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  4. Recombinant Mouse Tyrosine-protein kinase Fyn (Fyn)

Recombinant Mouse Tyrosine-protein kinase Fyn (Fyn)

  • 货号：
    CSB-YP009101MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009101MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009101MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009101MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009101MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fyn; Tyrosine-protein kinase Fyn; EC 2.7.10.2; Proto-oncogene c-Fyn; p59-Fyn
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-537
  • 氨基酸序列
    GCVQCKDKE AAKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY NNFHAAGGQG LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFHKGEKFQ ILNSSEGDWW EARSLTTGET GYIPSNYVAP VDSIQAEEWY FGKLGRKDAE RQLLSFGNPR GTFLIRESET TKGAYSLSIR DWDDMKGDHV KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC RLVVPCHKGM PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMSKGSL LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling.
  • 基因功能参考文献：
    1. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins. PMID: 28533542
    2. Fyn and Lyn as important factors that promote Plasmacytoid dendritic cell responses. PMID: 28368000
    3. these results suggest that Nrf2 plays a central role in the prevention of Ang II-induced cardiomyopathy, and SFN prevents Ang II-induced cardiomyopathy partially via the Akt/GSK-3beta/Fyn-mediated Nrf2 activation. PMID: 29353218
    4. This study reported that suppression of Fyn expression in mouse cerebral cortex led to migration defects of both early-born and late-born neurons. PMID: 28363782
    5. The results of this study suggested the existence of a potential DEP-1-Fyn axis in the regulation of microglial functions. PMID: 27859601
    6. These results suggested that Fyn has a regulatory role in iNOS expression in astrocytes during neuroinflammatory responses. PMID: 29180007
    7. Somatodendritic accumulation of Tau in Alzheimer's disease is promoted by Fyn-mediated local protein translation. PMID: 28864542
    8. loss of Fyn in the kidney prevents unilateral ureteral obstruction-induced tubulointerstitial fibrosis, mediated by a reduction in STAT3 phosphorylation PMID: 27616741
    9. results show that Fyn kinase is an important positive effector of TGF-beta-induced chemotaxis through the control of phosphatase PP2A activity and this is relevant to pathological processes that are related to TGF-beta-dependent mast cell migration PMID: 28586109
    10. In summary,MAGI-2 and Fyn protect dendrin from Nedd4-2-mediated ubiquitination and from nuclear translocation, thereby maintaining the physiologic homeostasis of podocytes. PMID: 28539383
    11. FYN is activated by oxidative stress and serves as a negative feedback regulator of NOX4 in cardiomyocytes during cardiac remodeling PMID: 27525436
    12. The results of this study suggested that Fyn-mediated NR2B signaling plays a critical role in regulation of prediabetic neuropathy and that the increased expression/function of NR2B subunit-containing NMDARs may contribute to the progression of neuropathy in type 2 diabetes PMID: 27146985
    13. Fyn acts as a regulatory nexus between solar UV, ROS and signal transduction during skin carcinogenesis. PMID: 26686094
    14. We show that PTP-3, a LAR homolog in Caenorhabditis elegans, participates in Sema2A-regulated axon guidance. PTPdelta, a member of vertebrate LAR class PTPs, is involved in Sema3A-regulated cortical dendritic growth. In Sema3A signaling, PTPdelta activates Fyn and Src kinases by dephosphorylating their C-terminal Tyr residues. PMID: 28637841
    15. data show that Fyn kinase is activated after TLR4 triggering and exerts an important negative control on LPS-dependent TNF production in mast cells controlling the inactivation of PP2Ac and activation of PKCalpha/beta necessary for the secretion of TNF by VAMP3(+) carriers PMID: 27183589
    16. Fyn deficiency alters retinal morphology and adhesion properties of Muller cells. Fyn deficiency parallels a decreased optokinetic response to visual stimuli in vivo. PMID: 26808221
    17. Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation PMID: 26881253
    18. MOBP synthesis is stimulated by Fyn activity. PMID: 26801084
    19. Fyn expression was elevated in abortion-prone mice PMID: 26892111
    20. Fyn facilitates mitotic spindle formation through the increase in microtubule polymerization, resulting in the acceleration of M-phase progression. PMID: 26365631
    21. These results suggest the critical physiological role of TRL4-Fyn interaction in the modulation of PAI-1-tPA axis in astrocytes during neuroinflammatory responses such as ischemia/reperfusion injuries. PMID: 25106729
    22. total levels of ApoER2 and surface levels of ApoER2 are increased by active Fyn PMID: 25340851
