378771
    2. As chronic lymphocytic leukemia (CLL) proliferation and survival depends on the microenvironment, we used murine models to assess the efficacy of the BTK inhibitor acalabrutinib combined with the PI3Kdelta inhibitor ACP-319 in vivo.The combination of acalabrutinib and ACP-319 was superior to single-agent treatment in a murine CLL model PMID: 28645939
    3. Results show that BTK adopts a closed conformation in dynamic equilibrium with open, active conformations. BTK lacks the phosphotyrosine regulatory tail of the SRC kinases, yet nevertheless achieves a phosphotyrosine-independent C-terminal latch. The unique proline-rich region is an internal "on" switch pushing the autoinhibited kinase toward its active state. PMID: 28867612
    4. acalabrutinib showed increased BTK selectivity compared with ibrutinib while demonstrating significant antitumor efficacy in vivo on par with ibrutinib. Similarly, in the TCL1 adoptive transfer model, decreased phosphorylation of BTK, PLCgamma2, and S6 was observed. Most notably, treatment with acalabrutinib resulted in a significant increase in survival compared with mice receiving vehicle PMID: 27903679
    5. these data show that BTK is a critical NLRP3 inflammasome regulator PMID: 28216434
    6. Rictor positively regulates B cell receptor signaling via up-regulating Btk and down-regulating SH2-containing inositol phosphatase PMID: 28821013
    7. The pre-B-cell receptor (pre-BCR) signaling molecules BLNK, BTK and BANK1 were positively regulated by the ZFP521 gene, leading to enhancement of the pre-BCR signaling pathway. PMID: 26522721
    8. these data indicate that in mature B cells, Tec and Btk may compete for activation of the Akt signaling pathway, whereby the activating capacity of Btk is limited by the presence of Tec kinase. PMID: 28275136
    9. these data demonstrate that enhanced BTK signaling in B cells can establish a T cell-driven proinflammatory loop resulting in autoimmune pathology, making BTK inhibition an attractive therapeutic strategy PMID: 27226091
    10. Inhibition of Btk by inhalation of aerosolized RN983 may be effective as a stand-alone asthma therapy. PMID: 27111445
    11. Btk and NLRP3 co-regulate platelet activation, aggregation, and in vitro thrombus formation.The NLRP3 inflammasome is upregulated in activated platelets. PMID: 28034752
    12. Btk inhibition ameliorated hepatocellular injury in a well-established model of liver partial warm ischemia and in situ reperfusion. PMID: 27820779
    13. report that BTK is expressed by murine and human MDSCs, and that ibrutinib is able to inhibit BTK phosphorylation in these cells PMID: 26880800
    14. ITK and BTK regulate thermal homeostasis during septic response through mast cell function in mice. PMID: 26581914
    15. Btk inhibition treats TLR7/IFN driven murine lupus PMID: 26821304
    16. we have identified a neutrophil Btk signalosome that is involved in a signaling pathway and neutrophil recruitment during sterile inflammation PMID: 26777396
    17. Data show that Bruton's tyrosine kinase knockout (Btk-/-) mice displayed severely reduced numbers of macrophages in the colon. PMID: 25379804
    18. inositol hexakisphosphate (IP6), a soluble signaling molecule found in both animal and plant cells, also activates Btk. PMID: 25699547
    19. Data indicate interactions between Ets1 transcription factor and two of its important regulators: Lyn, which maintains Ets1 expression to limit the differentiation of autoreactive plasma cells (PCs), and Btk. PMID: 26209625
    20. SH3/SH2 interaction domains of BTK binds, respectively, the DEAD-box domain of DDX41 and transmembrane region of STING. PMID: 25704810
    21. for Aspergillus fumigatus, activation of macrophage calcineurin-NFAT occurs via a phagosomal TLR9-dependent and Bruton's tyrosine kinase-dependent signalling pathway that is independent of MyD88 PMID: 25637383
    22. BTK synergizes with Notch2 to govern marginal zone B cells in non-obese diabetic mice. PMID: 26034172
    23. novel role for BTK in lethal sepsis PMID: 24906236
    24. BTK and protein kinase C mu, that are involved in TLR7/9-triggered IFN-beta production in dendritic cells. PMID: 25170774
    25. these results suggest that PU.1 and Spi-B activate Btk to oppose IL-7 responsiveness in developing B cells. PMID: 25505273
