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  4. Recombinant Mouse Tyrosine-protein kinase ZAP-70 (Zap70)

Recombinant Mouse Tyrosine-protein kinase ZAP-70 (Zap70)

  • 中文名称：
    小鼠Zap70重组蛋白
  • 货号：
    CSB-YP026303MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Zap70重组蛋白
  • 货号：
    CSB-EP026303MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Zap70重组蛋白
  • 货号：
    CSB-EP026303MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Zap70重组蛋白
  • 货号：
    CSB-BP026303MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Zap70重组蛋白
  • 货号：
    CSB-MP026303MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Zap70; Srk; Zap-70; Tyrosine-protein kinase ZAP-70; EC 2.7.10.2; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-618
  • 氨基酸序列
    MPDPAAHLPF FYGSISRAEA EEHLKLAGMA DGLFLLRQCL RSLGGYVLSL VHDVRFHHFP IERQLNGTYA IAGGKAHCGP AELCQFYSQD PDGLPCNLRK PCNRPPGLEP QPGVFDCLRD AMVRDYVRQT WKLEGDALEQ AIISQAPQVE KLIATTAHER MPWYHSSLTR EEAERKLYSG QQTDGKFLLR PRKEQGTYAL SLVYGKTVYH YLISQDKAGK YCIPEGTKFD TLWQLVEYLK LKADGLIYRL KEVCPNSSAS AAVAAPTLPA HPSTFTQPQR RVDTLNSDGY TPEPARLASS TDKPRPMPMD TSVYESPYSD PEELKDKKLF LKRENLLVAD IELGCGNFGS VRQGVYRMRK KQIDVAIKVL KQGTEKADKD EMMREAQIMH QLDNPYIVRL IGVCQAEALM LVMEMAGGGP LHKFLLGKKE EIPVSNVAEL LHQVAMGMKY LEEKNFVHRD LAARNVLLVN RHYAKISDFG LSKALGADDS YYTARSAGKW PLKWYAPECI NFRKFSSRSD VWSYGVTMWE AFSYGQKPYK KMKGPEVLDF IKQGKRMECP PECPPEMYAL MSDCWIYKWE DRPDFLTVEQ RMRNYYYSLA SRAEGPPQCE QVAEAACG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
  • 基因功能参考文献：
    1. hypomorphic alleles of Zap70 or chemical inhibition of Zap70 catalysis leads to an increase of IFN-gamma-producing invariant natural killer T cells. PMID: 29980684
    2. RanBP2 expression was regulated by Zap70. PMID: 28745977
    3. disruption of normal Zap70 autoinhibition engaged negative feedback mechanisms by which negative selection and inhibitory receptors restrain TCR signaling to enforce both central and peripheral tolerance. PMID: 28159798
    4. in this study, s discovered a cycle of recruitment, activation and release for Zap70 kinases at phosphorylated T-cell antigen receptors, which turned them into a 'catalytic unit' that amplified antigenic stimuli PMID: 27869819
    5. was significantly increased in 22-month AEW mice compared with 5-month AEW mice. ELISA data showed that secretions of IL-2 and NGF in 22-month AEW mice were higher than 5-month AEW mice. Our results indicate that increased ZAP70 is involved in dry skin in elderly pruritus. Increased secretion of IL-2 and NGF may induce dry skin itch. PMID: 27195291
    6. this study shows that ZAP-70 mutation has a strong impact on T cell-driven arthritis in SKG model regardless of the genetic background PMID: 27040161
    7. These findings establish Otud7b as a positive regulator of T cell receptor-proximal signaling and T cell activation, highlighting the importance of deubiquitination in regulating Zap70 function. PMID: 26903241
    8. Data suggest that ring finger protein 41 Nrdp1 terminates T cell antigen receptors (TCRs) signaling by inactivating Zap70 kinase. PMID: 26390156
    9. Zap70 as a structural protein regulates integrin-mediated control of actin and with its catalytic activity regulates T cell receptor-mediated control of actin and membrane remodelling during formation of the immunological synapse. PMID: 24596147
    10. Blockade of CXCR7 suppressed MIF-mediated ERK- and zeta-chain-associated protein kinase (ZAP)-70 activation PMID: 26139098
    11. mediates the CD4 lineage differentiation in response to Class II selecting ligands PMID: 25601273
    12. In mice with spondylarthritis, microbiota content varied according to whether T cell receptor signal strength was normal or was impaired due to the ZAP-70(W163C) mutation. PMID: 25048686
    13. expression of Zap70 protein was essential for both long-term survival of naive T cells and for their proliferation in response to lymphopenia. PMID: 25092893
    14. ZAP-70 enhances the migration of malignant B-cells into the supportive microenvironment found in the bone marrow mainly by enhancing signaling and migration after CXCR4 stimulation. PMID: 24312539
    15. Titration of Zap70 activity resulted in graded reductions in positive and negative selection but did not decrease the cumulative TCR signals integrated by positively selected OT-I cells PMID: 24908390
