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  4. Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 11 (Ptpn11)

Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 11 (Ptpn11)

  • 中文名称：
    小鼠Ptpn11重组蛋白
  • 货号：
    CSB-YP019025MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ptpn11重组蛋白
  • 货号：
    CSB-EP019025MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ptpn11重组蛋白
  • 货号：
    CSB-EP019025MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ptpn11重组蛋白
  • 货号：
    CSB-BP019025MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ptpn11重组蛋白
  • 货号：
    CSB-MP019025MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ptpn11; Tyrosine-protein phosphatase non-receptor type 11; EC 3.1.3.48; Protein-tyrosine phosphatase SYP; SH-PTP2; SHP-2; Shp2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-597
  • 氨基酸序列
    TSRRWFHPN ITGVEAENLL LTRGVDGSFL ARPSKSNPGD FTLSVRRNGA VTHIKIQNTG DYYDLYGGEK FATLAELVQY YMEHHGQLKE KNGDVIELKY PLNCADPTSE RWFHGHLSGK EAEKLLTEKG KHGSFLVRES QSHPGDFVLS VRTGDDKGES NDGKSKVTHV MIRCQELKYD VGGGERFDSL TDLVEHYKKN PMVETLGTVL QLKQPLNTTR INAAEIESRV RELSKLAETT DKVKQGFWEE FETLQQQECK LLYSRKEGQR QENKNKNRYK NILPFDHTRV VLHDGDPNEP VSDYINANII MPEFETKCNN SKPKKSYIAT QGCLQNTVND FWRMVFQENS RVIVMTTKEV ERGKSKCVKY WPDEYALKEY GVMRVRNVKE SAAHDYTLRE LKLSKVGQAL LQGNTERTVW QYHFRTWPDH GVPSDPGGVL DFLEEVHHKQ ESIVDAGPVV VHCSAGIGRT GTFIVIDILI DIIREKGVDC DIDVPKTIQM VRSQRSGMVQ TEAQYRFIYM AVQHYIETLQ RRIEEEQKSK RKGHEYTNIK YSLVDQTSGD QSPLPPCTPT PPCAEMREDS ARVYENVGLM QQQRSFR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification.
  • 基因功能参考文献：
    1. Genetic or pharmacologic inactivation of SHP2 promotes accumulation of JAK2 phosphorylated at Y570, reduces JAK2/STAT3 signaling, inhibits TGFbeta-induced fibroblast activation and ameliorates dermal and pulmonary fibrosis. PMID: 30108215
    2. These findings identify Shp2 in podocytes as a significant contributor to the signaling events following LPS challenge and suggest that inhibition of Shp2 in podocytes may present a potential therapeutic target for podocytopathies. PMID: 28352079
    3. Loss of Shp2 within radial glia resulted in cortical dysplasia with decreased ERK signaling and was associated with glial defects and impaired sensorymotor development of newborn mice. PMID: 29257282
    4. yolk sac erythromyeloid progenitors expressing mutant PTPN11 demonstrate functional and molecular features of juvenile myelomonocytic leukemia but do not cause disease following transplantation nor following unperturbed development. PMID: 28975680
    5. nuclear Shp2, rather than cytosolic Shp2, promotes the ERalpha transcription activity. This function is achieved by enhancing the Src kinase-mediated ERalpha tyrosine phosphorylation, which facilitates ERalpha binding to Pgr promoter in an ERK-independent manner in periimplantation uteri. PMID: 28424251
    6. HIF1A dependent wound healing angiogenesis in vivo can be controlled by site-specific lentiviral magnetic targeting of SHP2. PMID: 28434868
    7. High SHP2 expression prevents colitis-associated cancer development. PMID: 27582544
    8. T lymphocyte SHP2-deficiency triggers anti-tumor immunity to inhibit colitis-associated colonic cancer in mice. PMID: 27935860
    9. Here the s demonstrate a central role of Ptpn11 in postnatal myogenesis of mice. Loss of Ptpn11 drove muscle stem cells out of the proliferative and into a resting state during muscle growth. This Ptpn11 function was observed in postnatal but not fetal myogenic stem cells. Furthermore, muscle repair was severely perturbed when Ptpn11 was ablated in stem cells due to a deficit in stem cell proliferation and survival. PMID: 28463680