    23. Thy-1 facilitates the recruitment of membrane raft-residing signaling molecules such as Fyn kinase and the SFK regulator, Csk binding protein (Cbp), to focal adhesions via its direct coupling of integrins and raft domains. PMID: 26459603
    24. The SH2 domain of Fyn plays an essential role in Reelin signaling transduction, and an SH2 domain mutant disturbs neuronal migration in a dose-dependent manner. PMID: 24912779
    25. A synergistic regulation of Fyn signaling was found in corpus striatum regulated by protein tyrosine phosphate. PMID: 25951993
    26. This study demonstrate that both the tyrosine kinase Fyn and the serine/threonine kinase PKCdelta are differentially expressed in microglia and astrocytes. PMID: 26157004
    27. The expression of Fyn is protective in acute chemical-induced colitis and promotes recovery from disease. PMID: 25877924
    28. The aspartic acid of Fyn at 390 is indispensable for the radial migration. PMID: 25251774
    29. The normal levels of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate observed in the FynKO liver extracts suggested normal triose kinase function PMID: 24312371
    30. identify Fyn-centric gene networks influencing variance in ethanol LORR. Such networks may also inform future design of pharmacotherapies for the treatment and prevention of alcohol use disorders PMID: 24312422
    31. SAP plays an essential role in CIA because of Fyn-independent and Fyn-dependent effects on TFH cells and, possibly, other T cell types. PMID: 24045941
    32. Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells. PMID: 24036998
    33. Fyn kinase is required for optimal humoral responses PMID: 23593269
    34. cAMP-dependent protein kinase (PKA)/Fyn/GluN2-containing NMDA receptor GluN2BR signaling plays a critical role in triggering GluN2BR hyperfunction and pain hypersensitivity during inflammation. PMID: 21054385
    35. Our findings suggest a role for Fyn kinase in neuronal death after neonatal hypoxic-ischemic injury PMID: 23127881
    36. Data from knockout mice suggest that Fyn is important regulator of whole-body integrative metabolism that coordinates adipose tissue expansion, inflammation, and insulin sensitivity in states of nutrient excess (i.e., diet-induced obesity). PMID: 23321073
    37. Overexpression and activity of Fyn mediate proliferation and blastic features of chronic myelogenous leukemia. PMID: 23284724
    38. Fyn regulates the activity of the adipogenic transcription factor signal transducer and activator of transcription 5a (STAT5a) through enhancing its interaction with the GTPase phosphoinositide 3-kinase enhancer A (PIKE-A). PMID: 23438599
    39. Mouse skeletal muscle fiber-type-specific macroautophagy and muscle wasting are regulated by a Fyn/STAT3/Vps34 signaling pathway. PMID: 22745922
    40. Phosphorylation of cytohesin-1 by Fyn is required for initiation of myelination and the extent of myelination during development. PMID: 23012656
    41. mechanism underlying impaired TCR signaling and subsequent memory cell anergy must involve a Fyn signaling pathway given that the suppression of Fyn activity restores CD3/ZAP-70 complex formation and TCR proximal signaling PMID: 22386537
    42. our results demonstrate that Fyn kinase downregulates nonmuscle myosin II activity, thus promoting oligodendrocyte morphological differentiation. PMID: 22437915
    43. Fyn is identified as a possible novel regulator of the developmental balance between T helper (Th)17 type and regulatory T (TREG) cell subsets. PMID: 22539787
    44. Fyn relays RANK/RANKL signal to the costimulatory DAP12/FcRgamma-mediated signals. PMID: 22387224
    45. Fyn is not required for the pathogenesis of Bcr-Abl-mediated leukemias. PMID: 22189847
    46. Lyn but not Fyn kinase controls IgG-mediated systemic anaphylaxis. PMID: 22450804
    47. loss of Fyn at early stages of disease increases soluble Abeta accumulation and worsens spatial learning in the absence of changes in tau phosphorylat in Alzheimer's disease. PMID: 21741124
    48. These findings are consistent with a model in which FYN kinase plays an important role in proper shaping of the head and acrosome within the testis and possibly an additional role in the sperm acrosome reaction. PMID: 21918125
    49. The data of this study indicated that Fyn differentially affects spine density and morphology in the cortex and hippocampus and has age-dependent effects on dendritic spine formation and spine morphology in vivo PMID: 21872217
    50. TM4SF10, possibly through ADAP, may regulate Fyn activity PMID: 21881001

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Isoform 1 is highly expressed in the brain, isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
  • 数据库链接：

    KEGG: mmu:14360

    STRING: 10090.ENSMUSP00000097547

    UniGene: Mm.4848