    16. MIF promotes the migration of B cells through a ZAP-70-dependent pathway mediated by cooperative engagement of CXCR4 and CD74. PMID: 24760155
    17. Report role of ZAP-70 kinase mutation/deletion on thymocyte-positive selection via CD69 signaling. PMID: 24266404
    18. The forced intrathymic administration of histocompatible hematopoietic stem cells can sustain long-term thymopoiesis in ZAP-70-immunodeficient mice. PMID: 23305740
    19. ZAP70 recruitment to the TCR and ZAP70 activation, revealed by its phosphorylation at tyrosine residues 292 and 319, were inhibited in T cells with a mutation in the proline-rich sequence of CD3 epsilon. PMID: 23267019
    20. that CD3zeta mediates the ZAP-70/Syk kinase activation triggered by ephrinA-activated pathway to regulate early neuronal morphogenesis PMID: 21895656
    21. Data observed a significant upregulation and phosphorylation of ZAP-70, protein kinase C theta;, nuclear factor of activated T cells, and glycogen synthase kinase 3beta in Tregs prepared from injury-site-draining lymph nodes. PMID: 20720513
    22. Sts-2 is a phosphatase that negatively regulates zeta-associated protein (ZAP)-70 and T cell receptor signaling pathways. PMID: 21393235
    23. Results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3zeta in the immunological synapse. PMID: 21103055
    24. These data provide evidence that a burst of ZAP-70 activity initiates the signaling pathways for negative selection. PMID: 20945976
    25. conventional naive, effector and memory T cells were dependent on the kinase activity of Zap70 for their activation, which demonstrated a nonredundant role for Zap70 in signals induced by the T cell antigen receptor PMID: 21037577
    26. temporal gradient in the amount of Zap70 protein enabled the selection of CD4(+) and CD8(+) repertoires in separate temporal windows and at different TCR signaling thresholds PMID: 20332428
    27. These ZAP-70-deficient mice that received an intrathymic transplant had a significantly increased prothymocyte niche compared with their untreated counterparts. PMID: 20040762
    28. ZAP-70 plays a role in the transition of pro-B to pre-B cells in the bone marrow. PMID: 12705855
    29. spontaneous point mutation of the gene encoding an SH2 domain of ZAP-70, a key signal transduction molecule in T cells, causes chronic autoimmune arthritis in mice that resembles human RA in many aspects PMID: 14647385
    30. ZAP-70 plays a distinct role in T cell activation and activation-induced cell death that involves the Fas/Fas ligand, caspase-8 and caspase-3 pathways. PMID: 14734724
    31. ZAP-70 is essential for T-cell receptor signaling which induces the proliferation and the final maturation of dendritic epidermal T cells in the epidermis. PMID: 15740591
    32. novel role for ZAP-70, which replaces SYK for continued t cell receptor signaling PMID: 17606633
    33. A novel biochemical signature in memory CD4 T cells enables efficacious responses through increased Zap70 expression and reduced accumulation of downstream signaling events. PMID: 17785805
    34. The pleiotropic functions of ZAP-70 and their differential response to graded variation provide a paradigm for understanding the complex outcomes of human genetic variation. PMID: 18093540
    35. The thymic electro-gene transfer of the pCMV-ZAP-70-IRES-EGFP vector in these mice resulted in rapid T cell differentiation in the thymus with mature lymphocytes detected by three weeks in secondary lymphoid organs. PMID: 18446234
    36. Zap70 can rescue the defective positive selection of immature B cells into the recirculating pool in Syk-deficient mice, demonstrating functional redundancy between these two kinases. PMID: 18465772
    37. ZAP-70 mutations appear to skew the T Cell receptor repertoire in ways that differentially influence propensity to autoimmunity versus autoimmune disease susceptibility. PMID: 19841086

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, ZAP70 is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in thymus, spleen and lymph nodes.
  • 数据库链接：

    KEGG: mmu:22637

    STRING: 10090.ENSMUSP00000027291

    UniGene: Mm.8038