    10. a method that allows the localized visualization of oxidized intermediates of SHP-2 protein-tyrosine phosphatase inside cells during signaling. PMID: 28878211
    11. NO controls the calcium signal propagation through Cx37-containing gap junctions. The tyrosine phosphatase SHP-2 is the essential mediator and NO target. PMID: 29025706
    12. These results suggest that SHP-2-via association with ICAM-1-mediates ICAM-1-induced Src activation and modulates VE-cadherin switching association with ICAM-1 or actin, thereby negatively regulating neutrophil adhesion to endothelial cells and enhancing their transendothelial migration. PMID: 28701303
    13. Inactivation of ALX4/Alx4 causes lacrimal gland aplasia in both human and mouse. These results reveal a key role of Alx4 in mediating FGF-Shp2-FGF signaling in the neural crest for lacrimal gland development. PMID: 29028795
    14. Ptpn11 gain-of-function mutations are associated with myeloproliferative neoplasm. PMID: 27840422
    15. aberrant SHP2 signaling during cardiac development leads to congenital heart disease and adult-onset heart hypertrophy PMID: 27348588
    16. Data suggest that phosphorylation of Shp2/Ptpn11 at Tyr542 and its translocation to postsynaptic compartment are integral processes in synaptic scaling/homeostasis; Shp2 phosphatase activity is critical to regulation of Ser(P)845 GluA1 and surface expression of GluA1 during synaptic scaling. (Shp2/Ptpn11 = protein tyrosine phosphatase non-receptor type 11; GluA1 = glutamate receptor ionotropic Ampa1 [alpha 1]) PMID: 28768764
    17. data suggested that deletion of Shp2 impaired IL-25 production in bronchial epithelial cells in vitro, but might yet have minor influence on OVA-induced allergic reaction in vivo PMID: 28481957
    18. The effects of SHP2 overexpression and inhibition on fibroblast response to profibrotic stimuli were analyzed in in the bleomycin model of lung fibrosis by SHP2-lentiviral administration in transgenic mice carrying a constitutively active SHP2 mutation. Mice carrying the Noonan syndrome-associated SHP2 mutation were resistant bleomycin induced pulmonary fibrosis. PMID: 27736153
    19. Data show aberrant neuronal activity-induced signaling and regulation of gene expression in protein tyrosine phosphatase, non-receptor type 11 (Ptpn11D61Y) mutation and suggest that these deficits contribute to the pathophysiology of cognitive impairments in Noonan syndrome (NS). PMID: 28346493
    20. This study indicates that Shp2 is required to orchestrate macrophage function and regulate host innate immunity against pulmonary bacterial infection. PMID: 27330052
    21. In conclusion, our data suggest that SIRPalpha signaling through SHP-2-PI3K-Akt2 strongly influences osteoblast differentiation from bone marrow stromal cells. PMID: 27422603
    22. SHP-2 is functionally important for the maintenance of appropriate barrier function and host-microbiota homeostasis in the large intestine. PMID: 27100271
    23. This study reveals the critical contribution of Ptpn11 mutations in the bone marrow microenvironment to leukaemogenesis and identifies CCL3 as a potential therapeutic target for controlling leukaemic progression in Noonan syndrome and for improving stem cell transplantation therapy in Noonan-syndrome-associated leukaemias. PMID: 27783593
    24. SHP2 is a potential positive regulator of eosinophil differentiation, and inhibition of SHP2 specifically in myeloid cells eventually alleviates eosinophilic airway inflammation. PMID: 27054330
    25. lack of Shp2 in orofacial cartilage led to severe defects of ciliogenesis. PMID: 25919282
    26. results collectively suggest that Shp2 is a critical signaling protein that is required to maintain Sertoli cell function and could serve as a novel target for male infertility therapies PMID: 26265072
