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  4. Recombinant Mouse Tyrosine-protein kinase HCK (Hck)

Recombinant Mouse Tyrosine-protein kinase HCK (Hck)

  • 货号：
    CSB-YP010211MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010211MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010211MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010211MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010211MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    HckTyrosine-protein kinase HCK; EC 2.7.10.2; B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-524
  • 氨基酸序列
    GGRSSCEDP GCPRSEGRAP RMGCVKSRFL RDGSKASKTE PSANQKGPVY VPDPTSSSKL GPNNSNSMPP GFVEGSEDTI VVALYDYEAI HREDLSFQKG DQMVVLEEAG EWWKARSLAT KKEGYIPSNY VARVNSLETE EWFFKGISRK DAERHLLAPG NMLGSFMIRD SETTKGSYSL SVRDFDPQHG DTVKHYKIRT LDSGGFYISP RSTFSSLQEL VLHYKKGKDG LCQKLSVPCV SPKPQKPWEK DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF LAEANLMKSL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK QPLPKLIDFS AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS NPEVIRALEH GYRMPRPDNC PEELYNIMIR CWKNRPEERP TFEYIQSVLD DFYTATESQY QQQP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
  • 基因功能参考文献：
    1. Hematopoietic Hck promotes tumorigenesis. PMID: 28399411
    2. Data suggest that Hck is a key mediator of renal fibrosis PMID: 27927780
    3. Hck is not only progressively overexpressed in atherosclerosis, but it also controls critical molecular processes in monocyte influx, blood monocyte subset balance, macrophage accumulation, and the maintenance of atherosclerotic lesion stability. PMID: 26068045
    4. tyrosine phosphorylation of WASP by Hck is required for proper macrophage functions PMID: 24482227
    5. Hck is one of the very few effectors of preosteoclast recruitment described to date and thereby plays a critical role in bone remodeling. PMID: 23742809
    6. Despite possessing enhanced killing, alveolar macrophage Hck/Fgr/Lyn-deficient ("triple-knockout") mice do not demonstrate enhanced inflammatory responses to Pneumocystis murina. PMID: 21220696
    7. Taken together, PKR and Hck were critical for DON-induced ribosomal recruitment of p38, its subsequent phosphorylation, and, ultimately, p38-driven proinflammatory cytokine expression. PMID: 20181660
    8. Nef participates in HIV-1-induced multinucleated giant cells formation via a p61Hck- and lysosomal enzyme-dependent pathway PMID: 20488787
    9. spatial organization of podosomes as large rosettes, proteolytic degradation of extracellular matrix, and 3D migration appeared to be functionally linked and regulated by Hck in macrophages PMID: 19897576
    10. enhanced innate immune response in Hck(F/F) mice thereby skewing innate immunity from a reversible physiological host defense response to one causing irreversible tissue damage PMID: 12208875
    11. BCR/ABL SH3 and SH2 domains interact with hematopoietic cell kinase (Hck), leading to the stimulation of Hck catalytic activity PMID: 12411494
    12. Hck plays no role in chemoattractant-induced, inside-out beta 2 integrin activation of neutrophils but regulates their outside-in, signaling-dependent sustained adhesion. PMID: 16785558
    13. Lyn activity is elevated in hck(-/-) cells, leading to increased phosphorylation of several negative regulators PMID: 17513616
    14. a myeloproliferative transformation-sensitive signaling pathway, composed of Lyn/Hck, SHIP, autocrine/paracrine cytokines, and Stat5, that regulates HSC differentiation and M2 macrophage programming. PMID: 18246197
    15. Alternate protein isoforms arise through the use of a non-AUG (CUG), and a downstream in-frame AUG, translation initiation codons, in mouse and human. PMID: 1875927

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.; [Isoform 1]: Membrane; Lipid-anchor. Membrane, caveola. Lysosome. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol.; [Isoform 2]: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • 数据库链接：

    KEGG: mmu:15162

    STRING: 10090.ENSMUSP00000003370

    UniGene: Mm.715