    26. These results strongly suggest that the association between the WASP N-terminal domain and Btk plays an important role in the TLR2-signaling pathway in macrophages. PMID: 25213142
    27. present findings indicate that silencing Btk in alveolar neutrophils has a dramatic protective effect in mice with LPS/IC-induced ALI PMID: 25085625
    28. These results demonstrate an essential role for Btk-c-Src signaling in TIM-3-induced DC suppression. PMID: 25172495
    29. The role of Btk in M1/M2 macrophage polarity induction, was examined. PMID: 24465735
    30. These data define the role of BTK in the mature immune system and mechanistically predict the clinical phenotype of chronic BTK inhibition. PMID: 24899507
    31. BTK is a critical kinase for chronic lymphocytic leukemia development and expansion and thus an important target of ibrutinib. PMID: 24311722
    32. autoreactive B cells rely on BTK-mediated signaling for maturation or survival, but they can still traffic to inflamed tissues, internalize Ag, and promote cell-mediated autoimmunity without it. PMID: 24453243
    33. Data show that substitution of 6 of the 619 amino acid residues of Itk (interleukin-2-inducible T cell kinase) with the corresponding residues of Btk (Bruton's tyrosine kinase), and vice versa, completely switched the activities of Itk and Btk. PMID: 23982207
    34. new insights into the role of BTK, an important target for autoimmune diseases, in B cell activation PMID: 23967355
    35. Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to Dectin1-dependent phagocytosis of Candida albicans in macrophages. PMID: 23825946
    36. Btk-sufficient RF(+) B cells mature into naive FO B cells. PMID: 23804807
    37. The activated serine/threonine kinase Akt/protein kinase B phosphorylated BTK on two sites prior to 14-3-3 zeta binding. PMID: 23754751
    38. These data suggest an important regulatory role for Btk in macrophages during Lm infection. PMID: 23544144
    39. data revealed a novel regulatory role for Btk in mediating apoptotic cell clearance, with calreticulin identified as the critical component of the CRT/CD91/C1q system targeted by Btk. PMID: 23596312
    40. These observations strongly suggest that the phosphorylation of WASP by Btk plays a pivotal role in transducing the LPS signaling pathway in macrophages. PMID: 22634306
    41. mapped the interaction site to the C terminus of the Btk SH3 domain PMID: 22527282
    42. Transgenic mice overexpressing Btk specifically in B cells spontaneously formed germinal centers and manifested increased plasma cell numbers, leading to antinuclear autoantibody production and systemic lupus erythematosus (SLE)-like autoimmune pathology. PMID: 22383797
    43. BTK plays a critical role in initiating TLR3 signaling. PMID: 22454496
    44. These observations strongly suggest that the interaction between WASP N-terminal domain and Btk plays important roles in the lipopolysaccharide signaling cascade in innate immunity. PMID: 22253930
    45. Reduced levels of IL-12, IL-18 and IFN-gamma were observed in Btk-deficient mice and ex vivo generated macrophages and dendritic cells (DCs) following acute LPS administration PMID: 22281426
    46. a novel role for Btk in HGF-induced DC inhibition. PMID: 21784852
    47. our data identify Btk as a positive regulator in the ITAM-mediated TREM-1/DAP12 pathway and suggest its implication in inflammatory processes. PMID: 21659545
    48. These data underline the complex role of Btk in the regulation of macrophage survival and function. PMID: 21193961
    49. The Btk gene, which is mutated in human immunodeficiency X-linked agammaglobulinemia, is involved in upregulation of heme oxygenase-1 gene expression via Toll-like receptor (TLR) signaling in mouse alveolar macrophages. PMID: 21677132
    50. Determined the X-ray crystal structures of BTK kinase domain in complex with six inhibitors from distinct chemical classes. PMID: 21280133

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  • 相关疾病：
    Defects in Btk are the cause of murine X-linked immunodeficiency (XID).
  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, TEC subfamily
  • 数据库链接：

    KEGG: mmu:12229

    STRING: 10090.ENSMUSP00000033617

    UniGene: Mm.4475