    27. PTPN11 is a central node in intrinsic and acquired resistance to targeted cancer drugs. PMID: 26365186
    28. SHP2 activity is elevated in astrocytes isolated from glioblastoma multiforme (GBM)-prone H-Ras(12V) knock-in mice. PMID: 26617336
    29. find that Shp2 is distributed to the kinetochore, centrosome, spindle midzone, and midbody PMID: 26755576
    30. Mouse podocytes lacking Shp2 do not develop foot process spreading when subjected to podocyte injury in vivo using protamine sulfate or nephrotoxic serum. PMID: 26644409
    31. Leukemogenic Ptpn11 allele causes defective erythropoiesis in mice. PMID: 25289670
    32. SHP2 silencing in P815 mouse mastocytoma cell line harboring KITD814Y mutation results in impaired signaling to ERK, Btk, Lyn and STAT5 pathways, and reduced rates of cell growth and colony formation. PMID: 25026279
    33. Shp2 regulates IEC homeostasis through activation of Ras and thereby protects against the development of colitis. PMID: 24675817
    34. Loss of SHP-2 activity in CD4+ T cells promotes melanoma progression and metastasis. PMID: 24088816
    35. Overexpression of Shp2 increased the size of atrioventricular endocardial cushion by 80% at mid-gestation. PMID: 25359717
    36. Mutated Ptpn11 alters leukemic stem cell frequency and reduces the sensitivity of acute myeloid leukemia cells to Mcl1 inhibition. PMID: 25650089
    37. SHP2 activation offers the pulmonary endothelium protection against barrier permeability mediators downstream of the FAK signaling pathway. PMID: 25317600
    38. Loss of Shp2 is associated with osteopetrosis. PMID: 25593124
    39. Together these data reveal a mechanism by which SHP-2 mediates the activation of Syk in response to fungal infection. PMID: 25915733
    40. Shp2 contributes to regulation of Erk activation and synaptic plasticity in postmitotic forebrain neurons and thereby controls locomotor activity and memory formation. PMID: 25713104
    41. SHP2 mutation is associated with LEOPARD syndrome and confers leanness and protection from diet-induced obesity in a mouse model PMID: 25288766
    42. Shp2 associates with FRS2alpha and promotes the receptor activation and signal relay to several pathways. PMID: 24981838
    43. SHP2 activation is upregulated with trichomide A, a natural cyclodepsipeptide, which exerts immunosuppressive activity against activated T lymphocytes in contact dermatitis PMID: 24933319
    44. Shp2 signaling in POMC neurons contributes to the long-term blood pressure and antidiabetic actions of leptin and may play a modest role in normal regulation of body weight. PMID: 25339680
    45. Data suggest an important role of SHP2 in the molecular etiology of breast tumor growth. PMID: 24858400
    46. We found that compared to the normal colon tissues, SHP-2 significantly decreased in mice colon tumor tissues. PMID: 24439672
    47. we performed lineage tracing to show that exostoses and enchondromas in mice likely contain mixtures of wild-type and SHP2-deficient chondrocytes. PMID: 24875294
    48. these findings define a role for Shp2 in alveolar macrophages and reveal that Shp2 is required to inhibit the progression of M2-associated pulmonary fibrosis. PMID: 25127857
    49. Results reveal a critical role for SHP-2 in regulating experimental autoimmune encephalomyelitis pathogenesis. PMID: 24372081
    50. SHP2 is essential for spermatogonial stem cells to maintain fertility. PMID: 24123360

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class 2 subfamily
  • 组织特异性：
    Highly expressed in brain, heart and kidney.
  • 数据库链接：

    KEGG: mmu:19247

    STRING: 10090.ENSMUSP00000058757

    UniGene: Mm.8681